PMID- 36661785
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 2310-2861 (Electronic)
IS  - 2310-2861 (Linking)
VI  - 9
IP  - 1
DP  - 2022 Dec 26
TI  - Physicochemical and Fibril Formation Properties of Pufferfish (Takifugu obscurus) 
      Skin Collagen from Solvent Extraction in Different Conditions.
LID - 10.3390/gels9010017 [doi]
LID - 17
AB  - Acid-solubilized (ASC) and pepsin-solubilized collagen (PSC) extracted at 4  degrees C 
      (ASC-4 and PSC-4), 12  degrees C (ASC-12 and PSC-12), and 20  degrees C (ASC-20 and PSC-20) from 
      the skin of farmed pufferfish (Takifugu obscurus) was characterized by 
      SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier-transform infrared 
      spectroscopy (FTIR), and fibril-forming tests. The results indicate that 
      extraction at 12  degrees C can effectively improve the extraction efficiency of natural 
      collagen compared with extraction at 4  degrees C. However, extraction at 20  degrees C results 
      in a decrease in molecular integrity, thus, inducing the resultant collagen to 
      degrade or even lose fibril-forming ability. Transmission electron microscope 
      (TEM) images revealed that ASC-4, PSC-4, ASC-12, and PSC-12 can assemble into 
      fibrils with D-periodicities, and ASC-20 associated into molecular aggregates 
      alongside partial D-banded fibrils, while no well-defined fibrils were observed 
      in PSC-20. Scanning electron microscope (SEM) analysis confirmed the well-defined 
      fibril morphologies of ASC-4, PSC-4, ASC-12, and PSC-12 with imino acid contents 
      between 190.0 and 197.8 residues/1000 residues. The denaturation temperature of 
      ASC-4, PSC-4, ASC-12 and PSC-12 was 30.0, 27.6, 25.9 and 22.7  degrees C, respectively. 
      This study indicates that ASC and PSC extracted at 4  degrees C and 12  degrees C could be 
      alternatives to terrestrial collagens for industrial applications.
FAU - Wang, Shanshan
AU  - Wang S
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Zhou, Deqing
AU  - Zhou D
AUID- ORCID: 0000-0002-9035-5440
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
AD  - Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine 
      Science and Technology (Qingdao), Qingdao 266237, China.
FAU - Liu, Nan
AU  - Liu N
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
AD  - Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine 
      Science and Technology (Qingdao), Qingdao 266237, China.
FAU - Sun, Yong
AU  - Sun Y
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Sun, Guohui
AU  - Sun G
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
LA  - eng
GR  - 2019ZDCX011/Science and Technology Program of Yantai City/
PT  - Journal Article
DEP - 20221226
PL  - Switzerland
TA  - Gels
JT  - Gels (Basel, Switzerland)
JID - 101696925
PMC - PMC9857395
OTO - NOTNLM
OT  - acid-solubilized collagen
OT  - fibril formation
OT  - microstructural characteristic
OT  - pepsin-solubilized collagen
OT  - pufferfish skin
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/21 06:00
MHDA- 2023/01/21 06:01
PMCR- 2022/12/26
CRDT- 2023/01/20 09:43
PHST- 2022/11/28 00:00 [received]
PHST- 2022/12/19 00:00 [revised]
PHST- 2022/12/22 00:00 [accepted]
PHST- 2023/01/20 09:43 [entrez]
PHST- 2023/01/21 06:00 [pubmed]
PHST- 2023/01/21 06:01 [medline]
PHST- 2022/12/26 00:00 [pmc-release]
AID - gels9010017 [pii]
AID - gels-09-00017 [pii]
AID - 10.3390/gels9010017 [doi]
PST - epublish
SO  - Gels. 2022 Dec 26;9(1):17. doi: 10.3390/gels9010017.