PMID- 36662209
OWN - NLM
STAT- MEDLINE
DCOM- 20230131
LR  - 20240109
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 21
IP  - 1
DP  - 2022 Dec 31
TI  - FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis 
      integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory 
      Potency In Vitro and In Vivo.
LID - 10.3390/md21010036 [doi]
LID - 36
AB  - The aim of this study was to evaluate the antiangiogenic and immunomodulatory 
      potential of sulfated polysaccharides from the marine algae Macrocystis 
      integrifolia characterized by FTIR. The cytotoxicity of sulfated polysaccharides 
      was evaluated using the 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium 
      bromide (MTT) assay. Antiangiogenic activity was evaluated using the chicken 
      chorioallantoic membrane (CAM) assay. Immunomodulatory activity was determined on 
      macrophage functionality and allergic response. The results showed that sulfated 
      polysaccharides significantly decreased angiogenesis in chicken chorioallantoic 
      membranes (p < 0.05). Likewise, they inhibited in vivo chemotaxis and in vitro 
      phagocytosis, the transcription process of genes that code the enzymes 
      cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and nitric oxide synthase-2 
      (NOS-2) and the nuclear factor kappa-light chain enhancer of activated B cells 
      (NF-kappaB), showing immunomodulatory properties on the allergic response, as well as 
      an in vivo inhibitory effect in the ovalbumin-induced inflammatory allergy model 
      (OVA) and inhibited lymphocyte proliferation specific to the OVA antigen in 
      immunized mice. Finally, these compounds inhibited the histamine-induced skin 
      reaction in rats, the production of immunoglobulin E (IgE) in mice, and the 
      passive response to skin anaphylaxis in rats. Therefore, the results of this 
      research showed the potential of these compounds to be a promising source for the 
      development of antiangiogenic and immunomodulatory drugs.
FAU - Soto-Vasquez, Marilu Roxana
AU  - Soto-Vasquez MR
AUID- ORCID: 0000-0002-1178-4678
AD  - Facultad de Farmacia y Bioquimica, Universidad Nacional de Trujillo, Av. Juan 
      Pablo II, Trujillo 13011, Peru.
FAU - Alvarado-Garcia, Paul Alan Arkin
AU  - Alvarado-Garcia PAA
AUID- ORCID: 0000-0003-1641-207X
AD  - Escuela de Medicina, Universidad Cesar Vallejo, Av. Larco s/n, Trujillo 13011, 
      Peru.
FAU - Youssef, Fadia S
AU  - Youssef FS
AUID- ORCID: 0000-0002-5871-2639
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Abbasia, 
      Cairo 11566, Egypt.
FAU - Ashour, Mohamed L
AU  - Ashour ML
AUID- ORCID: 0000-0002-9270-6267
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Abbasia, 
      Cairo 11566, Egypt.
AD  - Pharmacy Program, Department of Pharmaceutical Sciences, Batterjee Medical 
      College, Jeddah 21442, Saudi Arabia.
FAU - Bogari, Hanin A
AU  - Bogari HA
AUID- ORCID: 0000-0001-5974-6235
AD  - Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, 
      Jeddah 21589, Saudi Arabia.
FAU - Elhady, Sameh S
AU  - Elhady SS
AUID- ORCID: 0000-0003-3799-0581
AD  - Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, 
      Jeddah 21589, Saudi Arabia.
LA  - eng
GR  - Institutional Fund Projects under grant no. (IFPIP:444-249-1443)/This research 
      work was funded by Institutional Fund Projects under grant no. 
      (IFPIP:444-249-1443). The authors gratefully acknowledge technical and financial 
      support provided by the Ministry of Education and King Abdulaziz University, DSR, 
      Jeddah, Saudi A/
PT  - Journal Article
DEP - 20221231
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (NF-kappa B)
RN  - 0 (Polysaccharides)
RN  - 0 (Sulfates)
RN  - 0 (Angiogenesis Inhibitors)
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Rats
MH  - *Macrocystis
MH  - NF-kappa B
MH  - Polysaccharides/pharmacology
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Sulfates
MH  - Angiogenesis Inhibitors/pharmacology
MH  - Immunologic Factors/pharmacology
PMC - PMC9863126
OTO - NOTNLM
OT  - Macrocystis integrifolia
OT  - algae
OT  - anti-allergic
OT  - antiangiogenic
OT  - drug discovery
OT  - polysaccharides
OT  - public health
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/21 06:00
MHDA- 2023/01/25 06:00
PMCR- 2022/12/31
CRDT- 2023/01/20 11:03
PHST- 2022/11/18 00:00 [received]
PHST- 2022/12/27 00:00 [revised]
PHST- 2022/12/29 00:00 [accepted]
PHST- 2023/01/20 11:03 [entrez]
PHST- 2023/01/21 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/12/31 00:00 [pmc-release]
AID - md21010036 [pii]
AID - marinedrugs-21-00036 [pii]
AID - 10.3390/md21010036 [doi]
PST - epublish
SO  - Mar Drugs. 2022 Dec 31;21(1):36. doi: 10.3390/md21010036.