PMID- 36669744
OWN - NLM
STAT- MEDLINE
DCOM- 20230224
LR  - 20230320
IS  - 1569-1802 (Electronic)
IS  - 0945-053X (Linking)
VI  - 116
DP  - 2023 Feb
TI  - Infiltrating CD8+ T cells and M2 macrophages are retained in tumor matrix tracks 
      enriched in low tension fibronectin fibers.
PG  - 1-27
LID - S0945-053X(23)00002-1 [pii]
LID - 10.1016/j.matbio.2023.01.002 [doi]
AB  - Tracks rich in matrix and cells, as described in several cancer types, have 
      immunosuppressive functions and separate tumor nests and stroma, yet their origin 
      is unknown. Immunostainings of cryosections from mouse breast tumors show that 
      these tracks are bordered by an endothelial-like basement membrane, filled with 
      fibers of collagen adjacent to tenascin-C (TNC) and low-tension fibronectin (Fn) 
      fibers. While present in early-stage tumors and maturing with time, tracks still 
      form under TNC KO conditions, however, host (not tumor cell)-derived TNC is 
      important for track maturation. Tumor infiltrating leukocytes (mostly M2 
      macrophages and CD8+ T cells) are retained in tracks of early-stage tumors. 
      Following track maturation, retained tumor infiltrating leukocyte (TIL) numbers 
      get reduced and more CD8+ TIL enter the tumor nests in the absence of TNC. As 
      these tracks are enriched with platelets and fibrinogen and have a demarcating 
      endothelial-like basement membrane often adjacent to endothelial cells, this 
      suggests a role of blood vessels in the formation of these tracks. The Fn fiber 
      tension probe FnBPA5 colocalizes with TNC and immune cells in the tracks and 
      shows decreased binding in tracks lacking TNC. Consequently, FnBPA5 can serve as 
      probe for tumor matrix tracks that have immune suppressive properties.
CI  - Copyright (c) 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Fonta, Charlotte M
AU  - Fonta CM
AD  - Laboratory of Applied Mechanobiology, Institute of Translational Medicine, 
      Department of Health Sciences and Technology, ETH Zurich, Vladimir Prelog Weg, 
      Zurich CH-8093, Switzerland.
FAU - Loustau, Thomas
AU  - Loustau T
AD  - The Tumor Microenvironment Laboratory, INSERM U1109, Hopital Civil, Institut d' 
      Hematologie et d'Immunologie, 1 Place de l'Hopital, Strasbourg 67091, France; 
      Universite Strasbourg, Strasbourg 67000, France; Federation de Medecine 
      Translationnelle de Strasbourg (FMTS), Strasbourg 67000, France.
FAU - Li, Chengbei
AU  - Li C
AD  - The Tumor Microenvironment Laboratory, INSERM U1109, Hopital Civil, Institut d' 
      Hematologie et d'Immunologie, 1 Place de l'Hopital, Strasbourg 67091, France; 
      Universite Strasbourg, Strasbourg 67000, France; Federation de Medecine 
      Translationnelle de Strasbourg (FMTS), Strasbourg 67000, France.
FAU - Poilil Surendran, Suchithra
AU  - Poilil Surendran S
AD  - The Tumor Microenvironment Laboratory, INSERM U1109, Hopital Civil, Institut d' 
      Hematologie et d'Immunologie, 1 Place de l'Hopital, Strasbourg 67091, France; 
      Universite Strasbourg, Strasbourg 67000, France; Federation de Medecine 
      Translationnelle de Strasbourg (FMTS), Strasbourg 67000, France.
FAU - Hansen, Uwe
AU  - Hansen U
AD  - Institute for Musculoskeletal Medicine (IMM), University Hospital Muenster, 
      Muenster, Federal Republic of Germany.
FAU - Murdamoothoo, Devadarssen
AU  - Murdamoothoo D
AD  - The Tumor Microenvironment Laboratory, INSERM U1109, Hopital Civil, Institut d' 
      Hematologie et d'Immunologie, 1 Place de l'Hopital, Strasbourg 67091, France; 
      MN3T (The Microenvironmental Niche in Tumorigenesis and Targeted Therapy), INSERM 
      U1109, 3 avenue Moliere, Strasbourg, Hautepierre, France; Universite Strasbourg, 
      Strasbourg 67000, France; Federation de Medecine Translationnelle de Strasbourg 
      (FMTS), Strasbourg 67000, France.
FAU - Benn, Mario C
AU  - Benn MC
AD  - Laboratory of Applied Mechanobiology, Institute of Translational Medicine, 
      Department of Health Sciences and Technology, ETH Zurich, Vladimir Prelog Weg, 
      Zurich CH-8093, Switzerland.
FAU - Velazquez-Quesada, Ines
AU  - Velazquez-Quesada I
AD  - The Tumor Microenvironment Laboratory, INSERM U1109, Hopital Civil, Institut d' 
      Hematologie et d'Immunologie, 1 Place de l'Hopital, Strasbourg 67091, France; 
      MN3T (The Microenvironmental Niche in Tumorigenesis and Targeted Therapy), INSERM 
      U1109, 3 avenue Moliere, Strasbourg, Hautepierre, France; Universite Strasbourg, 
      Strasbourg 67000, France; Federation de Medecine Translationnelle de Strasbourg 
      (FMTS), Strasbourg 67000, France.
FAU - Carapito, Raphael
AU  - Carapito R
AD  - Universite Strasbourg, Strasbourg 67000, France; Federation de Medecine 
      Translationnelle de Strasbourg (FMTS), Strasbourg 67000, France; Platform 
      GENOMAX, INSERM UMR_S 1109, Institut thematique interdisciplinaire (ITI) de 
      Medecine de Precision de Strasbourg, Transplantex NG, Faculte de Medecine, 
      Federation Hospitalo-Universitaire OMICARE, LabEx TRANSPLANTEX, Strasbourg 67091, 
      France.
FAU - Orend, Gertraud
AU  - Orend G
AD  - The Tumor Microenvironment Laboratory, INSERM U1109, Hopital Civil, Institut d' 
      Hematologie et d'Immunologie, 1 Place de l'Hopital, Strasbourg 67091, France; 
      MN3T (The Microenvironmental Niche in Tumorigenesis and Targeted Therapy), INSERM 
      U1109, 3 avenue Moliere, Strasbourg, Hautepierre, France; Universite Strasbourg, 
      Strasbourg 67000, France; Federation de Medecine Translationnelle de Strasbourg 
      (FMTS), Strasbourg 67000, France. Electronic address: gertraud.orend@inserm.fr.
FAU - Vogel, Viola
AU  - Vogel V
AD  - Laboratory of Applied Mechanobiology, Institute of Translational Medicine, 
      Department of Health Sciences and Technology, ETH Zurich, Vladimir Prelog Weg, 
      Zurich CH-8093, Switzerland. Electronic address: viola.vogel@hest.ethz.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230118
PL  - Netherlands
TA  - Matrix Biol
JT  - Matrix biology : journal of the International Society for Matrix Biology
JID - 9432592
RN  - 0 (Fibronectins)
RN  - 0 (Tenascin)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Fibronectins/metabolism
MH  - Endothelial Cells/metabolism
MH  - *Neoplasms/pathology
MH  - Macrophages/metabolism
MH  - Tenascin/metabolism
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - T-Lymphocytes/metabolism
OTO - NOTNLM
OT  - CD8+ T cells
OT  - Cancer
OT  - Endothelial-mesenchymal transition (EndoMT)
OT  - Fibronectin binding peptide FnBPA5
OT  - M2 macrophages
OT  - Tenascin-C
OT  - Tumor matrix tracks
EDAT- 2023/01/21 06:00
MHDA- 2023/02/25 06:00
CRDT- 2023/01/20 19:26
PHST- 2022/05/11 00:00 [received]
PHST- 2022/12/31 00:00 [revised]
PHST- 2023/01/12 00:00 [accepted]
PHST- 2023/01/21 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2023/01/20 19:26 [entrez]
AID - S0945-053X(23)00002-1 [pii]
AID - 10.1016/j.matbio.2023.01.002 [doi]
PST - ppublish
SO  - Matrix Biol. 2023 Feb;116:1-27. doi: 10.1016/j.matbio.2023.01.002. Epub 2023 Jan 
      18.