PMID- 36670940
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Dec 29
TI  - Ishige okamurae Attenuates Neuroinflammation and Cognitive Deficits in Mice 
      Intracerebroventricularly Injected with LPS via Regulating TLR-4/MyD88-Dependent 
      Pathways.
LID - 10.3390/antiox12010078 [doi]
LID - 78
AB  - Neuroinflammation is one of the critical causes of neuronal loss and cognitive 
      impairment. We aimed to evaluate the anti-neuroinflammatory properties of Ishige 
      okamuae using mice intracerebroventricularly injected with lipopolysaccharides 
      (LPS) and LPS-treated C6 glioma cells. We found that the short- and long-term 
      memory deficits of LPS-injected mice were improved by oral administration of 
      Ishige okamurae extracts (IOE). LPS-induced neuronal loss, increase in amyloid-beta 
      plaque, and expression of COX-2 and iNOS were restored by IOE. In addition, 
      LPS-induced activation of Toll-like receptor-4 (TLR-4) and its downstream 
      molecules, such as MyD88, NFkappaB, and mitogen-activated protein kinases (MAPKs), 
      were significantly attenuated in the brains of mice fed with IOE. We found that 
      pretreatment of IOE to C6 glioma cells ameliorated LPS-induced expression of 
      TLR-4 and its inflammatory cascades, such as MyD88 expression, reactive oxygen 
      species production, MAPKs phosphorylation, and NFkappaB phosphorylation with 
      consequent downregulation of COX-2, iNOS, proinflammatory cytokines, and nitric 
      oxide expression. Furthermore, IOE (0.2 microg/mL) was found to have equivalent 
      efficacy with 10 muM of MyD88 inhibitor in preventing LPS-induced inflammatory 
      responses in C6 glioma cells. Taken together, these results strongly suggest that 
      IOE could be developed as a promising anti-neuroinflammatory agent which is able 
      to control the TLR-4/MyD88-dependent signaling pathways.
FAU - Kwon, Oh-Yun
AU  - Kwon OY
AD  - Department of Nano-Bioengineering, Incheon National University, 119 Academy-ro, 
      Incheon 22012, Republic of Korea.
FAU - Lee, Seung-Ho
AU  - Lee SH
AUID- ORCID: 0000-0002-9941-4195
AD  - Department of Nano-Bioengineering, Incheon National University, 119 Academy-ro, 
      Incheon 22012, Republic of Korea.
LA  - eng
GR  - Incheon Nation University research grant in 2021/Incheon National University/
PT  - Journal Article
DEP - 20221229
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC9854571
OTO - NOTNLM
OT  - Ishige okamurae
OT  - MyD88
OT  - TLR-4
OT  - cognitive deficits
OT  - lipopolysaccharide
OT  - neuroinflammation
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/22 06:01
PMCR- 2022/12/29
CRDT- 2023/01/21 01:04
PHST- 2022/12/07 00:00 [received]
PHST- 2022/12/22 00:00 [revised]
PHST- 2022/12/26 00:00 [accepted]
PHST- 2023/01/21 01:04 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/22 06:01 [medline]
PHST- 2022/12/29 00:00 [pmc-release]
AID - antiox12010078 [pii]
AID - antioxidants-12-00078 [pii]
AID - 10.3390/antiox12010078 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Dec 29;12(1):78. doi: 10.3390/antiox12010078.