PMID- 36672647
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230123
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 11
IP  - 1
DP  - 2023 Jan 5
TI  - Fetal Growth Restriction Impairs Lung Function and Neurodevelopment in an Early 
      Preterm Rabbit Model.
LID - 10.3390/biomedicines11010139 [doi]
LID - 139
AB  - We previously reported the multi-system sequelae of fetal growth restriction, 
      induced by placental underperfusion, in near-term born rabbits, in the immediate 
      neonatal period and up to pre-adolescence. Herein, we describe the pulmonary and 
      neurodevelopmental consequences of FGR in rabbits born preterm. We hypothesize 
      that FGR has an additional detrimental effect on prematurity in both pulmonary 
      function and neurodevelopment. FGR was induced at gestational day (GD) 25 by 
      placental underperfusion, accomplished by partial uteroplacental vessel ligation 
      in one uterine horn. Rabbits were delivered by cesarean section at GD 29, and 
      placentas were harvested for histology. Neonates underwent neurobehavioral or 
      pulmonary functional assessment at postnatal day 1, followed by brain or lung 
      harvesting, respectively. The neurodevelopmental assessment included 
      neurobehavioral testing and multiregional quantification of cell density and 
      apoptosis in the brain. Lung assessment included functional testing, alveolar 
      morphometry, and airway histology. FGR was associated with higher perinatal 
      mortality, lower birth and placental weight, and a similar brain-to-body weight 
      ratio compared to controls. Placental underperfusion decreased labyrinth and 
      junction zone volumes in FGR placentas. FGR impaired pulmonary function, depicted 
      by higher parenchymal resistance, damping, and elastance. Alveolar morphometry 
      and airway smooth muscle content were comparable between groups. Neurobehavioral 
      tests showed motoric and sensorial impairment in FGR rabbits. In FGR brains, cell 
      density was globally reduced, with higher apoptosis in selected areas. In 
      conclusion, in preterm-born rabbits, placental underperfusion leads to higher 
      mortality, FGR, and impaired lung and brain development in early assessment. This 
      study complements previous findings of placental, pulmonary, and 
      neurodevelopmental impairment in near-term born rabbits in this model.
FAU - Valenzuela, Ignacio
AU  - Valenzuela I
AUID- ORCID: 0000-0002-7277-2152
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
FAU - Zapletalova, Katerina
AU  - Zapletalova K
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
AD  - Third Faculty of Medicine, Institute for the Care of Mother and Child, Charles 
      University, 147 10 Prague, Czech Republic.
FAU - Greyling, Marnel
AU  - Greyling M
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
FAU - Regin, Yannick
AU  - Regin Y
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
FAU - Gie, Andre
AU  - Gie A
AD  - Department of Paediatrics and Child Health, Faculty of Medicine and Health 
      Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town 7505, South 
      Africa.
FAU - Basurto, David
AU  - Basurto D
AUID- ORCID: 0000-0002-2022-9027
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
FAU - Deprest, Jan
AU  - Deprest J
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
AD  - Department of Obstetrics and Gynecology, Division Woman and Child, University 
      Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium.
FAU - van der Merwe, Johannes
AU  - van der Merwe J
AUID- ORCID: 0000-0002-1381-4033
AD  - Cluster Woman and Child, Department of Development and Regeneration, Group 
      Biomedical Sciences, KU Leuven Herestraat 49, 3000 Leuven, Belgium.
AD  - Department of Obstetrics and Gynecology, Division Woman and Child, University 
      Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium.
LA  - eng
GR  - 765274/European Union's Horizon 2020 research and innovation programme/
PT  - Journal Article
DEP - 20230105
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC9855731
OTO - NOTNLM
OT  - animal model
OT  - fetal growth restriction
OT  - lung development
OT  - neurodevelopment
OT  - placental insufficiency
OT  - preclinical
OT  - prematurity
OT  - rabbit
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/22 06:01
PMCR- 2023/01/05
CRDT- 2023/01/21 01:13
PHST- 2022/12/06 00:00 [received]
PHST- 2022/12/26 00:00 [revised]
PHST- 2022/12/29 00:00 [accepted]
PHST- 2023/01/21 01:13 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/22 06:01 [medline]
PHST- 2023/01/05 00:00 [pmc-release]
AID - biomedicines11010139 [pii]
AID - biomedicines-11-00139 [pii]
AID - 10.3390/biomedicines11010139 [doi]
PST - epublish
SO  - Biomedicines. 2023 Jan 5;11(1):139. doi: 10.3390/biomedicines11010139.