PMID- 36672747
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230201
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 14
IP  - 1
DP  - 2022 Dec 20
TI  - The Role of Pancreatic Fatty Acid Synthesis in Islet Morphology and Function 
      after Caloric Restriction or Roux-En-Y Gastric Bypass Surgery in Mice.
LID - 10.3390/genes14010005 [doi]
LID - 5
AB  - BACKGROUND: Both caloric restriction (CR) and Roux-en-Y gastric bypass (RYGB) are 
      practical interventions for type 2 diabetes mellitus (T2DM), while the molecular 
      mechanisms of CR and RYGB regarding glycemic control are still poorly understood. 
      Here, we explore the effects and underlying mechanisms of CR and RYGB on beta-cell 
      area and function. METHODS: Average islet size was measured by histological 
      analysis. The pancreatic lipid content was detected by using a commercial lipid 
      assay kit. The expression levels of lipogenic transcription factors and enzymes 
      in mouse pancreas were determined by quantitative PCR, Western blot, and 
      immunofluorescence. RESULTS: CR decreased the mean size of islets and pancreatic 
      insulin production in both regular diet-fed and high-fat diet-fed mice. Increased 
      beta-cell apoptosis was detected in the calorie-restricted mice. Interestingly, the 
      lipogenic transcription factors and enzymes such as SREBP1c, PPARgamma, FASN and ACC 
      were upregulated in the pancreas after CR. In contrast to CR, RYGB decreased the 
      apoptosis of beta-cells and the expression of fatty acid synthase. CONCLUSIONS: 
      Pancreatic fatty acid synthesis is critical to the beta-cell function after CR and 
      RYGB.
FAU - Mo, Haocong
AU  - Mo H
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Zhang, Mengyuan
AU  - Zhang M
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Qiu, Zirui
AU  - Qiu Z
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Li, Yilin
AU  - Li Y
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Zhang, Zhejiao
AU  - Zhang Z
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Li, Yanting
AU  - Li Y
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
FAU - Xu, Geyang
AU  - Xu G
AUID- ORCID: 0000-0002-5542-1350
AD  - Department of Physiology, School of Medicine, Jinan University, 601 Huangpu 
      Avenue West, Guangzhou 510632, China.
AD  - Center for Clinical Epidemiology and Methodology (CCEM), Guangdong Second 
      Provincial General Hospital, Guangzhou 510317, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221220
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (Transcription Factors)
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Gastric Bypass
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Caloric Restriction
MH  - Pancreas/metabolism
MH  - Transcription Factors
MH  - Fatty Acids
MH  - Lipids
PMC - PMC9858859
OTO - NOTNLM
OT  - Roux-en-Y gastric bypass
OT  - apoptosis
OT  - caloric restriction
OT  - diabetes
OT  - fatty acid synthesis
OT  - beta-cell
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/25 06:00
PMCR- 2022/12/20
CRDT- 2023/01/21 01:14
PHST- 2022/10/03 00:00 [received]
PHST- 2022/12/11 00:00 [revised]
PHST- 2022/12/19 00:00 [accepted]
PHST- 2023/01/21 01:14 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/12/20 00:00 [pmc-release]
AID - genes14010005 [pii]
AID - genes-14-00005 [pii]
AID - 10.3390/genes14010005 [doi]
PST - epublish
SO  - Genes (Basel). 2022 Dec 20;14(1):5. doi: 10.3390/genes14010005.