PMID- 36673343
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 12
IP  - 2
DP  - 2023 Jan 5
TI  - Preparation Process Optimization of Peptides from Agaricus blazei Murrill, and 
      Comparison of Their Antioxidant and Immune-Enhancing Activities Separated by 
      Ultrafiltration Membrane Technology.
LID - 10.3390/foods12020251 [doi]
LID - 251
AB  - Agaricus blazei murrill (ABM), a large fungus, is reported to have extensive 
      biological activities but the antioxidant and immune-regulatory capacities have 
      been less studied and the components responsible for the functions are unclear. 
      This study prepared ABM peptides (ABMP) using ultrasound-assisted enzymatic 
      extraction (UAEE) strategy and cascade ultrafiltration (UF) membrane technology. 
      The UAEE extraction conditions were optimized using response surface methodology 
      (RSM) with four factors and three levels to achieve the maximum ABMP yield 
      (34.03%); the optimal conditions were an enzyme amount of 4%, ratio of ABM to 
      water of 1:30, ultrasonic power of 360 W, and ultrasonic time of 30 min. Four 
      ABMP fractions were obtained after UF with different pore size and their 
      antioxidant and immune-regulatory abilities were evaluated and compared. The 
      results showed that they could effectively scavenge DPPH, hydroxyl, and ABTS 
      radicals, especially for ABMP-2; the scavenging rate of the above radicals were 
      79.31%, 63.60%, and 96.08%, respectively. In addition, four ABMP fractions also 
      activated macrophage activity through strengthening phagocytosis and the 
      production of NO, IL-6, IL-1beta, and TNF-alpha in a dose-dependent manner. Notably, the 
      ABMP-2 fraction with a MW of 3-5 kDa and peptide purity of 82.88% was found to 
      have the best effect, showing the maximum phagocytosis (189.37%) as well as NO 
      (7.98 muM), IL-6 (195.05 pg/mL), IL-1beta (876.15 pg/mL), and TNF-alpha (1620 pg/mL) 
      secretion at a treatment concentration of 150 mug/mL. The findings indicated that 
      the ABMP, especially for the separate ABMP-2, could be used as dietary 
      supplements and have the potential to be exploited as immune-enhancing agents.
FAU - Zou, Xian-Guo
AU  - Zou XG
AUID- ORCID: 0000-0002-1760-5618
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
AD  - Key Laboratory of Food Macromolecular Resources Processing Technology Research, 
      Zhejiang University of Technology, China National Light Industry, Hangzhou 
      310014, China.
FAU - Chi, Yun
AU  - Chi Y
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
FAU - Cao, Yu-Qin
AU  - Cao YQ
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
FAU - Zheng, Miao
AU  - Zheng M
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
FAU - Deng, Ze-Yuan
AU  - Deng ZY
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang 330047, China.
FAU - Cai, Ming
AU  - Cai M
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
AD  - Key Laboratory of Food Macromolecular Resources Processing Technology Research, 
      Zhejiang University of Technology, China National Light Industry, Hangzhou 
      310014, China.
FAU - Yang, Kai
AU  - Yang K
AUID- ORCID: 0000-0001-5571-6645
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
AD  - Key Laboratory of Food Macromolecular Resources Processing Technology Research, 
      Zhejiang University of Technology, China National Light Industry, Hangzhou 
      310014, China.
FAU - Sun, Pei-Long
AU  - Sun PL
AUID- ORCID: 0000-0003-1963-1120
AD  - College of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou 310014, China.
AD  - Key Laboratory of Food Macromolecular Resources Processing Technology Research, 
      Zhejiang University of Technology, China National Light Industry, Hangzhou 
      310014, China.
LA  - eng
GR  - 2022C04021/"Pioneer" and "Leading Goose" R&D Program of Zhejiang/
PT  - Journal Article
DEP - 20230105
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC9858576
OTO - NOTNLM
OT  - Agaricus blazei
OT  - antioxidant activity
OT  - immune-enhancing activity
OT  - peptide
OT  - ultrafiltration technology
OT  - ultrasound-assisted enzymatic extraction
COIS- All the authors declare no conflict of interests.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/22 06:01
PMCR- 2023/01/05
CRDT- 2023/01/21 01:17
PHST- 2022/12/02 00:00 [received]
PHST- 2022/12/24 00:00 [revised]
PHST- 2022/12/25 00:00 [accepted]
PHST- 2023/01/21 01:17 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/22 06:01 [medline]
PHST- 2023/01/05 00:00 [pmc-release]
AID - foods12020251 [pii]
AID - foods-12-00251 [pii]
AID - 10.3390/foods12020251 [doi]
PST - epublish
SO  - Foods. 2023 Jan 5;12(2):251. doi: 10.3390/foods12020251.