PMID- 36674450
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230201
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 2
DP  - 2023 Jan 4
TI  - F(4)-Neuroprostane Effects on Human Sperm.
LID - 10.3390/ijms24020935 [doi]
LID - 935
AB  - Swim-up selected human sperm were incubated with 7 ng F(4)-neuroprostanes 
      (F(4)-NeuroPs) for 2 and 4 h. Sperm motility and membrane mitochondrial potential 
      (MMP) were evaluated. The percentage of reacted acrosome was assessed by pisum 
      sativum agglutinin (PSA). Chromatin integrity was detected using the acridine 
      orange (AO) assay and localization of the ryanodine receptor was performed by 
      immunofluorescence analysis. Sperm progressive motility (p = 0.02) and the 
      percentage of sperm showing a strong MMP signal (p = 0.012) significantly 
      increased after 2 h F(4)-NeuroP incubation compared to control samples. The AO 
      assay did not show differences in the percentage of sperm with dsDNA between 
      treated or control samples. Meanwhile, a significantly higher number of sperm 
      with reacted acrosomes was highlighted by PSA localization after 4 h F(4)-NeuroP 
      incubation. Finally, using an anti-ryanodine antibody, the immunofluorescence 
      signal was differentially distributed at 2 and 4 h: a strong signal was evident 
      in the midpiece and postacrosomal sheath (70% of sperm) at 2 h, whereas a dotted 
      one appeared at 4 h (53% of sperm). A defined concentration of F(4)-NeuroPs in 
      seminal fluid may induce sperm capacitation via channel ions present in sperm 
      cells, representing an aid during in vitro sperm preparation that may increase 
      the positive outcome of assisted fertilization.
FAU - Moretti, Elena
AU  - Moretti E
AUID- ORCID: 0000-0002-9467-0501
AD  - Department of Molecular and Developmental Medicine, Policlinico Le Scotte, 
      University of Siena, 53100 Siena, Italy.
FAU - Signorini, Cinzia
AU  - Signorini C
AUID- ORCID: 0000-0003-0006-9414
AD  - Department of Molecular and Developmental Medicine, Policlinico Le Scotte, 
      University of Siena, 53100 Siena, Italy.
FAU - Noto, Daria
AU  - Noto D
AD  - Department of Molecular and Developmental Medicine, Policlinico Le Scotte, 
      University of Siena, 53100 Siena, Italy.
FAU - Corsaro, Roberta
AU  - Corsaro R
AD  - Department of Molecular and Developmental Medicine, Policlinico Le Scotte, 
      University of Siena, 53100 Siena, Italy.
FAU - Micheli, Lucia
AU  - Micheli L
AUID- ORCID: 0000-0002-0306-9308
AD  - Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 
      Siena, Italy.
FAU - Durand, Thierry
AU  - Durand T
AD  - Institut des Biomolecules Max Mousseron (IBMM), Pole Chimie Balard Recherche, UMR 
      5247, CNRS, Universite de Montpellier, ENSCM, 34090 Montpellier, France.
FAU - Oger, Camille
AU  - Oger C
AUID- ORCID: 0000-0002-5177-5792
AD  - Institut des Biomolecules Max Mousseron (IBMM), Pole Chimie Balard Recherche, UMR 
      5247, CNRS, Universite de Montpellier, ENSCM, 34090 Montpellier, France.
FAU - Galano, Jean Marie
AU  - Galano JM
AD  - Institut des Biomolecules Max Mousseron (IBMM), Pole Chimie Balard Recherche, UMR 
      5247, CNRS, Universite de Montpellier, ENSCM, 34090 Montpellier, France.
FAU - Collodel, Giulia
AU  - Collodel G
AUID- ORCID: 0000-0003-1587-0159
AD  - Department of Molecular and Developmental Medicine, Policlinico Le Scotte, 
      University of Siena, 53100 Siena, Italy.
LA  - eng
PT  - Journal Article
DEP - 20230104
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Neuroprostanes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Humans
MH  - Male
MH  - *Neuroprostanes
MH  - Sperm Motility
MH  - Seeds
MH  - Spermatozoa
MH  - Acrosome
MH  - Acridine Orange
PMC - PMC9861396
OTO - NOTNLM
OT  - F4-neuroprostanes
OT  - acrosome reaction
OT  - capacitation
OT  - human sperm
OT  - in vitro study
OT  - ryanodine receptor
OT  - sperm motility
COIS- All authors declare that they do not have any conflict of interest that could 
      inappropriately influence this manuscript.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/25 06:00
PMCR- 2023/01/04
CRDT- 2023/01/21 01:24
PHST- 2022/11/05 00:00 [received]
PHST- 2022/12/20 00:00 [revised]
PHST- 2022/12/20 00:00 [accepted]
PHST- 2023/01/21 01:24 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2023/01/04 00:00 [pmc-release]
AID - ijms24020935 [pii]
AID - ijms-24-00935 [pii]
AID - 10.3390/ijms24020935 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jan 4;24(2):935. doi: 10.3390/ijms24020935.