PMID- 36674644
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230201
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 2
DP  - 2023 Jan 6
TI  - Preparation and In Vitro Characterization of Magnetic CS/PVA/HA/pSPIONs Scaffolds 
      for Magnetic Hyperthermia and Bone Regeneration.
LID - 10.3390/ijms24021128 [doi]
LID - 1128
AB  - Conventional bone cancer treatment often results in unwanted side effects, 
      critical-sized bone defects, and inefficient cancer-cell targeting. Therefore, 
      new approaches are necessary to better address bone cancer treatment and 
      patient's recovery. One solution may reside in the combination of bone 
      regeneration scaffolds with magnetic hyperthermia. By incorporating pristine 
      superparamagnetic iron oxide nanoparticles (pSPIONs) into additively manufactured 
      scaffolds we created magnetic structures for magnetic hyperthermia and bone 
      regeneration. For this, hydroxyapatite (HA) particles were integrated in a 
      polymeric matrix composed of chitosan (CS) and poly (vinyl alcohol) (PVA). Once 
      optimized, pSPIONs were added to the CS/PVA/HA paste at three different 
      concentrations (1.92, 3.77, and 5.54 wt.%), and subsequently additively 
      manufactured to form a scaffold. Results indicate that scaffolds containing 3.77 
      and 5.54 wt.% of pSPIONs, attained temperature increases of 6.6 and 7.5  degrees C in 
      magnetic hyperthermia testing, respectively. In vitro studies using human 
      osteosarcoma Saos-2 cells indicated that pSPIONs incorporation significantly 
      stimulated cell adhesion, proliferation and alkaline phosphatase (ALP) expression 
      when compared to CS/PVA/HA scaffolds. Thus, these results support that 
      CS/PVA/HA/pSPIONs scaffolds with pSPIONs concentrations above or equal to 3.77 
      wt.% have the potential to be used for magnetic hyperthermia and bone 
      regeneration.
FAU - Tavares, Francisco J T M
AU  - Tavares FJTM
AUID- ORCID: 0000-0002-5248-3097
AD  - i3N/CENIMAT, Department of Materials Science, NOVA School of Science and 
      Technology (FCT NOVA), Campus de Caparica, 2829-516 Caparica, Portugal.
FAU - Soares, Paula I P
AU  - Soares PIP
AUID- ORCID: 0000-0002-4975-7480
AD  - i3N/CENIMAT, Department of Materials Science, NOVA School of Science and 
      Technology (FCT NOVA), Campus de Caparica, 2829-516 Caparica, Portugal.
FAU - Silva, Jorge Carvalho
AU  - Silva JC
AUID- ORCID: 0000-0001-9959-4272
AD  - i3N/CENIMAT, Department of Physics, NOVA School of Science and Technology, Campus 
      de Caparica, 2829-516 Caparica, Portugal.
FAU - Borges, Joao Paulo
AU  - Borges JP
AUID- ORCID: 0000-0002-3996-6545
AD  - i3N/CENIMAT, Department of Materials Science, NOVA School of Science and 
      Technology (FCT NOVA), Campus de Caparica, 2829-516 Caparica, Portugal.
LA  - eng
GR  - LA/P/0037/2020/Fundacao para a Ciencia e Tecnologia/
GR  - UIDP/50025/2020/Fundacao para a Ciencia e Tecnologia/
GR  - UIDB/50025/2020/Fundacao para a Ciencia e Tecnologia/
GR  - CEECIND.03189.2020/Fundacao para a Ciencia e Tecnologia/
PT  - Journal Article
DEP - 20230106
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 9012-76-4 (Chitosan)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Humans
MH  - *Chitosan/chemistry
MH  - Durapatite/chemistry
MH  - Tissue Scaffolds/chemistry
MH  - Bone Regeneration
MH  - Magnetic Iron Oxide Nanoparticles
MH  - *Hyperthermia, Induced
MH  - Magnetic Phenomena
MH  - Tissue Engineering/methods
PMC - PMC9863008
OTO - NOTNLM
OT  - 3D printing
OT  - additive manufacturing
OT  - bone regeneration
OT  - chitosan
OT  - magnetic hyperthermia
OT  - poly (vinyl alcohol)
OT  - superparamagnetic iron oxide nanoparticles
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/25 06:00
PMCR- 2023/01/06
CRDT- 2023/01/21 01:25
PHST- 2022/11/18 00:00 [received]
PHST- 2022/12/22 00:00 [revised]
PHST- 2022/12/29 00:00 [accepted]
PHST- 2023/01/21 01:25 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2023/01/06 00:00 [pmc-release]
AID - ijms24021128 [pii]
AID - ijms-24-01128 [pii]
AID - 10.3390/ijms24021128 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jan 6;24(2):1128. doi: 10.3390/ijms24021128.