PMID- 36675683
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 2075-4426 (Print)
IS  - 2075-4426 (Electronic)
IS  - 2075-4426 (Linking)
VI  - 13
IP  - 1
DP  - 2022 Dec 22
TI  - Advanced Strategy of Trophoblasts Retrieval and Isolation from the Cervix (TRIC): 
      Comparison of Two HLA-G Antibodies for Immunomagnetic Isolation of Trophoblasts.
LID - 10.3390/jpm13010022 [doi]
LID - 22
AB  - Trophoblasts retrieval and isolation from the cervix (TRIC) is a non-invasive 
      method which enables analysis of fetal genetic information from the extravillous 
      trophoblast cells (EVTs). The aim of this study was to compare the efficacy of 
      the HLA-G antibodies-G233 and 4H84-in isolating EVT cells and provide an 
      optimized protocol of TRIC. We analyzed EVTs from 23 pregnant women in between 5 
      to 20 weeks of gestation who underwent invasive prenatal testing. Two HLA-G 
      antibodies-G233 and 4H84-were used in a subgroup of 11 and 12 samples for 
      immunomagnetic isolation. Cells with beta-hCG expression were counted to compare the 
      rate of isolated trophoblast cells. The rate of beta-hCG positive cells was 
      significantly different between the G233 and the 4H84 by immunefluorescence 
      microscopy (p < 0.001). The percentage of beta-hCG expressing cells in G233 and 4H84 
      groups were 62.4 +/- 8.24% and 82.6 +/- 7.1%, respectively (p < 0.001). The average 
      fetal cell positive rate was 14.1 +/- 3.78 in the G233 group while it was 25.8 +/- 
      3.9 in the 4H84 group by fluorescence in situ hybridization (FISH) (p = 0.011). 
      Immunoisolation of trophoblast cells using 4H84 HLA-G antibody was more efficient 
      in capturing EVT cells than using G233 for successful clinical application of 
      TRIC.
FAU - Hong, Kirim
AU  - Hong K
AUID- ORCID: 0000-0001-8258-9696
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA 
      University, Seoul 06125, Republic of Korea.
FAU - Jang, Hee Yeon
AU  - Jang HY
AUID- ORCID: 0000-0003-4127-4748
AD  - Department of Biomedical Science, College of Life Science, CHA University, 
      Seongnam 13488, Republic of Korea.
FAU - Shim, Sung Han
AU  - Shim SH
AD  - Department of Biomedical Science, College of Life Science, CHA University, 
      Seongnam 13488, Republic of Korea.
FAU - Cho, Hee Young
AU  - Cho HY
AUID- ORCID: 0000-0001-7064-5056
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA 
      University, Seoul 06125, Republic of Korea.
FAU - Cha, Dong Hyun
AU  - Cha DH
AUID- ORCID: 0000-0003-0722-1714
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA 
      University, Seoul 06125, Republic of Korea.
LA  - eng
GR  - HI21C1552/Public health technology research fund by national ministry of health 
      and welfare./
PT  - Journal Article
DEP - 20221222
PL  - Switzerland
TA  - J Pers Med
JT  - Journal of personalized medicine
JID - 101602269
PMC - PMC9865439
OTO - NOTNLM
OT  - HLA-G antibody
OT  - extravillous trophoblast cells
OT  - immunoisolation
OT  - trophoblasts retrieval and isolation from the cervix
OT  - beta-hCG
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/22 06:01
PMCR- 2022/12/22
CRDT- 2023/01/21 01:32
PHST- 2022/10/21 00:00 [received]
PHST- 2022/12/01 00:00 [revised]
PHST- 2022/12/21 00:00 [accepted]
PHST- 2023/01/21 01:32 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/22 06:01 [medline]
PHST- 2022/12/22 00:00 [pmc-release]
AID - jpm13010022 [pii]
AID - jpm-13-00022 [pii]
AID - 10.3390/jpm13010022 [doi]
PST - epublish
SO  - J Pers Med. 2022 Dec 22;13(1):22. doi: 10.3390/jpm13010022.