PMID- 36678439
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 12
IP  - 1
DP  - 2023 Jan 5
TI  - HSP27 Interacts with Nonstructural Proteins of Porcine Reproductive and 
      Respiratory Syndrome Virus and Promotes Viral Replication.
LID - 10.3390/pathogens12010091 [doi]
LID - 91
AB  - Heat shock protein 27 (HSP27) is a multifunctional protein and belongs to the 
      small HSP family. It has been shown that HSP27 is involved in viral replication 
      as a cellular chaperone, but the function of HSP27 during porcine reproductive 
      and respiratory syndrome virus (PRRSV) infections remains unexplored. Here, we 
      found that PRRSV replication can induce HSP27 expression and phosphorylation in 
      vitro. HSP27 overexpression promoted PRRSV replication, whereas its knockdown 
      reduced PRRSV proliferation. Additionally, suppressing HSP27 phosphorylation 
      reduced PRRSV replication and the level of viral double-stranded RNA (dsRNA), a 
      marker of the viral replication and transcription complexes (RTCs). Furthermore, 
      HSP27 can interact with multiple viral nonstructural proteins (nsps), including 
      nsp1alpha, nsp1beta, nsp5, nsp9, nsp11 and nsp12. Suppressing the phosphorylation of 
      HSP27 almost completely disrupted its interaction with nsp1beta and nsp12. 
      Altogether, our study revealed that HSP27 plays an important role in PRRSV 
      replication.
FAU - Song, Chunhui
AU  - Song C
AD  - College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, 
      China.
FAU - Liu, Hanze
AU  - Liu H
AD  - China Animal Health and Epidemiology Center, Qingdao 266032, China.
FAU - Cao, Zhi
AU  - Cao Z
AD  - College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, 
      China.
FAU - Shan, Hu
AU  - Shan H
AD  - College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, 
      China.
FAU - Zhang, Qiaoya
AU  - Zhang Q
AUID- ORCID: 0000-0002-7712-7670
AD  - College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, 
      China.
LA  - eng
GR  - 31902259/National Natural Sciences Foundation of China/
GR  - 1119029/Research Foundation for Distinguished Scholars of Qingdao Agricultural 
      University/
PT  - Journal Article
DEP - 20230105
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC9860683
OTO - NOTNLM
OT  - heat shock protein 27 (HSP27)
OT  - nonstructural proteins
OT  - porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - viral replication
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/22 06:01
PMCR- 2023/01/05
CRDT- 2023/01/21 01:48
PHST- 2022/11/29 00:00 [received]
PHST- 2022/12/30 00:00 [revised]
PHST- 2023/01/03 00:00 [accepted]
PHST- 2023/01/21 01:48 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/22 06:01 [medline]
PHST- 2023/01/05 00:00 [pmc-release]
AID - pathogens12010091 [pii]
AID - pathogens-12-00091 [pii]
AID - 10.3390/pathogens12010091 [doi]
PST - epublish
SO  - Pathogens. 2023 Jan 5;12(1):91. doi: 10.3390/pathogens12010091.