PMID- 36679445
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20231213
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 23
IP  - 2
DP  - 2023 Jan 6
TI  - A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of 
      Glyphosate in Tap Water.
LID - 10.3390/s23020649 [doi]
LID - 649
AB  - Glyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine 
      postemergence herbicide registered for many food and nonfood fields. Herein, we 
      developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads 
      incubated with NH2-polyA and then hybridized with polyT-glyphosate aptamer and 
      complementary DNA. Afterwards, a quantitative detection method based on qPCR was 
      established. When the glyphosate aptamer on Mbs@dsDNA specifically recognizes 
      glyphosate, complementary DNA is released and then enters the qPCR signal 
      amplification process. The linear range of the method was 0.6 mumol/L-30 mmol/L 
      and the detection limit was set at 0.6 mumol/L. The recoveries in tap water ranged 
      from 103.4 to 104.9% and the relative standard deviations (RSDs) were <1%. The 
      aptamer proposed in this study has good potential for recognizing glyphosate. The 
      detection method combined with qPCR might have good application prospects in 
      detecting and supervising other pesticide residues.
FAU - Shao, Yong
AU  - Shao Y
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
FAU - Tian, Run
AU  - Tian R
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
FAU - Duan, Jiaqi
AU  - Duan J
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - Wang, Miao
AU  - Wang M
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
FAU - Cao, Jing
AU  - Cao J
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
FAU - Cao, Zhen
AU  - Cao Z
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
FAU - Li, Guangyue
AU  - Li G
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - Jin, Fen
AU  - Jin F
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
AUID- ORCID: 0000-0001-6596-7907
AD  - State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy 
      of Sciences, Qilu University of Technology, Jinan 250353, China.
AD  - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 
      Giza 12211, Egypt.
AD  - Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240 
      Erzurum, Turkey.
FAU - She, Yongxin
AU  - She Y
AD  - Institute of Quality Standardization & Testing Technology for Agro-Products, 
      Chinese Academy of Agricultural Sciences, Beijing 100193, China.
AD  - Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture 
      and Rural Areas, Beijing 100081, China.
LA  - eng
GR  - No.Y2021XK21/Central Public-interest Scientific Institution Basal Research Fund/
GR  - CAAS-ZDRW202011/Agricultural Science and Technology Innovation Program of CAAS/
GR  - No.32072313/National Natural Science Foundation of China/
GR  - No.31772071/National Natural Science Foundation of China/
GR  - CARS-05-05A-03/China Agriculture Research System/
PT  - Journal Article
DEP - 20230106
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (DNA, Complementary)
RN  - 9007-49-2 (DNA)
RN  - 0 (Coloring Agents)
RN  - 0 (Aptamers, Nucleotide)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - DNA, Complementary
MH  - *DNA/chemistry
MH  - Coloring Agents
MH  - *Aptamers, Nucleotide/chemistry
MH  - Water
MH  - Glyphosate
PMC - PMC9863111
OTO - NOTNLM
OT  - SYBR Green I
OT  - aptamer
OT  - glyphosate
OT  - qPCR
OT  - sensor
COIS- The authors declare no conflict of interest.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/25 06:00
PMCR- 2023/01/06
CRDT- 2023/01/21 01:54
PHST- 2022/11/30 00:00 [received]
PHST- 2022/12/30 00:00 [revised]
PHST- 2023/01/01 00:00 [accepted]
PHST- 2023/01/21 01:54 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2023/01/06 00:00 [pmc-release]
AID - s23020649 [pii]
AID - sensors-23-00649 [pii]
AID - 10.3390/s23020649 [doi]
PST - epublish
SO  - Sensors (Basel). 2023 Jan 6;23(2):649. doi: 10.3390/s23020649.