PMID- 36680090
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20231116
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 15
IP  - 1
DP  - 2022 Dec 23
TI  - The Indirect ELISA and Monoclonal Antibody against African Swine Fever Virus p17 
      Revealed Efficient Detection and Application Prospects.
LID - 10.3390/v15010050 [doi]
LID - 50
AB  - Since 2018, the outbreak and prevalence of the African swine fever virus (ASFV) 
      in China have caused huge economic losses. Less virulent ASFVs emerged in 2020, 
      which led to difficulties and challenges for early diagnosis and control of 
      African swine fever (ASF) in China. An effective method of monitoring ASFV 
      antibodies and specific antibodies against ASFV to promote the development of 
      prevention techniques are urgently needed. In the present study, ASFV p17 was 
      successfully expressed in CHO cells using a suspension culture system. An 
      indirect enzyme-linked immunosorbent assay (ELISA) based on purified p17 was 
      established and optimized. The monoclonal antibody (mAb) against p17 recognized a 
      conservative linear epitope ((3)TETSPLLSH(11)) and exhibited specific reactivity, 
      which was conducive to the identification of recombinant porcine reproductive and 
      respiratory syndrome virus (PRRSV) expressing p17. The ELISA method efficiently 
      detected clinical ASFV infection and effectively monitored the antibody levels in 
      vivo after recombinant PRRSV live vector virus expressing p17 vaccination. 
      Overall, the determination of the conserved linear epitope of p17 would 
      contribute to the in-depth exploration of the biological function of the ASFV 
      antigen protein. The indirect ELISA method and mAb against ASFV p17 revealed 
      efficient detection and promising application prospects, making them ideal for 
      epidemiological surveillance and vaccine research on ASF.
FAU - Li, Liwei
AU  - Li L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Qiao, Sina
AU  - Qiao S
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, 
      China.
FAU - Li, Guoxin
AU  - Li G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Tong, Wu
AU  - Tong W
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Dong, Shishan
AU  - Dong S
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, 
      China.
FAU - Liu, Jiachen
AU  - Liu J
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Guo, Ziqiang
AU  - Guo Z
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Zheng, Haihong
AU  - Zheng H
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Zhao, Ran
AU  - Zhao R
AD  - Xiamen Center for Animal Disease Control and Prevention, Xiamen 361009, China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
AD  - Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal 
      Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China.
FAU - Gao, Fei
AU  - Gao F
AUID- ORCID: 0000-0003-2291-8712
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
AD  - Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal 
      Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221223
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Viral Proteins)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Swine
MH  - Animals
MH  - Cricetinae
MH  - *African Swine Fever Virus
MH  - *African Swine Fever/diagnosis/prevention & control/epidemiology
MH  - Viral Proteins
MH  - Antibodies, Monoclonal
MH  - Cricetulus
MH  - Vaccines, Synthetic
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Antibodies, Viral
PMC - PMC9865993
OTO - NOTNLM
OT  - ASFV p17
OT  - CHO cells
OT  - epitope
OT  - indirect ELISA
OT  - recombinant PRRSV
COIS- The authors declare that they have no conflict of interest associated with this 
      study.
EDAT- 2023/01/22 06:00
MHDA- 2023/01/25 06:00
PMCR- 2022/12/23
CRDT- 2023/01/21 01:58
PHST- 2022/12/09 00:00 [received]
PHST- 2022/12/20 00:00 [revised]
PHST- 2022/12/21 00:00 [accepted]
PHST- 2023/01/21 01:58 [entrez]
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/12/23 00:00 [pmc-release]
AID - v15010050 [pii]
AID - viruses-15-00050 [pii]
AID - 10.3390/v15010050 [doi]
PST - epublish
SO  - Viruses. 2022 Dec 23;15(1):50. doi: 10.3390/v15010050.