PMID- 36680742
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230202
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
VI  - 32
IP  - 1
DP  - 2023 Jan
TI  - Serological proteome analysis identifies crustacean myosin heavy chain type 1 
      protein and house dust mite Der p 14 as cross-reacting allergens.
PG  - 107-112
LID - 10.17219/acem/158773 [doi]
AB  - BACKGROUND: Allergies to house dust mite (HDM) and to crustaceans are clinically 
      and pathogenically linked. Several homologous allergenic proteins have been 
      identified, among which tropomyosin is the prototype, expressing epitopes endowed 
      with variable levels of immunoglobulin E (IgE) cross-reactivity. 
      Component-resolved diagnosis (CRD) does not allow a thorough characterization of 
      all relevant IgE reactivities to these allergen sources. OBJECTIVES: We studied 1 
      patient allergic to shrimp with positive skin prick test to HDM and negative 
      scores for IgE to HDM allergen components routinely used in CRD (group 1 and 2 
      allergens, Der p 23 and tropomyosin). MATERIAL AND METHODS: In order to identify 
      the allergen(s) involved in IgE reactivity, we used serological proteome analysis 
      (SERPA), which utilizes two-dimensional gel electrophoresis (2DE), immunoblotting 
      and mass spectrometry (MS). The identified allergenic proteins were tested with 
      sera from 20 crustacean-allergic patients and 19 grass-allergic patients serving 
      as controls. RESULTS: Der p 14 and myosin heavy chain type 1 (MHC1) were 
      identified as the components recognized by patient's IgE in the proteome of 
      Dermatophagoides pteronyssinus and Penaeus monodon, respectively. The MHC1 
      protein shows about 30% sequence identity with Der p 14 in specific domains, and 
      cross-reactivity against epitopes shared by the 2 proteins was demonstrated by 
      reduced reactivity to shrimp extract following pre-incubation with Der p 14. 
      Serum IgE from 5 out of 20 patients allergic to crustaceans reacted with MHC1, 
      compared to none among 19 controls (p < 0.05). CONCLUSION: We identified MHC1 as 
      a relevant allergic component in the proteome of Penaeus monodon, the prototypic 
      allergen source used in diagnosis of allergy to crustaceans. Our data demonstrate 
      MHC1 cross-reactivity between MHC1 and Der p 14 from Dermatophagoides 
      pteronyssinus.
FAU - Conti, Antonio
AU  - Conti A
AD  - Proteome Biochemistry, COSR-Centre for Omics Sciences, IRCCS-San Raffaele 
      Hospital, San Raffaele Scientific Institute, Milan, Italy.
FAU - Alqassir, Noor
AU  - Alqassir N
AD  - Proteome Biochemistry, COSR-Centre for Omics Sciences, IRCCS-San Raffaele 
      Hospital, San Raffaele Scientific Institute, Milan, Italy.
FAU - Breda, Daniela
AU  - Breda D
AD  - Laboratory of Cellular and Molecular Allergology, Division of Immunology, 
      Transplantation and Infectious Diseases, San Raffaele Scientific Institute, 
      Milan, Italy.
FAU - Zanardi, Alan
AU  - Zanardi A
AD  - Proteome Biochemistry, COSR-Centre for Omics Sciences, IRCCS-San Raffaele 
      Hospital, San Raffaele Scientific Institute, Milan, Italy.
FAU - Alessio, Massimo
AU  - Alessio M
AD  - Proteome Biochemistry, COSR-Centre for Omics Sciences, IRCCS-San Raffaele 
      Hospital, San Raffaele Scientific Institute, Milan, Italy.
FAU - Burastero, Samuele E
AU  - Burastero SE
AD  - Laboratory of Cellular and Molecular Allergology, Division of Immunology, 
      Transplantation and Infectious Diseases, San Raffaele Scientific Institute, 
      Milan, Italy.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
RN  - 0 (Proteome)
RN  - 0 (Tropomyosin)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Allergens
MH  - Epitopes/chemistry
MH  - *Hypersensitivity
MH  - Immunoglobulin E
MH  - Myosin Heavy Chains
MH  - Proteome
MH  - Pyroglyphidae
MH  - Tropomyosin/chemistry
MH  - Shellfish Hypersensitivity
MH  - Cross Reactions
OTO - NOTNLM
OT  - Dermatophagoides
OT  - allergens
OT  - case report
OT  - immunoglobulin E
OT  - proteomics
EDAT- 2023/01/22 06:00
MHDA- 2023/02/02 06:00
CRDT- 2023/01/21 11:32
PHST- 2023/01/22 06:00 [pubmed]
PHST- 2023/02/02 06:00 [medline]
PHST- 2023/01/21 11:32 [entrez]
AID - 10.17219/acem/158773 [doi]
PST - ppublish
SO  - Adv Clin Exp Med. 2023 Jan;32(1):107-112. doi: 10.17219/acem/158773.