PMID- 3668260
OWN - NLM
STAT- MEDLINE
DCOM- 19871209
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 103
IP  - 2
DP  - 1987 Nov 5
TI  - Composition of IgA immune complexes precipitated with polyethylene glycol. A 
      model for isolation and analysis of immune complexes.
PG  - 239-45
AB  - Covalently cross-linked large and intermediate-sized IgA oligomers, prepared with 
      IgA anti-dinitrophenyl (DNP) and bis-DNP-pimelic acid ester, were used to examine 
      the ability of different concentrations (3.5%, 5% and 7%, w/v) of polyethylene 
      glycol (PEG) to precipitate IgA immune complexes (IgA-IC). The size of the IgA-IC 
      precipitated with PEG was determined by gradient polyacrylamide gel 
      electrophoresis and quantitative autoradiography. The standard concentration of 
      3.5% PEG precipitated only a minor fraction (20%) of the IgA-IC. In contrast, 5% 
      and 7% PEG precipitated 45% and 79% of the complexes, respectively. To test the 
      influence of the antigen on the PEG assay. IgA-IC prepared with IgA anti-DNP and 
      DNP conjugates of either bovine serum albumin or Ficoll were also used. 
      Approximately 38% of these IgA-IC were precipitated with 3.5%, PEG. By 
      comparison, the concentration of 5% and 7% PEG precipitated 60% and 76% of the 
      IgA-IC, respectively. Distilled water rather than the standard borate-buffered 
      saline was shown to be the optimal solvent for resolubilization of the PEG 
      precipitates. Serum samples from 22 IgA nephropathy patients and 12 normal donors 
      were tested with 3.5%, 5% and 7% PEG. Only the 7% PEG assay showed a significant 
      difference between patients and controls (P less than 0.001) in the IgA levels of 
      precipitates. Thus, the use of 7% PEG is recommended for the detection, isolation 
      and analysis of large- and intermediate-sized IgA-IC.
FAU - Imai, H
AU  - Imai H
AD  - Department of Pathology and Laboratory Medicine, University of Texas Medical 
      School, Houston 77225.
FAU - Chen, A
AU  - Chen A
FAU - Wyatt, R J
AU  - Wyatt RJ
FAU - Rifai, A
AU  - Rifai A
LA  - eng
GR  - AM 32379/AM/NIADDK NIH HHS/United States
GR  - RR00211/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Immunoglobulin A)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Antigen-Antibody Complex/*analysis
MH  - Chemical Precipitation
MH  - Glomerulonephritis, IGA/immunology
MH  - Humans
MH  - Immunoglobulin A/*analysis
MH  - Molecular Weight
MH  - Polyethylene Glycols
MH  - Solubility
MH  - Structure-Activity Relationship
EDAT- 1987/11/05 00:00
MHDA- 1987/11/05 00:01
CRDT- 1987/11/05 00:00
PHST- 1987/11/05 00:00 [pubmed]
PHST- 1987/11/05 00:01 [medline]
PHST- 1987/11/05 00:00 [entrez]
AID - 0022-1759(87)90295-X [pii]
AID - 10.1016/0022-1759(87)90295-x [doi]
PST - ppublish
SO  - J Immunol Methods. 1987 Nov 5;103(2):239-45. doi: 10.1016/0022-1759(87)90295-x.