PMID- 36684388
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2023
DP  - 2023
TI  - Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes 
      Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the 
      alpha2beta1/FAK/PI3K/AKT Signaling Pathway In Vitro.
PG  - 2915826
LID - 10.1155/2023/2915826 [doi]
LID - 2915826
AB  - BACKGROUND: Tendon injuries are common clinical disorders. Due to the limited 
      regeneration ability of tendons, tissue engineering technology is often used as 
      an adjuvant treatment. This study explored the molecular pathways underlying 
      micropattern SF film-regulated TSPC propensity and their repairing effects to 
      highlight the application value of micropattern SF films. METHODS: First, we 
      characterized the physical properties of the micropattern SF films and explored 
      their repairing effects on the injured tendons in vivo. Then, we seeded TSPCs on 
      SF films in vitro and determined the micropattern SF film-induced gene expression 
      and activation of signaling pathways in TSPCs through high-throughput RNA 
      sequencing and proteomics assays. RESULTS: The results of in vivo studies 
      suggested that micropattern SF films can promote remodeling of the injured 
      tendon. In addition, immunohistochemistry (IHC) results showed that tendon marker 
      genes were significantly increased in the micropattern SF film repair group. 
      Transcriptomic and proteomic analyses demonstrated that micropattern SF 
      film-induced genes and proteins in TSPCs were mainly enriched in the focal 
      adhesion kinase (FAK)/actin and phosphoinositide 3-kinase (PI3K)/AKT pathways. 
      Western blot analysis showed that the expression of integrins alpha2beta1, tenascin-C 
      (TNC), and tenomodulin (TNMD) and the phosphorylation of AKT were significantly 
      increased in the micropattern SF film group, which could be abrogated by applying 
      PI3K/AKT inhibitors. CONCLUSION: Micropattern SF films modified by water 
      annealing can promote remodeling of the injured tendon in vivo and regulate the 
      tendon differentiation of TSPCs through the alpha2beta1/FAK/PI3K/AKT signaling pathway 
      in vitro. Therefore, they have great medical value in tendon repair.
CI  - Copyright (c) 2023 Kang Lu et al.
FAU - Lu, Kang
AU  - Lu K
AD  - Department of Orthopedics-Spine Surgery Center, the Second Affiliated Hospital, 
      Chongqing Medical University, Chongqing, China.
FAU - Tang, Hong
AU  - Tang H
AUID- ORCID: 0000-0003-0011-6575
AD  - Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, 
      Burn, and Combined Injury, Southwest Hospital, Army Medical University (Third 
      Military Medical University), Chongqing, China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Department of Orthopedics-Spine Surgery Center, the Second Affiliated Hospital, 
      Chongqing Medical University, Chongqing, China.
FAU - Wang, Liyuan
AU  - Wang L
AD  - Department of Orthopedics-Spine Surgery Center, the Second Affiliated Hospital, 
      Chongqing Medical University, Chongqing, China.
FAU - Zhou, Mei
AU  - Zhou M
AUID- ORCID: 0000-0002-6481-2206
AD  - Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, 
      Burn, and Combined Injury, Southwest Hospital, Army Medical University (Third 
      Military Medical University), Chongqing, China.
FAU - He, Gang
AU  - He G
AUID- ORCID: 0000-0001-9806-4069
AD  - Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, 
      Burn, and Combined Injury, Southwest Hospital, Army Medical University (Third 
      Military Medical University), Chongqing, China.
FAU - Lu, Hao
AU  - Lu H
AD  - State Key Laboratory of New Materials Composite Technology, Wuhan University of 
      Technology, Wuhan, Hubei, China.
FAU - Tang, Chuyue
AU  - Tang C
AD  - Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, 
      Burn, and Combined Injury, Southwest Hospital, Army Medical University (Third 
      Military Medical University), Chongqing, China.
FAU - Chen, Wan
AU  - Chen W
AD  - Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, 
      Burn, and Combined Injury, Southwest Hospital, Army Medical University (Third 
      Military Medical University), Chongqing, China.
FAU - Ma, Xiaoqing
AU  - Ma X
AUID- ORCID: 0000-0001-6949-529X
AD  - School of Chemistry and Chemical Engineering, Yangtze Normal University, 
      Chongqing, China.
FAU - Tang, Kanglai
AU  - Tang K
AUID- ORCID: 0000-0002-0370-106X
AD  - Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, 
      Burn, and Combined Injury, Southwest Hospital, Army Medical University (Third 
      Military Medical University), Chongqing, China.
FAU - Deng, Zhongliang
AU  - Deng Z
AUID- ORCID: 0000-0001-5330-3062
AD  - Department of Orthopedics-Spine Surgery Center, the Second Affiliated Hospital, 
      Chongqing Medical University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20230113
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC9859702
COIS- The authors declare no conflicts of interest.
EDAT- 2023/01/24 06:00
MHDA- 2023/01/24 06:01
PMCR- 2023/01/13
CRDT- 2023/01/23 04:22
PHST- 2022/06/23 00:00 [received]
PHST- 2022/11/23 00:00 [revised]
PHST- 2022/12/08 00:00 [accepted]
PHST- 2023/01/23 04:22 [entrez]
PHST- 2023/01/24 06:00 [pubmed]
PHST- 2023/01/24 06:01 [medline]
PHST- 2023/01/13 00:00 [pmc-release]
AID - 10.1155/2023/2915826 [doi]
PST - epublish
SO  - Stem Cells Int. 2023 Jan 13;2023:2915826. doi: 10.1155/2023/2915826. eCollection 
      2023.