PMID- 36686454
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20240226
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - Methanol extract of Inonotus obliquus improves type 2 diabetes mellitus through 
      modifying intestinal flora.
PG  - 1103972
LID - 10.3389/fendo.2022.1103972 [doi]
LID - 1103972
AB  - Type 2 diabetes mellitus (T2DM) poses a significant risk to human health. 
      Previous research demonstrated that Inonotus obliquus possesses good 
      hypolipidemic, anti-inflammatory, and anti-tumor properties. In this research, we 
      aim to investigate the potential treatment outcomes of Inonotus obliquus for T2DM 
      and discuss its favourable influences on the intestinal flora. The chemical 
      composition of Inonotus obliquus methanol extracts (IO) was analyzed by 
      ultra-high-performance liquid chromatography-Q extractive-mass spectrometry. IO 
      significantly improved the blood glucose level, blood lipid level, and 
      inflammatory factor level in T2DM mice, and effectively alleviated the 
      morphological changes of colon, liver and renal. Acetic acid, propionic acid, and 
      butyric acid levels in the feces of the IO group were restored. 16S rRNA gene 
      sequencing revealed that the intestinal flora composition of mice in the IO group 
      was significantly modulated. Inonotus obliquus showed significant hypoglycemic 
      and hypolipidemic effects with evident anti-inflammatory activity and improved 
      the morphological structure of various organs and cells. Inonotus obliquus 
      increased the levels of short-chain fatty acids in the environment by increasing 
      the population of certain bacteria that produce acid, such as Alistipes and 
      Akkermansia, which are beneficial to improve intestinal flora disorders and 
      maintain intestinal flora homeostasis. Meanwhile, Inonotus obliquus further 
      alleviated T2DM symptoms in db/db mice by down-regulating the high number of 
      microorganisms that are dangerous, such as Proteobacteria and 
      Rikenellaceae_RC9_gut_group and up-regulating the abundance of beneficial 
      bacteria such as Odoribacter and Rikenella. Therefore, this study provides a new 
      perspective for the treatment of T2DM by demonstrating that drug and food 
      homologous active substances could relieve inflammation via regulating intestinal 
      flora.
CI  - Copyright (c) 2023 Ye, Wu, Xu, Cen, Ni, Chen, Zheng and Liu.
FAU - Ye, Xuewei
AU  - Ye X
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Wu, Kefei
AU  - Wu K
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Xu, Langyu
AU  - Xu L
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Cen, Yingxin
AU  - Cen Y
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Ni, Jiahui
AU  - Ni J
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Chen, Junyao
AU  - Chen J
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Zheng, Wenxin
AU  - Zheng W
AD  - Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang 
      Province, Department of Basic Medical Sciences, Shulan International Medical 
      College, Zhejiang Shuren University, Hangzhou, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural 
      Sciences, Hangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230106
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - Y4S76JWI15 (Methanol)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Plant Extracts)
RN  - 0 (Anti-Inflammatory Agents)
RN  - Inonotus obliquus
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - Methanol
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - *Gastrointestinal Microbiome
MH  - RNA, Ribosomal, 16S
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - Anti-Inflammatory Agents/therapeutic use
PMC - PMC9852891
OTO - NOTNLM
OT  - Inonotus obliquus
OT  - SCFAs
OT  - T2DM
OT  - db/db mice
OT  - intestinal flora
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/24 06:00
MHDA- 2023/01/25 06:00
PMCR- 2022/01/01
CRDT- 2023/01/23 04:50
PHST- 2022/11/21 00:00 [received]
PHST- 2022/12/19 00:00 [accepted]
PHST- 2023/01/23 04:50 [entrez]
PHST- 2023/01/24 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.1103972 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2023 Jan 6;13:1103972. doi: 
      10.3389/fendo.2022.1103972. eCollection 2022.