PMID- 36688616
OWN - NLM
STAT- MEDLINE
DCOM- 20230223
LR  - 20230501
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 148
IP  - 4
DP  - 2023 Feb 13
TI  - A comparative study of aptamer isolation by conventional and microfluidic 
      strategies.
PG  - 787-798
LID - 10.1039/d2an01767a [doi]
AB  - Aptamers are single-stranded oligonucleotide molecules that bind with high 
      affinity and specificity to a wide range of target molecules. The method of 
      systematic evolution of ligands by exponential enrichment (SELEX) plays an 
      essential role in the isolation of aptamers from a randomized oligonucleotide 
      library. To date, significant modifications and improvements of the SELEX process 
      have been achieved, engendering various forms of SELEX from conventional SELEX to 
      microfluidics-based full-chip SELEX. While full-chip SELEX is generally 
      considered advantageous over conventional SELEX, there has not yet been a 
      conclusive comparison between the methods. Herein, we present a comparative study 
      of three SELEX strategies for aptamer isolation, including those using 
      conventional agarose bead-based partitioning, microfluidic affinity selection, 
      and fully integrated microfluidic affinity selection and PCR amplification. Using 
      immunoglobulin E (IgE) as a model target molecule, we compare these strategies in 
      terms of the time and cost for each step of the SELEX process including affinity 
      selection, amplification, and oligonucleotide conditioning. Target-binding 
      oligonucleotides in the enriched pools are sequenced and compared to assess the 
      relative efficacy of the SELEX strategies. We show that the microfluidic 
      strategies are more time- and cost-efficient than conventional SELEX.
FAU - Meng, Xin
AU  - Meng X
AUID- ORCID: 0000-0001-8146-3186
AD  - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, 
      USA. qlin@columbia.edu.
FAU - Wen, Kechun
AU  - Wen K
AUID- ORCID: 0000-0002-4470-6692
AD  - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, 
      USA. qlin@columbia.edu.
FAU - Citartan, Marimuthu
AU  - Citartan M
AUID- ORCID: 0000-0001-5395-0040
AD  - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, 
      USA. qlin@columbia.edu.
AD  - Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 
      13200, Kepala Batas, Penang, Malaysia.
FAU - Lin, Qiao
AU  - Lin Q
AD  - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, 
      USA. qlin@columbia.edu.
LA  - eng
GR  - R01 EB032910/EB/NIBIB NIH HHS/United States
GR  - R01 GM138843/GM/NIGMS NIH HHS/United States
GR  - R21 CA261775/CA/NCI NIH HHS/United States
GR  - U19 AI067773/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20230213
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Ligands)
SB  - IM
MH  - *Aptamers, Nucleotide/genetics/chemistry
MH  - Base Sequence
MH  - Ligands
MH  - *Microfluidics/methods
MH  - Polymerase Chain Reaction
MH  - SELEX Aptamer Technique/methods
PMC - PMC10143297
MID - NIHMS1883539
COIS- Conflicts of interest There are no conflicts to declare.
EDAT- 2023/01/24 06:00
MHDA- 2023/02/16 06:00
PMCR- 2023/04/28
CRDT- 2023/01/23 08:33
PHST- 2023/01/24 06:00 [pubmed]
PHST- 2023/02/16 06:00 [medline]
PHST- 2023/01/23 08:33 [entrez]
PHST- 2023/04/28 00:00 [pmc-release]
AID - 10.1039/d2an01767a [doi]
PST - epublish
SO  - Analyst. 2023 Feb 13;148(4):787-798. doi: 10.1039/d2an01767a.