PMID- 36689051
OWN - NLM
STAT- MEDLINE
DCOM- 20230330
LR  - 20230330
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 50
IP  - 4
DP  - 2023 Apr
TI  - Silencing TAB182 inhibits cell EMT, migration and invasion by downregulating EGFR 
      in A549 NSCLC cells.
PG  - 3073-3083
LID - 10.1007/s11033-022-08176-5 [doi]
AB  - BACKGROUND: TAB182 is overexpressed in cancerous tissues and correlated with poor 
      overall survival in lung cancer patients. Mechanistically, TAB182 participates in 
      DNA damage repair and endows tumour cells with radio- and chemoresistance. 
      However, its role in non-small cell lung cancer (NSCLC) remains unclear. METHODS 
      AND RESULTS: Cells with stable TAB182 knockdown (KD) were generated using A549 
      NSCLC cells, and we demonstrated that depleting TAB182 inhibits cell EMT, 
      proliferation, colony formation, migration and invasion. Analysis of the TCGA 
      database showed a positive correlation between TAB182 and EGFR, a 
      well-established NSCLC oncoprotein. Then, we verified that silencing TAB182 
      decreases EGFR expression at both the mRNA and protein levels. Moreover, both 
      TAB182 and EGFR were reported to restore ionizing radiation (IR)-triggered DNA 
      damage. We validated that IR elevates the protein level of EGFR and that 
      silencing TAB182 can alleviate IR-induced EGFR upregulation. Furthermore, 
      overexpressing EGFR abrogates the inhibitory effects of TAB182 KD on EMT, 
      migration, and invasion in A549 cells. CONCLUSIONS: Our data demonstrated that 
      EGFR expression is regulated by TAB182 and downregulation of TAB182 has a novel 
      function to repress EMT, migration and invasion by decreasing EGFR, indicating 
      TAB182 could regulate the malignant progression of NSCLC.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Wang, Shaozheng
AU  - Wang S
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
FAU - Guo, Hejiang
AU  - Guo H
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
FAU - Jia, Jin
AU  - Jia J
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
AD  - School of Medicine, University of South China, Hengyang, 421001, China.
FAU - Zhang, Wen
AU  - Zhang W
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
AD  - School of Medicine, University of South China, Hengyang, 421001, China.
FAU - Gao, Shanshan
AU  - Gao S
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
FAU - Guan, Hua
AU  - Guan H
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
FAU - He, Huan
AU  - He H
AUID- ORCID: 0000-0001-8123-5765
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China. 
      hehuan@jlu.edu.cn.
AD  - NHC Key Laboratory of Radiobiology, School of Public Health, Jilin University, 
      Changchun, 130021, China. hehuan@jlu.edu.cn.
FAU - Zhou, Pingkun
AU  - Zhou P
AD  - Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for 
      Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, China. 
      zhoupk@bmi.ac.cn.
AD  - School of Medicine, University of South China, Hengyang, 421001, China. 
      zhoupk@bmi.ac.cn.
LA  - eng
GR  - 31870847/National Natural Science Foundation of China/
GR  - 32171238/National Natural Science Foundation of China/
GR  - 2021M693965/Postdoctoral Research Foundation of China/
PT  - Journal Article
DEP - 20230123
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - 0 (TNKS1BP1 protein, human)
SB  - IM
MH  - Humans
MH  - A549 Cells
MH  - *Carcinoma, Non-Small-Cell Lung/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - ErbB Receptors/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - *Lung Neoplasms/metabolism
OTO - NOTNLM
OT  - Cell invasion
OT  - Cell migration
OT  - EGFR
OT  - EMT
OT  - Lung cancer cells
OT  - TAB182
EDAT- 2023/01/24 06:00
MHDA- 2023/03/29 06:05
CRDT- 2023/01/23 11:17
PHST- 2022/09/07 00:00 [received]
PHST- 2022/12/06 00:00 [accepted]
PHST- 2023/03/29 06:05 [medline]
PHST- 2023/01/24 06:00 [pubmed]
PHST- 2023/01/23 11:17 [entrez]
AID - 10.1007/s11033-022-08176-5 [pii]
AID - 10.1007/s11033-022-08176-5 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2023 Apr;50(4):3073-3083. doi: 10.1007/s11033-022-08176-5. Epub 
      2023 Jan 23.