PMID- 36697993
OWN - NLM
STAT- MEDLINE
DCOM- 20230823
LR  - 20230823
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Linking)
VI  - 65
IP  - 9
DP  - 2023 Sep
TI  - CYTOR Promotes Proliferation of Lung Cancer Cell by Targeting miR-103a-3p to 
      Upregulate HMGB1.
PG  - 1528-1538
LID - 10.1007/s12033-023-00662-w [doi]
AB  - Lung cancer is one of the most dangerous malignant tumors to human health in the 
      world. Previous researches have shown that cytoskeleton regulator RNA (CYTOR), a 
      long noncoding RNA was involved in the occurrence and development of various 
      types of cancer. The aim of this study is to investigate the clinical 
      significance and biological function of CYTOR in lung cancer. Real-time 
      quantitative PCR was applied to detect the expression of CYTOR. The proliferation 
      of A549 and H1299 cells was analyzed by CCK8 assay. The luciferase reporter assay 
      and RNA pull-down assay were used to reveal the interactions between CYTOR and 
      its downstream targets. Western blot was used to detect the expression of 
      high-mobility group protein B1 (HMGB1). Here we found CYTOR was upregulated in 
      lung cancer tissues and cell lines. The proliferation of A549 and H1299 cells was 
      inhibited after CYTOR silencing. In addition, CYTOR could directly interact with 
      and negatively regulate miR-103a-3p, and miR-103a-3p inhibited cell proliferation 
      by targeting HMGB1. The CYTOR/miR-103a-3p/HMGB1 axis promoted lung cancer cell 
      proliferation. CYTOR sponges miR-103a-3p to promote the proliferation of lung 
      cancer cells through HMGB1. The CYTOR/miR-103a-3p/HMGB1 axis plays a critical 
      role in the progression of lung cancer.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Xu, Xinxin
AU  - Xu X
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nantong University, 
      Nantong, 226001, China.
FAU - Chen, Yi
AU  - Chen Y
AD  - Department of Clinical Laboratory Shanghai East Hospital, Tongji University, 
      Shanghai, 200123, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nantong University, 
      Nantong, 226001, China.
FAU - Cai, Hua
AU  - Cai H
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nantong University, 
      Nantong, 226001, China.
FAU - Shen, Pei
AU  - Shen P
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nantong University, 
      Nantong, 226001, China.
FAU - Peng, Jingjing
AU  - Peng J
AD  - Department of Laboratory Medicine, Nantong First People's Hospital, Nantong, 
      226001, China.
FAU - Liu, Hongli
AU  - Liu H
AD  - Department of Laboratory Medicine, Nantong Tumor Hospital, Nantong, 226001, 
      China.
FAU - Chen, Xiang
AU  - Chen X
AD  - Department of Laboratory Medicine, Nantong First People's Hospital, Nantong, 
      226001, China.
FAU - Chu, Fuying
AU  - Chu F
AUID- ORCID: 0000-0001-8400-396X
AD  - Department of Laboratory Medicine, Nantong First People's Hospital, Nantong, 
      226001, China. fychu19870817@163.com.
LA  - eng
GR  - JC2020070/Nantong Science and Technology Project/
GR  - MS220211001/Science and Technology Project of Nantong City/
PT  - Journal Article
DEP - 20230125
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (HMGB1 Protein)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Humans
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *HMGB1 Protein/genetics/metabolism
MH  - *Lung Neoplasms/genetics/pathology
MH  - *MicroRNAs/genetics/metabolism
OTO - NOTNLM
OT  - CYTOR
OT  - HMGB1
OT  - Lung cancer
OT  - Proliferation
OT  - miR-103a-3p
EDAT- 2023/01/26 06:00
MHDA- 2023/08/23 06:42
CRDT- 2023/01/25 23:50
PHST- 2022/10/07 00:00 [received]
PHST- 2023/01/11 00:00 [accepted]
PHST- 2023/08/23 06:42 [medline]
PHST- 2023/01/26 06:00 [pubmed]
PHST- 2023/01/25 23:50 [entrez]
AID - 10.1007/s12033-023-00662-w [pii]
AID - 10.1007/s12033-023-00662-w [doi]
PST - ppublish
SO  - Mol Biotechnol. 2023 Sep;65(9):1528-1538. doi: 10.1007/s12033-023-00662-w. Epub 
      2023 Jan 25.