PMID- 36699599
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Akkermansia deficiency and mucin depletion are implicated in intestinal barrier 
      dysfunction as earlier event in the development of inflammation in 
      interleukin-10-deficient mice.
PG  - 1083884
LID - 10.3389/fmicb.2022.1083884 [doi]
LID - 1083884
AB  - BACKGROUND: Dysbiosis and mucin depletion are related with intestinal barrier 
      dysfunction and seems to be an early pathophysiological event in inflammatory 
      bowel disease (IBD). The objective of this work is to study these parameters in 
      the natural history of colitis in IL-10 deficient mice (IL-10(-/-)). METHODS: 
      Wild type (WT) and IL-10(-/-). mice were followed until sacrifice at 3, 5, 10, 
      20, 57, and 70 weeks. Body weight, colonic weight/length ratio and in vivo 
      intestinal permeability were registered. Expression of inflammatory and adhesion 
      molecules in the colon was explored by qPCR as Mucin-2 (MUC-2) and molecules 
      involved in goblet cell maturation Interleukin-18 (IL-18) and WAP Four-Disulfide 
      Core Domain 2 (WFDC2), the endoplasmic reticulum stress markers X-box-binding 
      protein (Xbp-1) and Reticulon-4B (RTN-4B). Bacterial composition in feces and 
      colonic mucosa was determined by massive sequencing of the V3-V4 regions of 16S 
      rDNA gene. RESULTS: IL-10(-/-) mice showed histological inflammation at weeks 20 
      and 57, but most notably the intestinal permeability was significantly higher 
      from week 10. Concordantly, the number of goblet cells and expression of MUC-2, 
      IL-18, WFDC2 and Xbp-1 were significantly lower in KO from week 10. Nevertheless, 
      no significant differences were found in the mRNA expression of MUC-2 or Xbp-1 
      between both groups-derived colon organoids. Significant bacterial differences 
      began at week 5, being the Akkermansia deficiency in KO the most relevant result. 
      CONCLUSION: Gut microbiota alterations and mucin depletion are associated with 
      early intestinal barrier dysfunction and precede overt gut inflammation in this 
      animal model of IBD.
CI  - Copyright (c) 2023 Lopez-Cauce, Puerto, Garcia, Ponce-Alonso, Becerra-Aparicio, del 
      Campo, Peligros, Fernandez-Acenero, Gomez-Navarro, Lara, Miranda-Bautista, 
      Marin-Jimenez, Banares and Menchen.
FAU - Lopez-Cauce, Beatriz
AU  - Lopez-Cauce B
AD  - Servicio de Aparato Digestivo, Hospital General Universitario Gregorio Maranon, 
      Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBEREHD), Madrid, Spain.
AD  - Departamento de Microbiologia y Parasitologia Facultad de Farmacia, Universidad 
      Complutense, Madrid, Spain.
FAU - Puerto, Marta
AU  - Puerto M
AD  - Servicio de Aparato Digestivo, Hospital General Universitario Gregorio Maranon, 
      Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBEREHD), Madrid, Spain.
FAU - Garcia, Juan Jose
AU  - Garcia JJ
AD  - Departamento de Microbiologia y Parasitologia Facultad de Farmacia, Universidad 
      Complutense, Madrid, Spain.
FAU - Ponce-Alonso, Manuel
AU  - Ponce-Alonso M
AD  - Servicio de Microbiologia, Hospital Ramon y Cajal, CIBERINFEC, IRYCIS, Madrid, 
      Spain.
FAU - Becerra-Aparicio, Federico
AU  - Becerra-Aparicio F
AD  - Servicio de Microbiologia, Hospital Ramon y Cajal, CIBERINFEC, IRYCIS, Madrid, 
      Spain.
FAU - Del Campo, Rosa
AU  - Del Campo R
AD  - Servicio de Microbiologia, Hospital Ramon y Cajal, CIBERINFEC, IRYCIS, Madrid, 
      Spain.
FAU - Peligros, Isabel
AU  - Peligros I
AD  - Servicio de Anatomia Patologica, Hospital General Universitario Gregorio Maranon, 
      Madrid, Spain.
FAU - Fernandez-Acenero, Maria J
AU  - Fernandez-Acenero MJ
AD  - Servicio de Anatomia Patologica, Hospital General Universitario Gregorio Maranon, 
      Madrid, Spain.
FAU - Gomez-Navarro, Yesica
AU  - Gomez-Navarro Y
AD  - Servicio de Anatomia Patologica, Hospital General Universitario Gregorio Maranon, 
      Madrid, Spain.
FAU - Lara, Jose M
AU  - Lara JM
AD  - Servicio de Anatomia Patologica, Hospital General Universitario Gregorio Maranon, 
      Madrid, Spain.
FAU - Miranda-Bautista, Jose
AU  - Miranda-Bautista J
AD  - Servicio de Aparato Digestivo, Hospital General Universitario Gregorio Maranon, 
      Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBEREHD), Madrid, Spain.
FAU - Marin-Jimenez, Ignacio
AU  - Marin-Jimenez I
AD  - Servicio de Aparato Digestivo, Hospital General Universitario Gregorio Maranon, 
      Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBEREHD), Madrid, Spain.
FAU - Banares, Rafael
AU  - Banares R
AD  - Servicio de Aparato Digestivo, Hospital General Universitario Gregorio Maranon, 
      Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBEREHD), Madrid, Spain.
AD  - Departamento de Medicina, Facultad de Medicina, Universidad Complutense, Madrid, 
      Spain.
FAU - Menchen, Luis
AU  - Menchen L
AD  - Servicio de Aparato Digestivo, Hospital General Universitario Gregorio Maranon, 
      Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBEREHD), Madrid, Spain.
AD  - Departamento de Medicina, Facultad de Medicina, Universidad Complutense, Madrid, 
      Spain.
LA  - eng
PT  - Journal Article
DEP - 20230109
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9869054
OTO - NOTNLM
OT  - Akkermansia muciniphila
OT  - gut microbiome
OT  - inflammatory bowel disease
OT  - interleukin-10-deficient mice
OT  - intestinal barrier
OT  - mucin
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/27 06:00
MHDA- 2023/01/27 06:01
PMCR- 2023/01/09
CRDT- 2023/01/26 02:47
PHST- 2022/10/31 00:00 [received]
PHST- 2022/12/23 00:00 [accepted]
PHST- 2023/01/26 02:47 [entrez]
PHST- 2023/01/27 06:00 [pubmed]
PHST- 2023/01/27 06:01 [medline]
PHST- 2023/01/09 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.1083884 [doi]
PST - epublish
SO  - Front Microbiol. 2023 Jan 9;13:1083884. doi: 10.3389/fmicb.2022.1083884. 
      eCollection 2022.