PMID- 36699677
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 13
DP  - 2022
TI  - Effects of electroacupuncture on bladder dysfunction and the expression of 
      PACAP38 in a diabetic rat model.
PG  - 1008269
LID - 10.3389/fphys.2022.1008269 [doi]
LID - 1008269
AB  - Objective: To explore the effects and the possible mechanism of 
      electroacupuncture (EA) on diabetic bladder dysfunction (DBD) in 
      streptozotocin-high fat diet (STZ-HFD) induced type 2 diabetes mellitus (T2DM) 
      rats. Methods: The experiment was divided into Control, diabetic bladder 
      dysfunction, electroacupuncture, and Sham electroacupuncture group. After 8 weeks 
      of electroacupuncture intervention, the body mass, 24 h urine volume, 
      intraperitoneal glucose tolerance test (IPGTT), and urodynamics were detected. 
      After the wet weight of the bladder was detected, the hematoxylin-eosin (HE), 
      Masson's trichrome, and TUNEL were used to analyze histological changes. The 
      PACAP38 expressions in the bladder were detected by Real-time PCR and Western 
      blot. Results: Compared to the Control group, the bladder wet weight, 24 h urine 
      volume, blood glucose, maximum bladder capacity, bladder compliance, bladder wall 
      thickness, the smooth muscle/collagen ratio, and apoptosis rate of the diabetic 
      bladder dysfunction group were significantly increased. Moreover, the body mass 
      and leak point pressure were significantly reduced. Compared with the Sham 
      electroacupuncture group, the bladder wet weight, maximum bladder capacity, 
      bladder compliance, bladder wall thickness, and apoptosis rate of the 
      electroacupuncture group were significantly reduced. In contrast, the leak point 
      pressure was increased. The PACAP38 mRNA and PACAP38 protein expression of the 
      diabetic bladder dysfunction group were significantly lower than the Control 
      group, while electroacupuncture treatment could upregulate PACAP38 mRNA levels 
      and PACAP38 protein expression of diabetic bladder dysfunction model rats. 
      Conclusion: electroacupuncture could ameliorate bladder dysfunction in the 
      diabetic bladder dysfunction model rats by reversing bladder remodeling, which 
      might be mainly mediated by regulating the PACAP38 level.
CI  - Copyright (c) 2023 Han, Chen, Ha, Yang, Wang, Chen, Zhou, Long, Qiu and Chen.
FAU - Han, Xuke
AU  - Han X
AD  - College of Acupuncture and Massage, Shaanxi University of Chinese Medicine, 
      Xianyang, China.
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Chen, Yiding
AU  - Chen Y
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Ha, Lue
AU  - Ha L
AD  - College of Acupuncture and Massage, Shaanxi University of Chinese Medicine, 
      Xianyang, China.
FAU - Yang, Jiao
AU  - Yang J
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Wang, Fangzhou
AU  - Wang F
AD  - College of Acupuncture and Massage, Shaanxi University of Chinese Medicine, 
      Xianyang, China.
FAU - Chen, Huizhen
AU  - Chen H
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Zhou, Qian
AU  - Zhou Q
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Long, Cong
AU  - Long C
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Qiu, Xianliang
AU  - Qiu X
AD  - West China Second Hospital, Sichuan University, Chengdu, China.
FAU - Chen, Qiu
AU  - Chen Q
AD  - Department of Endocrinology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20230109
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC9868671
OTO - NOTNLM
OT  - cystometry
OT  - diabetic bladder dysfunction
OT  - electroacupuncture
OT  - pituitary adenylate cyclase activating polypeptide
OT  - type 2 diabetes mellitus rats
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/27 06:00
MHDA- 2023/01/27 06:01
PMCR- 2023/01/09
CRDT- 2023/01/26 02:48
PHST- 2022/08/02 00:00 [received]
PHST- 2022/12/21 00:00 [accepted]
PHST- 2023/01/26 02:48 [entrez]
PHST- 2023/01/27 06:00 [pubmed]
PHST- 2023/01/27 06:01 [medline]
PHST- 2023/01/09 00:00 [pmc-release]
AID - 1008269 [pii]
AID - 10.3389/fphys.2022.1008269 [doi]
PST - epublish
SO  - Front Physiol. 2023 Jan 9;13:1008269. doi: 10.3389/fphys.2022.1008269. 
      eCollection 2022.