PMID- 36700212
OWN - NLM
STAT- MEDLINE
DCOM- 20230130
LR  - 20230202
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - A highly efficient indirect ELISA and monoclonal antibody established against 
      African swine fever virus pK205R.
PG  - 1103166
LID - 10.3389/fimmu.2022.1103166 [doi]
LID - 1103166
AB  - African swine fever (ASF) is a contagious infectious disease with high lethality 
      which continuously threatens the global pig industry causing huge economic 
      losses. Currently, there are no commercially available vaccines or antiviral 
      drugs that can effectively control ASF. The pathogen of ASF, ASF virus (ASFV) is 
      a double-stranded DNA virus with a genome ranging from 170 to 193 kb and 151 to 
      167 open reading frames in various strains, which encodes 150-200 proteins. An 
      effective method of monitoring ASFV antibodies, and specific antibodies against 
      ASFV to promote the development of prevention techniques are urgently needed. In 
      the present study, pK205R of ASFV was successfully expressed in mammalian cells 
      using a suspension culture system. An indirect enzyme-linked immunosorbent assay 
      (ELISA) based on the purified pK205R was established and optimized. The 
      monoclonal antibody (mAb) against pK205R recognized a conservative linear epitope 
      ((2)VEPREQFFQDLLSAV(16)) and exhibited specific reactivity, which was conducive 
      to the identification of the recombinant porcine reproductive and respiratory 
      syndrome virus (PRRSV) expressing pK205R. The ELISA method efficiently detected 
      clinical ASFV infection and revealed good application prospects in monitoring the 
      antibody level in vivo for recombinant PRRSV live vector virus expressing the 
      ASFV antigen protein. The determination of the conserved linear epitope of pK205R 
      would contribute to further research on the structural biology and function of 
      pK205R. The indirect ELISA method and mAb against ASFV pK205R revealed efficient 
      detection and promising application prospects, making them ideal for 
      epidemiological surveillance and vaccine research on ASF.
CI  - Copyright (c) 2023 Li, Qiao, Liu, Zhou, Tong, Dong, Liu, Jiang, Guo, Zheng, Zhao, 
      Tong, Li and Gao.
FAU - Li, Liwei
AU  - Li L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Qiao, Sina
AU  - Qiao S
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
FAU - Liu, Jiachen
AU  - Liu J
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Tong, Wu
AU  - Tong W
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Dong, Shishan
AU  - Dong S
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
FAU - Liu, Changlong
AU  - Liu C
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Jiang, Yifeng
AU  - Jiang Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Guo, Ziqiang
AU  - Guo Z
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Zheng, Haihong
AU  - Zheng H
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Zhao, Ran
AU  - Zhao R
AD  - Xiamen Center for Animal Disease Control and Prevention, Xiamen, China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
FAU - Li, Guoxin
AU  - Li G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
AD  - Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal 
      Infectious Disease and Zoonose, Yangzhou University, Yangzhou, China.
FAU - Gao, Fei
AU  - Gao F
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, China.
AD  - Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal 
      Infectious Disease and Zoonose, Yangzhou University, Yangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230109
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - *African Swine Fever
MH  - *African Swine Fever Virus
MH  - *Antibodies, Monoclonal/immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Swine
MH  - Viral Proteins
PMC - PMC9868132
OTO - NOTNLM
OT  - 293i cells
OT  - ASFV pK205R
OT  - epitope
OT  - indirect ELISA
OT  - recombinant PRRSV
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/27 06:00
MHDA- 2023/01/28 06:00
PMCR- 2022/01/01
CRDT- 2023/01/26 02:55
PHST- 2022/11/20 00:00 [received]
PHST- 2022/12/21 00:00 [accepted]
PHST- 2023/01/26 02:55 [entrez]
PHST- 2023/01/27 06:00 [pubmed]
PHST- 2023/01/28 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.1103166 [doi]
PST - epublish
SO  - Front Immunol. 2023 Jan 9;13:1103166. doi: 10.3389/fimmu.2022.1103166. 
      eCollection 2022.