PMID- 36703209
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 1754-1611 (Print)
IS  - 1754-1611 (Electronic)
IS  - 1754-1611 (Linking)
VI  - 17
IP  - 1
DP  - 2023 Jan 26
TI  - The synergistic and enhancive effects of IL-6 and M-CSF to expand and 
      differentiate functional dendritic cells from human monocytes under serum-free 
      condition.
PG  - 6
LID - 10.1186/s13036-023-00325-z [doi]
LID - 6
AB  - BACKGROUND: Dendritic cells (DCs) are differentiated from monocytes, and have a 
      strong ability to perform phagocytosis, present antigens and activate T cell 
      immune response. Therefore, DCs are one of the key factors in fighting cancer in 
      immunotherapy, and it is an important issue to develop a serum-free system for DC 
      differentiation and expansion in vitro for clinical application. RESULTS: In this 
      study, IL-6 and M-CSF were determined and a concentration combination of 
      cytokines was optimized to develop an optimal DC serum-free differentiation 
      medium (SF-DC Optimal) that can effectively differentiate CD14(+) monocytes into 
      CD40(+)CD209(+) DCs. After differentiation, the morphology, growth kinetics, 
      surface antigen expression, phagocytosis ability, cytokine secretion, mixed 
      lymphocyte reaction and stimulation for maturation of the differentiated DCs were 
      checked and confirmed. Importantly, this research is the first report finding 
      that the addition an extra low concentration of IL-6 and M-CSF exhibited a 
      synergistic effect with GM-CSF and IL-4 to generate higher numbers and more fully 
      functional DCs than the addition of GM-CSF and IL-4 only under serum-free 
      condition. CONCLUSION: A large number of functional DCs can be generated by using 
      SF-DC Optimal medium and provide an alternative source of DCs for related basic 
      research and clinical applications.
CI  - (c) 2023. The Author(s).
FAU - Yao, Chao-Ling
AU  - Yao CL
AD  - Department of Chemical Engineering, National Cheng Kung University, No. 1, 
      University Road, Tainan, 70101, Taiwan. yao8@gs.ncku.edu.tw.
FAU - Tseng, Tsung-Yu
AU  - Tseng TY
AD  - Department of Chemical Engineering, National Cheng Kung University, No. 1, 
      University Road, Tainan, 70101, Taiwan.
LA  - eng
GR  - MOST 108-2628-E-006-009-MY3/Ministry of Science and Technology, Taiwan/
GR  - NSTC 111-2221-E-006-013-MY3/National Science and Technology Council, Taiwan/
PT  - Journal Article
DEP - 20230126
PL  - England
TA  - J Biol Eng
JT  - Journal of biological engineering
JID - 101306640
PMC - PMC9881386
OTO - NOTNLM
OT  - Dendritic cell
OT  - IL-6
OT  - Immunotherapy
OT  - M-CSF
OT  - Serum-free
COIS- The authors declare that they have no competing interests.
EDAT- 2023/01/27 06:00
MHDA- 2023/01/27 06:01
PMCR- 2023/01/26
CRDT- 2023/01/26 23:36
PHST- 2022/11/11 00:00 [received]
PHST- 2023/01/17 00:00 [accepted]
PHST- 2023/01/26 23:36 [entrez]
PHST- 2023/01/27 06:00 [pubmed]
PHST- 2023/01/27 06:01 [medline]
PHST- 2023/01/26 00:00 [pmc-release]
AID - 10.1186/s13036-023-00325-z [pii]
AID - 325 [pii]
AID - 10.1186/s13036-023-00325-z [doi]
PST - epublish
SO  - J Biol Eng. 2023 Jan 26;17(1):6. doi: 10.1186/s13036-023-00325-z.