PMID- 36703985
OWN - NLM
STAT- MEDLINE
DCOM- 20230130
LR  - 20230202
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Perioperative corticosteroid treatment impairs tumor-infiltrating dendritic cells 
      in patients with newly diagnosed adult-type diffuse gliomas.
PG  - 1074762
LID - 10.3389/fimmu.2022.1074762 [doi]
LID - 1074762
AB  - INTRODUCTION: Adult-type diffuse gliomas are malignant primary brain tumors 
      characterized by very poor prognosis. Dendritic cells (DCs) are key in priming 
      antitumor effector functions in cancer, but their role in gliomas remains poorly 
      understood. METHODS: In this study, we characterized tumor-infiltrating DCs 
      (TIDCs) in adult patients with newly diagnosed diffuse gliomas by using 
      multi-parametric flow cytometry and single-cell RNA sequencing. RESULTS: We 
      demonstrated that different subsets of DCs are present in the glioma 
      microenvironment, whereas they are absent in cancer-free brain parenchyma. The 
      largest cluster of TIDCs was characterized by a transcriptomic profile suggestive 
      of severe functional impairment. Patients undergoing perioperative corticosteroid 
      treatment showed a significant reduction of conventional DC1s, the DC subset with 
      key functions in antitumor immunity. They also showed phenotypic and 
      transcriptional evidence of a more severe functional impairment of TIDCs. 
      DISCUSSION: Overall, the results of this study indicate that functionally 
      impaired DCs are recruited in the glioma microenvironment. They are severely 
      affected by dexamethasone administration, suggesting that the detrimental effects 
      of corticosteroids on DCs may represent one of the mechanisms contributing to the 
      already reported negative prognostic impact of steroids on glioma patient 
      survival.
CI  - Copyright (c) 2023 Carenza, Franzese, Castagna, Terzoli, Simonelli, Persico, Bello, 
      Nibali, Pessina, Kunderfranco, Peano, Balin, Mikulak, Calcaterra, Bonecchi, 
      Savino, Locati, Della Bella and Mavilio.
FAU - Carenza, Claudia
AU  - Carenza C
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
FAU - Franzese, Sara
AU  - Franzese S
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
FAU - Castagna, Alessandra
AU  - Castagna A
AD  - Laboratory of Leukocyte Biology, IRCCS Humanitas Research Hospital, Rozzano, 
      Milan, Italy.
FAU - Terzoli, Sara
AU  - Terzoli S
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
AD  - Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, 
      Italy.
FAU - Simonelli, Matteo
AU  - Simonelli M
AD  - Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, 
      Italy.
AD  - Department of Medical Oncology and Hematology, IRCCS Humanitas Research Hospital, 
      Rozzano, Milan, Italy.
FAU - Persico, Pasquale
AU  - Persico P
AD  - Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, 
      Italy.
AD  - Department of Medical Oncology and Hematology, IRCCS Humanitas Research Hospital, 
      Rozzano, Milan, Italy.
FAU - Bello, Lorenzo
AU  - Bello L
AD  - Unit of Oncological Neurosurgery, IRCCS Istituto Ortopedico Galeazzi, Milan, 
      Italy.
AD  - Department of Oncology and Hemato-Oncology, University of Milan, Milan, Italy.
FAU - Nibali, Marco Conti
AU  - Nibali MC
AD  - Unit of Oncological Neurosurgery, IRCCS Istituto Ortopedico Galeazzi, Milan, 
      Italy.
FAU - Pessina, Federico
AU  - Pessina F
AD  - Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, 
      Italy.
AD  - Department of Neurosurgery, IRCCS Humanitas Research Hospital, Rozzano, Milan, 
      Italy.
FAU - Kunderfranco, Paolo
AU  - Kunderfranco P
AD  - Bioinformatics Unit, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy.
FAU - Peano, Clelia
AU  - Peano C
AD  - Institute of Genetic and Biomedical Research, UoS Milan, National Research 
      Council, Rozzano, Milan, Italy.
FAU - Balin, Simone
AU  - Balin S
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
FAU - Mikulak, Joanna
AU  - Mikulak J
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
FAU - Calcaterra, Francesca
AU  - Calcaterra F
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
FAU - Bonecchi, Raffaella
AU  - Bonecchi R
AD  - Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, 
      Italy.
AD  - Laboratory of Chemokine Biology, IRCCS Humanitas Research Hospital, Rozzano, 
      Milan, Italy.
FAU - Savino, Benedetta
AU  - Savino B
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Leukocyte Biology, IRCCS Humanitas Research Hospital, Rozzano, 
      Milan, Italy.
FAU - Locati, Massimo
AU  - Locati M
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Leukocyte Biology, IRCCS Humanitas Research Hospital, Rozzano, 
      Milan, Italy.
FAU - Della Bella, Silvia
AU  - Della Bella S
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
FAU - Mavilio, Domenico
AU  - Mavilio D
AD  - Department of Medical Biotechnologies and Translational Medicine, University of 
      Milan, Milan, Italy.
AD  - Laboratory of Clinical and Experimental Immunology, IRCCS Humanitas Research 
      Hospital, Rozzano, Milan, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230110
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Adrenal Cortex Hormones)
SB  - IM
MH  - Humans
MH  - Adult
MH  - *Glioma
MH  - Prognosis
MH  - *Brain Neoplasms/drug therapy/pathology
MH  - Adrenal Cortex Hormones/therapeutic use
MH  - Dendritic Cells
MH  - Tumor Microenvironment
PMC - PMC9872516
OTO - NOTNLM
OT  - brain tumors
OT  - dendritic cells
OT  - immune suppressive tumor microenvironment
OT  - perioperative corticosteroids
OT  - single cell-RNA sequencing
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/28 06:00
MHDA- 2023/01/31 06:00
PMCR- 2022/01/01
CRDT- 2023/01/27 02:18
PHST- 2022/10/19 00:00 [received]
PHST- 2022/12/22 00:00 [accepted]
PHST- 2023/01/27 02:18 [entrez]
PHST- 2023/01/28 06:00 [pubmed]
PHST- 2023/01/31 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.1074762 [doi]
PST - epublish
SO  - Front Immunol. 2023 Jan 10;13:1074762. doi: 10.3389/fimmu.2022.1074762. 
      eCollection 2022.