PMID- 36704329 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230508 IS - 1662-5099 (Print) IS - 1662-5099 (Electronic) IS - 1662-5099 (Linking) VI - 15 DP - 2022 TI - Circulating exosomal microRNA profiles in migraine patients receiving acupuncture treatment: A placebo-controlled clinical trial. PG - 1098766 LID - 10.3389/fnmol.2022.1098766 [doi] LID - 1098766 AB - BACKGROUND: Acupuncture has a long history of being used in Chinese medicine for the treatment of migraine. However, molecular biomarkers for diagnosis and prognosis of migraine and its treatment are lacking. This study aimed to explore whether acupuncture could regulate differentially expressed exosomal miRNAs between patients with migraine without aura (MWoA) and healthy controls (HCs) and to identify diagnostic biomarkers that helped differentiate MWoA patients from HCs and identify prognostic biomarkers that helped to predict the effect of acupuncture. METHODS: Here, we isolated serum exosomes from patients with MWoA and HCs before and after true and sham acupuncture treatment. Then, small RNA sequencing and bioinformatics analysis were performed to screen out key miRNAs specifically responding to acupuncture treatment. Pearson's correlation analysis was used to evaluate the correlation between miRNAs and clinical phenotypes. Finally, we applied a machine learning method to identify diagnostic biomarkers of MWoA patients and identify prognostic biomarkers that helped to predict the effect of acupuncture. RESULTS: Small RNA sequencing identified 68 upregulated and 104 downregulated miRNAs in MWoA patients compared to those in HCs. Further, we identified eight upregulated and four downregulated miRNAs in migraine patients after true acupuncture treatment (trAMWoA), but not in the sham acupuncture treatment (shAMWoA) or HC group. Among them, has-miR-378a-5p was positively correlated with time unable to work, study, or do housework due to migraine (p < 0.05), whereas has-miR-605-3p was negatively correlated with the restrictive subscale of the migraine-specific quality of life questionnaire (MSQ) (p < 0.05). We then evaluated the diagnostic and prognostic potential of these 12 miRNAs in patients with MWoA. The combination of serum levels of exosomal has-miR-369-5p, has-miR-145-5p, and has-miR-5,010-3p could serve as diagnostic and prognostic biomarkers for MWoA patients following acupuncture treatment. CONCLUSION: This is the first study on the serum exosomal miRNA profiles of migraineurs before and after acupuncture treatment. Our results improve our understanding of the molecular functions of miRNAs in MWoA. More importantly, they expand our view of evaluating the clinical outcomes of migraine patients treated with acupuncture, using exosomal RNA markers. CLINICAL TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR2000034417, July 2020. CI - Copyright (c) 2023 Liu, Qi, Wang, Ni, Gao, Zhou, Chen, He, Sun, Wang, Cai and Zhao. FAU - Liu, Lu AU - Liu L AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Qi, Wenchuan AU - Qi W AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. AD - Acupuncture and Chronobiology Key Laboratory of Sichuan Province, Chengdu, Sichuan, China. FAU - Wang, Yanan AU - Wang Y AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Ni, Xixiu AU - Ni X AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Gao, Shan AU - Gao S AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Zhou, Ziyang AU - Zhou Z AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Chen, Daohong AU - Chen D AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - He, Zhenxi AU - He Z AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Sun, Mingsheng AU - Sun M AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Wang, Ziwen AU - Wang Z AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. FAU - Cai, Dingjun AU - Cai D AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. AD - Acupuncture and Chronobiology Key Laboratory of Sichuan Province, Chengdu, Sichuan, China. FAU - Zhao, Ling AU - Zhao L AD - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. AD - Acupuncture and Chronobiology Key Laboratory of Sichuan Province, Chengdu, Sichuan, China. LA - eng PT - Journal Article DEP - 20230110 PL - Switzerland TA - Front Mol Neurosci JT - Frontiers in molecular neuroscience JID - 101477914 EIN - Front Mol Neurosci. 2023 Apr 20;16:1174810. PMID: 37152427 PMC - PMC9871901 OTO - NOTNLM OT - acupuncture OT - biomarker OT - exosome OT - miRNA OT - migraine COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/01/28 06:00 MHDA- 2023/01/28 06:01 PMCR- 2022/01/01 CRDT- 2023/01/27 02:23 PHST- 2022/11/15 00:00 [received] PHST- 2022/12/19 00:00 [accepted] PHST- 2023/01/27 02:23 [entrez] PHST- 2023/01/28 06:00 [pubmed] PHST- 2023/01/28 06:01 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fnmol.2022.1098766 [doi] PST - epublish SO - Front Mol Neurosci. 2023 Jan 10;15:1098766. doi: 10.3389/fnmol.2022.1098766. eCollection 2022.