PMID- 36709365
OWN - NLM
STAT- MEDLINE
DCOM- 20230131
LR  - 20230205
IS  - 2399-3642 (Electronic)
IS  - 2399-3642 (Linking)
VI  - 6
IP  - 1
DP  - 2023 Jan 28
TI  - circRNA432 enhances the coelomocyte phagocytosis via regulating the 
      miR-2008-ELMO1 axis in Vibrio splendidus-challenged Apostichopus japonicus.
PG  - 115
LID - 10.1038/s42003-023-04516-8 [doi]
LID - 115
AB  - Circular RNAs (circRNAs) are a kind of extensive and diverse covalently closed 
      circular endogenous RNA, which exert crucial functions in immune regulation in 
      mammals. However, the functions and mechanisms of circRNAs in invertebrates are 
      largely unclarified. In our previous work, 261 differentially expressed circRNAs 
      including circRNA432 (circ432) were identified from skin ulcer syndrome (SUS) 
      diseased sea cucumber Apostichopus japonicus by RNA-seq. To better address the 
      functional role of sea cucumber circRNAs, circ432 was first found to be 
      significantly induced by Vibrio splendidus challenge and LPS exposure in this 
      study. Knock-down circ432 could depress the V. splendidus-induced coelomocytes 
      phagocytosis. Moreover, circ432 is validated to serve as the sponge of miR-2008, 
      a differential expressed miRNA in SUS-diseased sea cucumbers, by Argonaute 2-RNA 
      immunoprecipitation (AGO2-RIP) assay, luciferase reporter assay and RNA 
      fluorescence in situ hybridization (FISH) in vitro. Engulfment and cell motility 
      protein 1 (AjELMO1) is further demonstrated to be the target of miR-2008, and 
      silencing AjELMO1 inhibits the V. splendidus-induced coelomocytes phagocytosis, 
      and this phenomenon could be further suppressed by supplementing with miR-2008 
      mimics, suggesting that circ432 might regulate coelomocytes phagocytosis via 
      miR-2008-AjELMO1 axis. We further confirm that the depressed coelomocytes' 
      phagocytosis by circ432 silencing is consistent with the decreased abundance of 
      AjELMO1, and could be recovered by miR-2008 inhibitors transfection. All our 
      results provide the evidence that circ432 is involved in regulating 
      pathogen-induced coelomocyte phagocytosis via sponge miR-2008 and promotes the 
      abundance of AjELMO1. These findings will enrich the regulatory mechanism of 
      phagocytosis in echinoderm and provide theoretical data for SUS disease 
      prevention and control in sea cucumbers.
CI  - (c) 2023. The Author(s).
FAU - Fu, Xianmu
AU  - Fu X
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Ningbo University, 315211, Ningbo, P. R. China.
FAU - Guo, Ming
AU  - Guo M
AUID- ORCID: 0000-0003-4353-5331
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Ningbo University, 315211, Ningbo, P. R. China. 
      guoming@nbu.edu.cn.
FAU - Liu, Jiqing
AU  - Liu J
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Ningbo University, 315211, Ningbo, P. R. China.
FAU - Li, Chenghua
AU  - Li C
AUID- ORCID: 0000-0003-2978-8762
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Ningbo University, 315211, Ningbo, P. R. China. 
      lichenghua@nbu.edu.cn.
AD  - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology, 266071, Qingdao, P. R. 
      China. lichenghua@nbu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230128
PL  - England
TA  - Commun Biol
JT  - Communications biology
JID - 101719179
RN  - 0 (RNA, Circular)
RN  - 0 (MicroRNAs)
RN  - Vibrio splendidus
SB  - IM
MH  - Animals
MH  - *Stichopus/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Circular/genetics
MH  - Phagocytosis
MH  - *Sea Cucumbers/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Mammals/genetics
PMC - PMC9884281
COIS- The authors declare no competing interests.
EDAT- 2023/01/29 06:00
MHDA- 2023/02/01 06:00
PMCR- 2023/01/28
CRDT- 2023/01/28 23:50
PHST- 2022/08/03 00:00 [received]
PHST- 2023/01/20 00:00 [accepted]
PHST- 2023/01/29 06:00 [pubmed]
PHST- 2023/02/01 06:00 [medline]
PHST- 2023/01/28 23:50 [entrez]
PHST- 2023/01/28 00:00 [pmc-release]
AID - 10.1038/s42003-023-04516-8 [pii]
AID - 4516 [pii]
AID - 10.1038/s42003-023-04516-8 [doi]
PST - epublish
SO  - Commun Biol. 2023 Jan 28;6(1):115. doi: 10.1038/s42003-023-04516-8.