PMID- 36713031 OWN - NLM STAT- MEDLINE DCOM- 20230131 LR - 20230202 IS - 1942-0994 (Electronic) IS - 1942-0900 (Print) IS - 1942-0994 (Linking) VI - 2023 DP - 2023 TI - Electroacupuncture Alleviates 46-Trinitrobenzene Sulfonic Acid-Induced Visceral Pain via the Glutamatergic Pathway in the Prefrontal Cortex. PG - 4463063 LID - 10.1155/2023/4463063 [doi] LID - 4463063 AB - Visceral pain caused by inflammatory bowel disease (IBD) greatly diminishes the quality of life in affected patients. Yet, the mechanism of how IBD causes visceral pain is currently not fully understood. Previous studies have suggested that the central nervous system (CNS) and gut-brain axis (GBA) play an important role in IBD-inducing visceral pain. As one of the treatments for IBD, electroacupuncture (EA) has been used to treat various types of pain and gastrointestinal diseases in clinical practice. However, whether EA relieves the visceral pain of IBD through the gut-brain axis has not been confirmed. To verify the relationship between visceral pain and CNS, the following experiments were conducted. (1)H-NMR analysis was performed on the prefrontal cortex (PFC) tissue obtained from IBD rat models to determine the link between the metabolites and their role in EA treatment against visceral pain. Western blot assay was employed for detecting the contents of glutamate transporter excitatory amino acid transporters 2 (EAAT2) and the glutamate receptor N-methyl-D-aspartate (NMDA) to verify whether EA treatment can alleviate neurotoxic symptoms induced by abnormal increases of glutamate. Study results showed that the glutamate content was significantly increased in the PFC of TNBS-induced IBD rats. This change was reversed after EA treatment. This process was associated with increased EAAT2 expression and decreased expression of NMDA receptors in the PFC. In addition, an increase in intestinal glutamic-metabolizing bacteria was observed. In conclusion, this study suggests that EA treatment can relieve visceral pain by reducing glutamine toxicity in the PFC, and serves an alternative clinical utility. CI - Copyright (c) 2023 Hao Jiang et al. FAU - Jiang, Hao AU - Jiang H AUID- ORCID: 0000-0002-7590-953X AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. AD - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang310006, China. FAU - Li, Rongrong AU - Li R AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - The Third School of Clinical Medicine (School of Rehabilitation Medicine) of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. FAU - Zhang, Fan AU - Zhang F AUID- ORCID: 0000-0002-7178-8320 AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. AD - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang310006, China. FAU - Zhou, Feini AU - Zhou F AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. AD - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang310006, China. FAU - Lin, Jiangnan AU - Lin J AUID- ORCID: 0000-0003-4800-9373 AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. FAU - Kong, Ning AU - Kong N AUID- ORCID: 0000-0002-9084-0008 AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. FAU - Chen, Haitao AU - Chen H AD - The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, Zhejiang 310022, China. AD - Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. FAU - Guo, Lingnan AU - Guo L AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. AD - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang310006, China. FAU - Ye, Chenxiao AU - Ye C AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang310006, China. FAU - Li, Fuhao AU - Li F AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang310006, China. FAU - Xu, Maosheng AU - Xu M AUID- ORCID: 0000-0002-2396-1600 AD - The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. AD - Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. LA - eng PT - Journal Article DEP - 20230119 PL - United States TA - Oxid Med Cell Longev JT - Oxidative medicine and cellular longevity JID - 101479826 RN - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid) RN - 0 (Glutamates) SB - IM MH - Rats MH - Animals MH - Rats, Sprague-Dawley MH - *Visceral Pain/therapy/etiology/metabolism MH - *Electroacupuncture/methods MH - Trinitrobenzenesulfonic Acid MH - Quality of Life MH - *Inflammatory Bowel Diseases/complications MH - Prefrontal Cortex/metabolism MH - Glutamates PMC - PMC9879690 COIS- The authors declare no conflict of interest. EDAT- 2023/01/31 06:00 MHDA- 2023/02/01 06:00 PMCR- 2023/01/19 CRDT- 2023/01/30 04:09 PHST- 2022/07/05 00:00 [received] PHST- 2022/12/03 00:00 [revised] PHST- 2022/12/15 00:00 [accepted] PHST- 2023/01/30 04:09 [entrez] PHST- 2023/01/31 06:00 [pubmed] PHST- 2023/02/01 06:00 [medline] PHST- 2023/01/19 00:00 [pmc-release] AID - 10.1155/2023/4463063 [doi] PST - epublish SO - Oxid Med Cell Longev. 2023 Jan 19;2023:4463063. doi: 10.1155/2023/4463063. eCollection 2023.