PMID- 36714333
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 2452-199X (Electronic)
IS  - 2452-199X (Linking)
VI  - 24
DP  - 2023 Jun
TI  - Artificial cilia for soft and stable surface covalent immobilization of bone 
      morphogenetic protein-2.
PG  - 551-562
LID - 10.1016/j.bioactmat.2022.12.029 [doi]
AB  - Preservation of growth factor sensitivity and bioactivity (e.g., bone 
      morphogenetic protein-2 (BMP-2)) post-immobilization to tissue engineering 
      scaffolds remains a great challenge. Here, we develop a stable and soft surface 
      modification strategy to address this issue. BMP-2 (a model growth factor) is 
      covalently immobilized onto homogeneous poly (glycidyl methacrylate) (PGMA) 
      polymer brushes which are grafted onto substrate surfaces (Au, quartz glass, 
      silica wafer, or common biomaterials) via surface-initiated atom transfer radical 
      polymerization. This surface modification method multiplies the functionalized 
      interfacial area; it is simple, fast, gentle, and has little effect on the loaded 
      protein owing to the cilia motility. The immobilized BMP-2 (i-BMP-2) on the 
      surface of homogeneous PGMA polymer brushes exhibits excellent bioactivity (⁓87% 
      bioactivity of free BMP-2 in vitro and 20%-50% higher than scaffolds with free 
      BMP-2 in vivo), with conformation and secondary structure well-preserved after 
      covalent immobilization and ethanol sterilization. Moreover, the osteogenic 
      activity of i-BMP-2 on the nanoline pattern (PGMA-poly (N-isopropylacrylamide)) 
      shows ⁓110% bioactivity of free BMP-2. This is superior compared to conventional 
      protein covalent immobilization strategies in terms of both bioactivity 
      preservation and therapeutic efficacy. PGMA polymer brushes can be used to modify 
      surfaces of different tissue-engineered scaffolds, which facilitates in situ 
      immobilization of growth factors, and accelerates repair of a wide range of 
      tissue types.
CI  - (c) 2022 The Authors.
FAU - Gan, Qi
AU  - Gan Q
AD  - Laboratory for Advanced Interfacial Materials and Devices, School of Fashion and 
      Textiles, The Hong Kong Polytechnic University, Kowloon, 99077, Hong Kong Special 
      Administrative Region of China.
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, School of 
      Materials Science and Engineering, East China University of Science and 
      Technology, Shanghai, 200237, PR China.
FAU - Chen, Lina
AU  - Chen L
AD  - Laboratory for Advanced Interfacial Materials and Devices, School of Fashion and 
      Textiles, The Hong Kong Polytechnic University, Kowloon, 99077, Hong Kong Special 
      Administrative Region of China.
FAU - Bei, Ho-Pan
AU  - Bei HP
AD  - Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung 
      Hom, Hong Kong Special Administrative Region of China.
FAU - Ng, Sze-Wing
AU  - Ng SW
AD  - Laboratory for Advanced Interfacial Materials and Devices, School of Fashion and 
      Textiles, The Hong Kong Polytechnic University, Kowloon, 99077, Hong Kong Special 
      Administrative Region of China.
FAU - Guo, Han
AU  - Guo H
AD  - Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, 
      Chinese Academy of Sciences, Shanghai, 201204, PR China.
FAU - Liu, Guoqiang
AU  - Liu G
AD  - Laboratory for Advanced Interfacial Materials and Devices, School of Fashion and 
      Textiles, The Hong Kong Polytechnic University, Kowloon, 99077, Hong Kong Special 
      Administrative Region of China.
FAU - Pan, Hao
AU  - Pan H
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, School of 
      Materials Science and Engineering, East China University of Science and 
      Technology, Shanghai, 200237, PR China.
FAU - Liu, Changsheng
AU  - Liu C
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, School of 
      Materials Science and Engineering, East China University of Science and 
      Technology, Shanghai, 200237, PR China.
FAU - Zhao, Xin
AU  - Zhao X
AD  - Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung 
      Hom, Hong Kong Special Administrative Region of China.
FAU - Zheng, Zijian
AU  - Zheng Z
AD  - Laboratory for Advanced Interfacial Materials and Devices, School of Fashion and 
      Textiles, The Hong Kong Polytechnic University, Kowloon, 99077, Hong Kong Special 
      Administrative Region of China.
AD  - Department of Applied Biology and Chemical Technology, Faculty of Science, The 
      Hong Kong Polytechnic University, Kowloon, 99077, Hong Kong Special 
      Administrative Region of China.
LA  - eng
PT  - Journal Article
DEP - 20230112
PL  - China
TA  - Bioact Mater
JT  - Bioactive materials
JID - 101685294
PMC - PMC9845954
OTO - NOTNLM
OT  - Bioactivity
OT  - Poly (glycidyl methacrylate)
OT  - Polymer brush
OT  - Protein immobilization
OT  - Surface modification
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/01/31 06:00
MHDA- 2023/01/31 06:01
PMCR- 2023/01/12
CRDT- 2023/01/30 04:28
PHST- 2022/08/30 00:00 [received]
PHST- 2022/12/29 00:00 [revised]
PHST- 2022/12/29 00:00 [accepted]
PHST- 2023/01/30 04:28 [entrez]
PHST- 2023/01/31 06:00 [pubmed]
PHST- 2023/01/31 06:01 [medline]
PHST- 2023/01/12 00:00 [pmc-release]
AID - S2452-199X(22)00525-4 [pii]
AID - 10.1016/j.bioactmat.2022.12.029 [doi]
PST - epublish
SO  - Bioact Mater. 2023 Jan 12;24:551-562. doi: 10.1016/j.bioactmat.2022.12.029. 
      eCollection 2023 Jun.