PMID- 36715481
OWN - NLM
STAT- MEDLINE
DCOM- 20230131
LR  - 20230215
IS  - 1938-4351 (Electronic)
IS  - 0005-2086 (Linking)
VI  - 66
IP  - 4
DP  - 2022 Dec
TI  - Analysis of the Immune Response and Identification of Antibody Epitopes Against 
      the Sigma C Protein of Avian Orthoreovirus Following Immunization with Live or 
      Inactivated Vaccines.
PG  - 465-478
LID - 10.1637/aviandiseases-D-22-99992 [doi]
AB  - Avian orthoreoviruses are causative agents of tenosynovitis and viral arthritis 
      in both chickens and turkeys. Current commercial reovirus vaccines do not protect 
      against disease caused by emerging variants. Custom-made inactivated reovirus 
      vaccines are commonly utilized to help protect commercial poultry against 
      disease. Antibody epitopes located on the viral attachment protein, sigmaC, involved 
      in virus neutralization, have not been clearly identified. In this study, the 
      S1133 vaccine strain (Genetic Cluster 1 [GC1], a GC1 field isolate (117816), and 
      a GC5 field isolate (94826) were determined to be genetically and serologically 
      unrelated. In addition, chickens were vaccinated with either a commercial S1133 
      vaccine, 117816 GC1, or 94826 GC5, and sera were used in peptide microarrays to 
      identify linear B-cell epitopes within the sigmaC protein. Specific-pathogen-free 
      (SPF) chickens were vaccinated twice with either: 1) live and live, 2) 
      inactivated and inactivated, or 3) a combination of live and inactivated 
      vaccines. Epitope mapping was performed on individual serum samples from birds in 
      each group using S1133, 117816, and 94826 sigmaC sequences translated into an 
      overlapping peptides and spotted onto microarray chips. Vaccination with a 
      combination of live and inactivated viruses resulted in a greater number of 
      B-cell binding sites on the outer-capsid domains of sigmaC for 117816 and 94826, but 
      not for S1133. In contrast, the S1133-vaccinated birds demonstrated fewer 
      epitopes, and those epitopes were located in the stalk region of the protein. 
      However, within each of the vaccinated groups, the highest virus-neutralization 
      titers were observed in the live/inactivated groups. This study demonstrates 
      differences in antibody binding sites within sigmaC between genetically and 
      antigenically distinct reoviruses and provides initial antigenic characterization 
      of avian orthoreoviruses and insight into the inability of vaccine-induced 
      antibodies to provide adequate protection against variant reovirus-induced 
      disease.
FAU - Dawe, W H
AU  - Dawe WH
AD  - Poultry Diagnostic and Research Center, Department of Population Health, College 
      of Veterinary Medicine, University of Georgia, Athens, GA 30602.
FAU - Kapczynski, D R
AU  - Kapczynski DR
AD  - U.S. National Poultry Research Center, Agricultural Research Services, U.S. 
      Department of Agriculture, Athens, GA 30605.
FAU - Linnemann, E G
AU  - Linnemann EG
AD  - Poultry Diagnostic and Research Center, Department of Population Health, College 
      of Veterinary Medicine, University of Georgia, Athens, GA 30602.
FAU - Gauthiersloan, V R
AU  - Gauthiersloan VR
AD  - Poultry Diagnostic and Research Center, Department of Population Health, College 
      of Veterinary Medicine, University of Georgia, Athens, GA 30602.
FAU - Sellers, H S
AU  - Sellers HS
AD  - Poultry Diagnostic and Research Center, Department of Population Health, College 
      of Veterinary Medicine, University of Georgia, Athens, GA 30602, 
      hsellers@uga.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (Epitopes)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - *Orthoreovirus, Avian
MH  - Epitopes
MH  - Vaccines, Inactivated
MH  - *Poultry Diseases/prevention & control
MH  - Vaccination/veterinary
MH  - Immunity
MH  - Antibodies, Viral
OTO - NOTNLM
OT  - antibody
OT  - avian reovirus
OT  - epitope
OT  - immune response
OT  - vaccine
EDAT- 2023/01/31 06:00
MHDA- 2023/02/01 06:00
CRDT- 2023/01/30 08:41
PHST- 2022/08/15 00:00 [received]
PHST- 2022/09/01 00:00 [accepted]
PHST- 2023/01/30 08:41 [entrez]
PHST- 2023/01/31 06:00 [pubmed]
PHST- 2023/02/01 06:00 [medline]
AID - 10.1637/aviandiseases-D-22-99992 [doi]
PST - ppublish
SO  - Avian Dis. 2022 Dec;66(4):465-478. doi: 10.1637/aviandiseases-D-22-99992.