PMID- 36715911
OWN - NLM
STAT- MEDLINE
DCOM- 20230201
LR  - 20230330
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2626
DP  - 2023
TI  - Detection and Assessment of Wolbachia pipientis Infection.
PG  - 291-307
LID - 10.1007/978-1-0716-2970-3_15 [doi]
AB  - Wolbachia pipientis is a widespread vertically transmitted intracellular 
      bacterium naturally present in the model organism Drosophila melanogaster. As 
      Wolbachia is present in a large number of Drosophila lines, it is critical for 
      researchers to be able to identify which of their stocks maintain this infection 
      to avoid any potential confounding variables. Here, we describe methods for 
      detecting the bacterium and assessing the infection, including polymerase chain 
      reaction (PCR) of DNA, multi-locus sequence typing (MLST) to identify strains, 
      western blotting for protein detection, and immunohistochemistry and fluorescence 
      in situ hybridization (FISH) of Drosophila ovaries to visually detect Wolbachia 
      by fluorescence microscopy.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Nevalainen, Lindsay B M
AU  - Nevalainen LBM
AD  - Department of Biology, Indiana University, Bloomington, IN, USA.
FAU - Newton, Irene L G
AU  - Newton ILG
AD  - Department of Biology, Indiana University, Bloomington, IN, USA. 
      irnewton@indiana.edu.
LA  - eng
GR  - R01 AI144430/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - Wolbachia pipientis
SB  - IM
MH  - Animals
MH  - *Drosophila melanogaster/genetics
MH  - *Wolbachia/genetics
MH  - Multilocus Sequence Typing
MH  - In Situ Hybridization, Fluorescence
MH  - Drosophila/genetics
PMC - PMC10046185
MID - NIHMS1880372
OTO - NOTNLM
OT  - Drosophila
OT  - Endosymbiont
OT  - FISH
OT  - Fluorescence microscopy
OT  - Immunolabeling
OT  - MLST
OT  - Microbiome
OT  - PCR
OT  - Western blotting
OT  - Wolbachia
EDAT- 2023/01/31 06:00
MHDA- 2023/02/02 06:00
PMCR- 2023/03/28
CRDT- 2023/01/30 11:27
PHST- 2023/01/30 11:27 [entrez]
PHST- 2023/01/31 06:00 [pubmed]
PHST- 2023/02/02 06:00 [medline]
PHST- 2023/03/28 00:00 [pmc-release]
AID - 10.1007/978-1-0716-2970-3_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2626:291-307. doi: 10.1007/978-1-0716-2970-3_15.