PMID- 36716712
OWN - NLM
STAT- MEDLINE
DCOM- 20230705
LR  - 20230705
IS  - 1423-0194 (Electronic)
IS  - 0028-3835 (Linking)
VI  - 113
IP  - 7
DP  - 2023
TI  - Ultrastructural and Molecular Evidence of Macroautophagy in Functioning PitNETs 
      and Experimental Pituitary Tumors.
PG  - 705-718
LID - 10.1159/000529416 [doi]
AB  - INTRODUCTION: Macroautophagy is a lysosome-mediated degradation process that 
      controls the quality of cytoplasmic components and organelles, with its 
      regulation depending on autophagy-related proteins (Atg) and with Beclin1/Atg6 
      and microtubule-associated protein light chain 3 (LC3/Atg8) being key players in 
      the mammalian autophagy. As reports on this mechanism in the field of pituitary 
      neuropathology and neuroendocrinology are scarce, our study analyzed the 
      ultrastructural signs of macroautophagy and the expression of Beclin1 and LC3 
      proteins in human functioning PitNETs and in experimental pituitary tumors. 
      METHODS: A group of humans functioning PitNETs and an experimental lactotroph 
      model in rats of the F344 strain stimulated with estradiol benzoate (BE) were 
      used. Ultrastructural and molecular evidence of the macroautophagic process was 
      evaluated using different techniques. RESULTS: In functioning PitNETs cohort, 60% 
      exhibited evidence of macroautophagy, with a significant difference found for 
      Beclin1 and LC3 between macro- and micro-PitNETs (p < 0.05). In the 
      experimental model, the expression of both Beclin1 and LC3 proteins was 
      immunopositive in normal and tumoral glands when analyzed by immunofluorescence, 
      Western blot, and immunohistochemistry. In the experimental model, protein 
      expression was associated with increased glandular size and weight. CONCLUSIONS: 
      Our study revealed evidence of macroautophagy at the pituitary level and the 
      important role of Beclin1 and LC3 in the progression of functioning PitNETs, 
      implying that this mechanism participate in regulating pituitary cell growth.
CI  - (c) 2023 S. Karger AG, Basel.
FAU - Cecenarro, Laura Anahi
AU  - Cecenarro LA
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Hospital Privado Universitario de Cordoba, Cordoba, Argentina.
FAU - Moyano Crespo, Gabriela Deisi
AU  - Moyano Crespo GD
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
FAU - Guido, Carolina Beatriz
AU  - Guido CB
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
FAU - Perez, Pablo Anibal
AU  - Perez PA
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Cordoba, Argentina.
FAU - Faure, Erica Elizabeth
AU  - Faure EE
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
FAU - Picech, Florencia
AU  - Picech F
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
FAU - Rossetto, Sofia Victoria
AU  - Rossetto SV
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Cordoba, Argentina.
FAU - De Battista, Juan Carlos
AU  - De Battista JC
AD  - Hospital Privado Universitario de Cordoba, Cordoba, Argentina.
FAU - Gutierrez, Silvina
AU  - Gutierrez S
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Cordoba, Argentina.
FAU - Torres, Alicia Ines
AU  - Torres AI
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Cordoba, Argentina.
FAU - Petiti, Juan Pablo
AU  - Petiti JP
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Cordoba, Argentina.
FAU - Mukdsi, Jorge Humberto
AU  - Mukdsi JH
AD  - Centro de Microscopia Electronica, Facultad de Ciencias Medicas, Universidad 
      Nacional de Cordoba, Cordoba, Argentina.
AD  - Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Cordoba, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230130
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Beclin-1)
RN  - 0 (Microtubule-Associated Proteins)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - Beclin-1
MH  - *Macroautophagy
MH  - *Pituitary Neoplasms
MH  - Rats, Inbred F344
MH  - Autophagy
MH  - Microtubule-Associated Proteins/metabolism
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Beclin1
OT  - Electronmicroscopy
OT  - LC3II
OT  - Macroautophagy
OT  - Pituitary tumor
EDAT- 2023/01/31 06:00
MHDA- 2023/07/05 06:42
CRDT- 2023/01/30 18:22
PHST- 2022/04/26 00:00 [received]
PHST- 2023/01/23 00:00 [accepted]
PHST- 2023/07/05 06:42 [medline]
PHST- 2023/01/31 06:00 [pubmed]
PHST- 2023/01/30 18:22 [entrez]
AID - 000529416 [pii]
AID - 10.1159/000529416 [doi]
PST - ppublish
SO  - Neuroendocrinology. 2023;113(7):705-718. doi: 10.1159/000529416. Epub 2023 Jan 
      30.