PMID- 36718148
OWN - NLM
STAT- MEDLINE
DCOM- 20230201
LR  - 20230202
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2023
DP  - 2023
TI  - Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic 
      Biomarker for Laryngeal Squamous Cell Cancer.
PG  - 1733100
LID - 10.1155/2023/1733100 [doi]
LID - 1733100
AB  - Laryngeal squamous cell cancer (LSCC) is a common malignant tumor with a high 
      degree of malignancy, and its etiology remains unclear. Therefore, screening 
      potential biomarkers is necessary to facilitate the treatment and diagnosis of 
      LSCC. Robust rank aggregation (RRA) analysis was used to integrate two gene 
      expression datasets of LSCC patients from the Gene Expression Omnibus (GEO) 
      database and identify differentially expressed genes (DEGs) between LSCC and 
      nonneoplastic tissues. A gene coexpression network was constructed using weighted 
      gene correlation network analysis (WGCNA) to explore potential associations 
      between the module genes and clinical features of LSCC. Combining differential 
      gene expression analysis and survival analysis, we screened potential hub genes, 
      including CDK1, SPC24, HOXB7, and SELENBP1. Subsequently, western blotting and 
      immunohistochemistry were used to test the protein levels in clinical specimens 
      to verify our findings. Finally, four candidate diagnostic and prognostic 
      biomarkers (CDK1, SPC24, HOXB7, and SELENBP1) were identified. We propose, for 
      the first time, that SPC24 is a gene that may associate with LSCC malignancy and 
      is a novel therapeutic target. These findings may provide important mechanistic 
      insight of LSCC.
CI  - Copyright (c) 2023 Jialei Chen et al.
FAU - Chen, Jialei
AU  - Chen J
AUID- ORCID: 0000-0002-6834-4219
AD  - Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical 
      University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
AD  - Department of Pathology and Pathophysiology, Chongqing Medical University, 
      Chongqing, China.
AD  - Molecular Medicine and Cancer Research Center, College of Basic Medical Sciences, 
      Chongqing Medical University, Chongqing 400016, China.
FAU - Luo, Jing
AU  - Luo J
AUID- ORCID: 0000-0002-9778-1012
AD  - Molecular Medicine and Cancer Research Center, College of Basic Medical Sciences, 
      Chongqing Medical University, Chongqing 400016, China.
AD  - Department of Pathology, Chongqing Medical University, Chongqing, China.
AD  - Department of Pathology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - He, Jing
AU  - He J
AUID- ORCID: 0000-0003-3339-482X
AD  - Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical 
      University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
FAU - Jiang, Xianyao
AU  - Jiang X
AUID- ORCID: 0000-0003-3192-1235
AD  - Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical 
      University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
FAU - Jiang, Ning
AU  - Jiang N
AUID- ORCID: 0000-0002-5784-8622
AD  - Department of Pathology, Chongqing Medical University, Chongqing, China.
AD  - Department of Pathology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Yang, Changhong
AU  - Yang C
AUID- ORCID: 0000-0002-7573-3765
AD  - Department of Bioinformatics, College of Basic Medicine, Chongqing Medical 
      University, Chongqing 400016, China.
FAU - Zhong, Shixun
AU  - Zhong S
AUID- ORCID: 0000-0001-5643-1000
AD  - Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical 
      University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
LA  - eng
PT  - Journal Article
DEP - 20230121
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (SPC24 protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (HOXB7 protein, human)
RN  - 0 (Homeodomain Proteins)
SB  - IM
MH  - Humans
MH  - *Laryngeal Neoplasms/diagnosis/genetics/metabolism
MH  - Prognosis
MH  - Squamous Cell Carcinoma of Head and Neck/diagnosis/genetics
MH  - *Head and Neck Neoplasms/genetics
MH  - Cell Cycle/genetics
MH  - *Neoplasms, Squamous Cell
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Microtubule-Associated Proteins/genetics
MH  - Homeodomain Proteins/genetics
PMC - PMC9884166
COIS- It should be noted that none of the authors have any commercial or financial 
      relationships that might be construed as a conflict of interest.
EDAT- 2023/02/01 06:00
MHDA- 2023/02/02 06:00
PMCR- 2023/01/21
CRDT- 2023/01/31 01:43
PHST- 2022/02/23 00:00 [received]
PHST- 2022/10/02 00:00 [revised]
PHST- 2022/12/27 00:00 [accepted]
PHST- 2023/01/31 01:43 [entrez]
PHST- 2023/02/01 06:00 [pubmed]
PHST- 2023/02/02 06:00 [medline]
PHST- 2023/01/21 00:00 [pmc-release]
AID - 10.1155/2023/1733100 [doi]
PST - epublish
SO  - Biomed Res Int. 2023 Jan 21;2023:1733100. doi: 10.1155/2023/1733100. eCollection 
      2023.