PMID- 36719505
OWN - NLM
STAT- MEDLINE
DCOM- 20230508
LR  - 20230508
IS  - 1436-3771 (Electronic)
IS  - 1432-6981 (Linking)
VI  - 27
IP  - 5
DP  - 2023 May
TI  - Toll-like receptor-4 activation by subgingival biofilm and periodontal treatment 
      response.
PG  - 2139-2147
LID - 10.1007/s00784-023-04877-8 [doi]
AB  - OBJECTIVES: This study aims to investigate longitudinally the activation of 
      Toll-like receptor-4 (TLR-4) by subgingival biofilm samples before and after 
      nonsurgical periodontal therapy (NSPT). MATERIALS AND METHODS: Forty 
      periodontitis patients received NSPT and were reviewed 3 and 6 months 
      post-treatment. Subgingival biofilm was sampled from 4 teeth per patient, at 
      baseline and each follow-up time point. TLR-4 activation was determined using the 
      HEK-BLUE/hTLR4 system. Changes in TLR-4 activation and probing pocket depths 
      (PPDs) were evaluated using generalised linear models, and the association 
      between TLR-4 activation and pocket reduction (defined as 6-month PPDs </= 3mm) was 
      determined using generalised estimating equations. RESULTS: At 6 months, the mean 
      TLR-4 activation by subgingival biofilm samples was significantly reduced from 
      11.2AU (95%CI 7.1AU, 15.4AU) to 3.6AU (95%CI 2.3AU, 4.8AU, p < 0.001), 
      paralleling significant reductions in mean PPDs at sampled sites. The response to 
      NSPT was associated with longitudinal TLR-4 activation profiles, with 
      significantly higher TLR-4 activation by subgingival biofilm obtained from sites 
      that did not achieve pocket reduction, compared to sites at which pocket 
      reduction was achieved. CONCLUSIONS: The activation of TLR-4 by subgingival 
      biofilm samples was reduced after NSPT, and this reduction was significantly 
      associated with the clinical improvements (PPD reductions) at sampled sites. 
      CLINICAL RELEVANCE: This study demonstrated an association between the 
      longitudinal profile of TLR-4 activation by subgingival biofilm and periodontal 
      treatment response. Longitudinal monitoring of TLR-4 activation by subgingival 
      biofilm may potentially identify non-responsive sites, enabling targeted 
      additional treatment.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Chew, Ren Jie Jacob
AU  - Chew RJJ
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore.
FAU - Tang, Yi Ling
AU  - Tang YL
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore.
FAU - Lin, Xin Yi Sheena
AU  - Lin XYS
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore.
FAU - Oh, Feng Jun Bryan
AU  - Oh FJB
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore.
FAU - Sim, Ruiqi Paul
AU  - Sim RP
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore.
FAU - Anwar, Erica Jade
AU  - Anwar EJ
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore.
FAU - Preshaw, Philip M
AU  - Preshaw PM
AD  - School of Dentistry, University of Dundee, Park Place, Dundee, DD1 4HN, UK. 
      ppreshaw001@dundee.ac.uk.
FAU - Tan, Kai Soo
AU  - Tan KS
AD  - Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, 
      Singapore, 119085, Singapore. denkst@nus.edu.sg.
AD  - Oral Care Health Innovations and Designs Singapore, National University of 
      Singapore, 9 Lower Kent Ridge Road, Singapore, 119085, Singapore. 
      denkst@nus.edu.sg.
LA  - eng
PT  - Journal Article
DEP - 20230131
PL  - Germany
TA  - Clin Oral Investig
JT  - Clinical oral investigations
JID - 9707115
RN  - 0 (Toll-Like Receptor 4)
MH  - Humans
MH  - Periodontal Pocket/therapy
MH  - *Toll-Like Receptor 4
MH  - *Periodontitis/drug therapy
OTO - NOTNLM
OT  - Biomarkers
OT  - Endotoxins
OT  - Lipopolysaccharides
OT  - Periodontal debridement
OT  - Periodontitis
EDAT- 2023/02/01 06:00
MHDA- 2023/05/08 06:41
CRDT- 2023/01/31 11:16
PHST- 2022/09/06 00:00 [received]
PHST- 2023/01/22 00:00 [accepted]
PHST- 2023/05/08 06:41 [medline]
PHST- 2023/02/01 06:00 [pubmed]
PHST- 2023/01/31 11:16 [entrez]
AID - 10.1007/s00784-023-04877-8 [pii]
AID - 10.1007/s00784-023-04877-8 [doi]
PST - ppublish
SO  - Clin Oral Investig. 2023 May;27(5):2139-2147. doi: 10.1007/s00784-023-04877-8. 
      Epub 2023 Jan 31.