PMID- 36721751 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230202 IS - 2287-1098 (Print) IS - 2287-8602 (Electronic) IS - 2287-1098 (Linking) VI - 27 IP - 4 DP - 2022 Dec 31 TI - Pluchea indica Leaf Extract Alleviates Dyslipidemia and Hepatic Steatosis by Modifying the Expression of Lipid Metabolism-Related Genes in Rats Fed a High Fat-High Fructose Diet. PG - 384-398 LID - 10.3746/pnf.2022.27.4.384 [doi] AB - This study evaluated the effect of Pluchea indica leaf extract (PIE) on dyslipidemia and lipid accumulation in the liver, emphasizing its molecular mechanisms in regulating lipid metabolism in rats fed a high fat-high fructose diet (HFFD). Male rats were fed HFFD (40% lard and 20% fructose) for ten weeks. They were then divided into four groups receiving distilled water, PIE (100 or 300 mg/kg/d), and pioglitazone (10 mg/kg/d) for a further six weeks, during which the HFFD was continued. After the experiment, fasting blood glucose (FBG), oral glucose tolerance (OGT), serum insulin and leptin levels, lipid profiles, and hepatic triglyceride content were measured. Histological examination and expression levels of lipid metabolism-related genes in the liver were measured. HFFD-fed rats indicated a significantly increased FBG, serum leptin, and homeostasis model assessment of insulin resistance (HOMA-IR) scores with impaired OGT and dyslipidemia compared to rats fed a normal diet. PIE significantly reduced FBG, serum leptin, and HOMA-IR scores and improved OGT. Additionally, PIE significantly improved dyslipidemia and decreased serum-free fatty acids and liver triglyceride content. Hepatic histological examination showed a marked reduction lipid accumulation in relation to HFFD controls. Interestingly, PIE significantly downregulated the expression of lipid synthesis-related genes and upregulated the expression of fatty-acid oxidation-related genes. In conclusion, PIE alleviates dyslipidemia and hepatic steatosis in HFFD rats plausibly by increasing insulin resistance and modifying the gene expression associated with lipid metabolism. PIE may be used as preventive nutrition for dyslipidemia and hepatic steatosis. CI - Copyright (c) 2022 by The Korean Society of Food Science and Nutrition. All rights Reserved. FAU - Singdam, Patcharin AU - Singdam P AUID- ORCID: 0000-0001-5847-950X AD - Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Naowaboot, Jarinyaporn AU - Naowaboot J AUID- ORCID: 0000-0003-0140-8801 AD - Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand. FAU - Senggunprai, Laddawan AU - Senggunprai L AUID- ORCID: 0000-0001-5509-8664 AD - Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Boonloh, Kampeebhorn AU - Boonloh K AUID- ORCID: 0000-0002-7051-8417 AD - Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Pannangpetch, Patchareewan AU - Pannangpetch P AUID- ORCID: 0000-0003-0183-3592 AD - Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. LA - eng PT - Journal Article PL - Korea (South) TA - Prev Nutr Food Sci JT - Preventive nutrition and food science JID - 101586663 PMC - PMC9843721 OTO - NOTNLM OT - Pluchea indica (L.) OT - dyslipidemias OT - hepatic steatosis OT - non-alcoholic fatty liver disease COIS- AUTHOR DISCLOSURE STATEMENT The authors declare no conflicts of interest. EDAT- 2023/02/02 06:00 MHDA- 2023/02/02 06:01 PMCR- 2022/12/31 CRDT- 2023/02/01 01:50 PHST- 2022/05/26 00:00 [received] PHST- 2022/08/23 00:00 [revised] PHST- 2022/09/26 00:00 [accepted] PHST- 2023/02/01 01:50 [entrez] PHST- 2023/02/02 06:00 [pubmed] PHST- 2023/02/02 06:01 [medline] PHST- 2022/12/31 00:00 [pmc-release] AID - pnfs-27-4-384 [pii] AID - 10.3746/pnf.2022.27.4.384 [doi] PST - ppublish SO - Prev Nutr Food Sci. 2022 Dec 31;27(4):384-398. doi: 10.3746/pnf.2022.27.4.384.