PMID- 36725266
OWN - NLM
STAT- MEDLINE
DCOM- 20230203
LR  - 20230203
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 1
DP  - 2023 Jan
TI  - [Hepatotoxicity and mechanism of Rhododendri Mollis Flos based on zebrafish 
      model].
PG  - 140-147
LID - 10.19540/j.cnki.cjcmm.20220914.401 [doi]
AB  - This study used the zebrafish model to explore the hepatotoxicity of Rhododendri 
      Mollis Flos(RMF). The mortality was calculated according to the number of the 
      survival of zebrafish larvae 4 days after fertilization under different 
      concentration of RMF, and the dose-toxicity curve was fitted to preliminarily 
      evaluate the toxicity of RMF. The liver phenotypes under the sublethal 
      concentration of RMF in the treatment group and the blank control group were 
      observed by hematoxylin-eosin(HE) staining and acridine orange(AO) staining. 
      Meanwhile, the activities of alanine aminotransferase(ALT) and aspartate 
      aminotransferase(AST) were determined to confirm the hepatotoxicity of RMF. 
      Real-time quantitative polymerase chain reaction(real-time PCR) and Western blot 
      were used to determine the expressions of genes and proteins in zebrafish larvae. 
      Gas chromatography time-of-flight mass spectrometry(GC-TOF-MS) was used to 
      conduct untargeted metabolomics testing to explore the mechanism. The results 
      showed that the toxicity of RMF to zebrafish larvae was dose-dependent, with 1 
      100 mug.mL~(-1) of the absolute lethal concentration and 448 mug.mL~(-1) of 
      sublethal concentration. The hepatocyte apoptosis and degeneration appeared in 
      the zebrafish larvae under the sublethal concentration of RMF. The content of ALT 
      and AST in zebrafish larvae at the end of the experiment was significantly 
      increased in a dose-dependent manner. Under the sublethal concentration, the 
      expressions of genes and proteins related to apoptosis in zebrafish larvae were 
      significantly increased as compared with the blank control group. The results of 
      untargeted metabolomics showed that the important metabolites related to the 
      he-patotoxicity of RMF were mainly enriched in alanine, aspartic acid, glutamic 
      acid, and other pathways. In conclusion, it is inferred that RMF has certain 
      hepatotoxicity to zebrafish larvae, and its mechanism may be related to 
      apoptosis.
FAU - Chen, Mei-Lin
AU  - Chen ML
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Li, Zhi-Qi
AU  - Li ZQ
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Fan, Qi-Qi
AU  - Fan QQ
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Guo, Si-Min
AU  - Guo SM
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Cai, Qiong
AU  - Cai Q
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Lin, Rui-Chao
AU  - Lin RC
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Wu, Jia-Rui
AU  - Wu JR
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
FAU - Zhao, Chong-Jun
AU  - Zhao CJ
AD  - Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School 
      of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
SB  - IM
MH  - Animals
MH  - *Zebrafish/genetics
MH  - Apoptosis
MH  - Larva
MH  - *Chemical and Drug Induced Liver Injury
OTO - NOTNLM
OT  - Rhododendri Mollis Flos
OT  - apoptosis
OT  - hepatotoxicity
OT  - zebrafish
EDAT- 2023/02/02 06:00
MHDA- 2023/02/04 06:00
CRDT- 2023/02/01 21:03
PHST- 2023/02/01 21:03 [entrez]
PHST- 2023/02/02 06:00 [pubmed]
PHST- 2023/02/04 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20220914.401 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Jan;48(1):140-147. doi: 
      10.19540/j.cnki.cjcmm.20220914.401.