PMID- 36729014
OWN - NLM
STAT- MEDLINE
DCOM- 20230606
LR  - 20230614
IS  - 1097-0134 (Electronic)
IS  - 0887-3585 (Linking)
VI  - 91
IP  - 7
DP  - 2023 Jul
TI  - Investigation of biological activities of two cultivars of Cicer arietinum 
      proteins mass associated with Alzheimer's disease.
PG  - 859-871
LID - 10.1002/prot.26472 [doi]
AB  - Alzheimer's disease (AD) is the most common cause of dementia in the elderly, 
      with some known classical factors. Cicer arietinum (Leguminosae) is a source of 
      protein for humans and contains albumin, globulin, glutelin, and prolamin. The 
      protein content of two cultivars of C. arietinum, Hashem and Mansour, was 
      isolated to evaluate their inhibition activity against acetylcholinesterase 
      (AChE), butyrylcholine esterase (BChE), and beta-amyloid peptide (betaA) aggregation. 
      Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 
      molecular docking were also applied to evaluate the content and determine the 
      potential of each chickpea protein to interact with AChE, respectively. Obtained 
      data showed that proteins from both cultivars could inhibit AChE with IC50 of 
      17.73 (0.03) and 22.20 (0.06) mug/mL, respectively, with no activity on BChE. The 
      50 mug/mL protein concentration of each cultivar suppressed betaA accumulation 
      (Mansour: 25.66% and Hashem: 21.69%) and showed biometal chelating activity. 
      SDS-PAGE analysis revealed relatively different protein patterns, though the 
      Mansour cultivar contained some protein bands with molecular weights of 18, 24, 
      and 70 kDa were estimated to belong to vicilin and legumin, which were absent in 
      the Hashem protein mass. Molecular docking showed that legumin and especially 
      vicilin have good potential to interact with AChE. The chickpea proteins showed 
      inhibitory activity against AChE, which might be due to the vicilin and legumin 
      fractions. The characterization of the inhibitory effect of each protein band 
      could be promising in finding new therapeutic peptide candidates to treat 
      Alzheimer's in the future, although more experimental work is needed in this 
      issue.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Mafakher, Ladan
AU  - Mafakher L
AD  - Thalassemia & Hemoglobinopathy Research center, Health research institute, Ahvaz 
      Jundishapur University of Medical Sciences, Ahvaz, Iran.
FAU - Rismani, Elham
AU  - Rismani E
AD  - Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute 
      of Iran, Tehran, Iran.
FAU - Saeedi, Mina
AU  - Saeedi M
AD  - Persian Medicine and Pharmacy Research Center, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Emami, Mir Mohammad Reza Hosseini Moghadam
AU  - Emami MMRHM
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Hadjiakhoondi, Abbas
AU  - Hadjiakhoondi A
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of 
      Medical Sciences, Tehran, Iran.
FAU - Najafi, Zahra
AU  - Najafi Z
AD  - Department of Medicinal Chemistry, School of Pharmacy, Hamedan University of 
      Medical Sciences, Hamedan, Iran.
FAU - Manayi, Azadeh
AU  - Manayi A
AUID- ORCID: 0000-0002-0420-5930
AD  - Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of 
      Medical Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230221
PL  - United States
TA  - Proteins
JT  - Proteins
JID - 8700181
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Cholinesterase Inhibitors)
SB  - IM
MH  - Humans
MH  - Aged
MH  - *Alzheimer Disease/drug therapy/metabolism
MH  - *Cicer/chemistry/metabolism
MH  - Acetylcholinesterase/metabolism
MH  - Molecular Docking Simulation
MH  - Amyloid beta-Peptides
MH  - Cholinesterase Inhibitors/pharmacology
OTO - NOTNLM
OT  - SDS-PAGE
OT  - acetylcholinesterase
OT  - butyrylcholine esterase
OT  - chickpea
OT  - molecular docking
OT  - beta-Amyloid peptide
EDAT- 2023/02/03 06:00
MHDA- 2023/06/06 06:42
CRDT- 2023/02/02 10:46
PHST- 2023/01/11 00:00 [revised]
PHST- 2022/08/16 00:00 [received]
PHST- 2023/01/30 00:00 [accepted]
PHST- 2023/06/06 06:42 [medline]
PHST- 2023/02/03 06:00 [pubmed]
PHST- 2023/02/02 10:46 [entrez]
AID - 10.1002/prot.26472 [doi]
PST - ppublish
SO  - Proteins. 2023 Jul;91(7):859-871. doi: 10.1002/prot.26472. Epub 2023 Feb 21.