PMID- 36731877
OWN - NLM
STAT- MEDLINE
DCOM- 20230309
LR  - 20230309
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 76
IP  - 3
DP  - 2023 Mar 1
TI  - Maturation of pathogenic biofilms induces enhanced resistance against gaseous 
      chlorine dioxide.
LID - ovad016 [pii]
LID - 10.1093/lambio/ovad016 [doi]
AB  - Biofilms are surface-associated microbial clusters embedded in extracellular 
      polymeric substances. Biofilms formed on food-contact surfaces create challenges 
      for the food industry due to their increased tolerance to antimicrobial agents 
      and disinfectants. This study aimed to evaluate the effect of the biofilm 
      maturation period on their resistance to gaseous ClO2. Listeria monocytogenes, 
      Salmonellaserotype Typhimurium, and Escherichia coli O157:H7 biofilms formed on 
      stainless steel (SS) and high-density polyethylene (HDPE) surfaces were 
      investigated. The total cell mass and protein content significantly increased 
      (P < .05) between the second and the fifth day of maturation, and the biofilms' 
      resistance to gaseous ClO2 increased as they matured. Generally, the cell counts 
      of 0-day-old L. monocytogenes, Salm. Typhimurium, and E. coli O157:H7 biofilms on 
      SS and HDPE reduced below the detection limit (0.48 log CFU/cm2) within 5 min. 
      The cell counts of 2-day-old biofilms of the three pathogens were reduced by 6.22 
      to over 7.52 log, while those of 5-day-old biofilms were reduced by 3.64 to over 
      6.34 log after 20 min of treatment with 30 ppmv of gaseous ClO2. Therefore, as 
      resistance increases with biofilm maturation, daily gaseous ClO2 treatment would 
      maximize the antimicrobial efficacy of the cleaning strategy against biofilms.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of Applied 
      Microbiology International.
FAU - Kim, Se-Yeon
AU  - Kim SY
AD  - Department of Food Science and Technology, Kongju National University, Yesan, 
      Chungnam 32439, Republic of Korea.
FAU - Park, SangHyun
AU  - Park S
AUID- ORCID: 0000-0003-2119-7845
AD  - Department of Food Science and Technology, Kongju National University, Yesan, 
      Chungnam 32439, Republic of Korea.
LA  - eng
GR  - NRF-2020R1F1A1048617/National Research Foundation of Korea/
GR  - 2021332C10-2123A01/Korea Forest Service/
PT  - Journal Article
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 8061YMS4RM (chlorine dioxide)
RN  - 0 (Gases)
RN  - 9002-88-4 (Polyethylene)
RN  - 0 (Anti-Infective Agents)
SB  - IM
MH  - Gases/pharmacology
MH  - Colony Count, Microbial
MH  - Polyethylene/pharmacology
MH  - Food Microbiology
MH  - Biofilms
MH  - *Escherichia coli O157
MH  - *Anti-Infective Agents/pharmacology
OTO - NOTNLM
OT  - Listeria
OT  - Salmonella
OT  - biofilms
OT  - non-thermal processes
OT  - pathogenic Escherichia coli
OT  - sanitation
EDAT- 2023/02/03 06:00
MHDA- 2023/03/10 06:00
CRDT- 2023/02/02 20:03
PHST- 2022/08/29 00:00 [received]
PHST- 2022/12/26 00:00 [revised]
PHST- 2023/02/01 00:00 [accepted]
PHST- 2023/02/03 06:00 [pubmed]
PHST- 2023/03/10 06:00 [medline]
PHST- 2023/02/02 20:03 [entrez]
AID - 7024855 [pii]
AID - 10.1093/lambio/ovad016 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2023 Mar 1;76(3):ovad016. doi: 10.1093/lambio/ovad016.