PMID- 36732743 OWN - NLM STAT- MEDLINE DCOM- 20230206 LR - 20230215 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 21 IP - 1 DP - 2023 Feb 2 TI - Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues. PG - 68 LID - 10.1186/s12967-023-03938-6 [doi] LID - 68 AB - BACKGROUND: Intratumoral microbial communities have been recently discovered to exist in a variety of cancers and have been found to be intricately involved in tumour progression. Therefore, investigating the profiles and functions of intratumoral microbial distribution in hepatocellular carcinoma (HCC) is imperative. METHODS: To verify the presence of microorganisms in HCC, we performed fluorescence in situ hybridization (FISH) using HCC tissues and conducted MiSeq using 99 HCC and paracancerous tissues to identify the key microorganisms and changes in metabolic pathways affecting HCC progression through a variety of bioinformatics methods. RESULTS: Microbial diversity was significantly higher in HCC tissues than in adjacent tissues. The abundances of microorganisms such as Enterobacteriaceae, Fusobacterium and Neisseria were significantly increased in HCC tissues, while the abundances of certain antitumour bacteria such as Pseudomonas were decreased. Processes such as fatty acid and lipid synthesis were significantly enhanced in the microbiota in HCC tissues, which may be a key factor through which intratumoral microbes influence tumour progression. There were considerable differences in the microbes and their functions within tumour tissue collected from patients with different clinical features. CONCLUSION: We comprehensively evaluated the intratumoral microbial atlas of HCC tissue and preliminarily explored the mechanism of the effects of the microbial community involving changes in lipid metabolism and effects on HCC progression, which lays the foundation for further research in this field. CI - (c) 2023. The Author(s). FAU - He, Yuting AU - He Y AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. fccheyt1@zzu.edu.cn. AD - Key Laboratory of Hepatobiliary and Pancreatic Surgery and Digestive Organ Transplantation of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. fccheyt1@zzu.edu.cn. AD - Open and Key Laboratory of Hepatobiliary & Pancreatic Surgery and Digestive Organ Transplantation at Henan Universities, Zhengzhou, 450052, China. fccheyt1@zzu.edu.cn. AD - Henan Key Laboratory of Digestive Organ Transplantation, Zhengzhou, 450052, China. fccheyt1@zzu.edu.cn. FAU - Zhang, Qiyao AU - Zhang Q AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AD - Key Laboratory of Hepatobiliary and Pancreatic Surgery and Digestive Organ Transplantation of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AD - Open and Key Laboratory of Hepatobiliary & Pancreatic Surgery and Digestive Organ Transplantation at Henan Universities, Zhengzhou, 450052, China. AD - Henan Key Laboratory of Digestive Organ Transplantation, Zhengzhou, 450052, China. FAU - Yu, Xiao AU - Yu X AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AD - Key Laboratory of Hepatobiliary and Pancreatic Surgery and Digestive Organ Transplantation of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AD - Open and Key Laboratory of Hepatobiliary & Pancreatic Surgery and Digestive Organ Transplantation at Henan Universities, Zhengzhou, 450052, China. AD - Henan Key Laboratory of Digestive Organ Transplantation, Zhengzhou, 450052, China. FAU - Zhang, Shuijun AU - Zhang S AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AD - Key Laboratory of Hepatobiliary and Pancreatic Surgery and Digestive Organ Transplantation of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AD - Open and Key Laboratory of Hepatobiliary & Pancreatic Surgery and Digestive Organ Transplantation at Henan Universities, Zhengzhou, 450052, China. AD - Henan Key Laboratory of Digestive Organ Transplantation, Zhengzhou, 450052, China. FAU - Guo, Wenzhi AU - Guo W AUID- ORCID: 0000-0002-0821-1318 AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. fccguowz@zzu.edu.cn. AD - Key Laboratory of Hepatobiliary and Pancreatic Surgery and Digestive Organ Transplantation of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. fccguowz@zzu.edu.cn. AD - Open and Key Laboratory of Hepatobiliary & Pancreatic Surgery and Digestive Organ Transplantation at Henan Universities, Zhengzhou, 450052, China. fccguowz@zzu.edu.cn. AD - Henan Key Laboratory of Digestive Organ Transplantation, Zhengzhou, 450052, China. fccguowz@zzu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230202 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 SB - IM MH - Humans MH - *Carcinoma, Hepatocellular/genetics/pathology MH - *Liver Neoplasms/genetics/pathology MH - In Situ Hybridization, Fluorescence MH - Computational Biology PMC - PMC9893660 OTO - NOTNLM OT - Fatty acid and lipid synthesis OT - Hepatocellular carcinoma OT - Intratumoral microbiota OT - Metabolic pathway OT - MiSeq COIS- The authors declare that they have no competing interests. EDAT- 2023/02/03 06:00 MHDA- 2023/02/07 06:00 PMCR- 2023/02/02 CRDT- 2023/02/02 23:59 PHST- 2022/07/21 00:00 [received] PHST- 2023/01/29 00:00 [accepted] PHST- 2023/02/02 23:59 [entrez] PHST- 2023/02/03 06:00 [pubmed] PHST- 2023/02/07 06:00 [medline] PHST- 2023/02/02 00:00 [pmc-release] AID - 10.1186/s12967-023-03938-6 [pii] AID - 3938 [pii] AID - 10.1186/s12967-023-03938-6 [doi] PST - epublish SO - J Transl Med. 2023 Feb 2;21(1):68. doi: 10.1186/s12967-023-03938-6.