PMID- 3673518
OWN - NLM
STAT- MEDLINE
DCOM- 19871209
LR  - 20200304
IS  - 0001-6322 (Print)
IS  - 0001-6322 (Linking)
VI  - 74
IP  - 3
DP  - 1987
TI  - Blood-brain barrier leakage and brain edema in stroke-prone spontaneously 
      hypertensive rats. Effect of chronic sympathectomy and low protein/high salt 
      diet.
PG  - 259-68
AB  - Brain edema associated with severe chronic hypertension was studied in 
      stroke-prone spontaneously hypertensive rats (SHRSP), 5 to 9 months of age. 
      Blood-brain barrier (BBB) leakage sites and intracerebral spreading pathways for 
      plasma proteins were delineated by an intravenously (i.v.) injected exogenous dye 
      tracer (Evans blue), known to form a complex with albumin in blood, and by 
      immunohistochemical visualization of extravasated endogenous plasma proteins. The 
      tissue content of edema fluid was estimated by measuring the specific gravity of 
      selected brain regions, stained or unstained by the tracer dye, on a 
      bromobenzene-kerosene gradient column. Multifocal BBB leakage sites were 
      macroscopically detected within the cerebral cortex and the deep gray matter 
      after i.v. circulation of Evans blue-albumin for 30 min. After 24 h of i.v. 
      circulation the dye tracer had spread not only locally in the gray matter but 
      also into the adjacent white matter, where it was widely distributed. 
      Immunohistochemically visualized plasma proteins showed similar distribution. 
      Unilateral superior cervical ganglionectomy performed at 4 weeks of age neither 
      increased the incidence of major BBB opening to Evans blue-albumin nor altered 
      the specific gravity of the ipsilateral cerebral hemisphere in grown-up SHRSP, 
      furthermore, the blood pressure remained unchanged. The lack of significant 
      effect on BBB function may possibly be attributed to the extensive reinnervation 
      of the cerebral arteries, verified in the grown-up SHRSP using the Falck-Hillarp 
      fluorescence method for visualization of catecholaminergic nerve fibers. In SHRSP 
      raised on a low-protein and high-salt diet the mean arterial blood pressure was 
      212 mm Hg compared to 195 mm Hg in controls (P less than 0.05) and the incidence 
      of BBB opening was 72% compared to 25% in controls (P less than 0.05). After 24 h 
      of i.v. circulation of Evans blue-albumin, brain regions stained by the dye 
      tracer showed significantly reduced specific gravity (P less than 0.001), while 
      unstained regions had normal values. Thus the brain edema fluid spread, as 
      revealed by specific gravity measurements, corresponded to the intracerebral 
      distribution of extravasated plasma proteins.
FAU - Fredriksson, K
AU  - Fredriksson K
AD  - Department of Neurology, University of Lund, Sweden.
FAU - Kalimo, H
AU  - Kalimo H
FAU - Westergren, I
AU  - Westergren I
FAU - Kahrstrom, J
AU  - Kahrstrom J
FAU - Johansson, B B
AU  - Johansson BB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Acta Neuropathol
JT  - Acta neuropathologica
JID - 0412041
RN  - 0 (Blood Proteins)
RN  - 0 (Dietary Proteins)
RN  - 0 (Sodium, Dietary)
SB  - IM
MH  - Animals
MH  - Blood Proteins/*physiology
MH  - *Blood-Brain Barrier/drug effects
MH  - Brain/*physiopathology
MH  - Brain Edema/*physiopathology
MH  - Cerebral Arteries/innervation
MH  - Cerebrovascular Disorders/physiopathology
MH  - Dietary Proteins/administration & dosage/metabolism
MH  - Female
MH  - Hypertension/physiopathology
MH  - Male
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Sodium, Dietary/administration & dosage
MH  - Specific Gravity
MH  - Sympathectomy
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1007/BF00688190 [doi]
PST - ppublish
SO  - Acta Neuropathol. 1987;74(3):259-68. doi: 10.1007/BF00688190.