PMID- 36737470
OWN - NLM
STAT- MEDLINE
DCOM- 20230207
LR  - 20230317
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Feb 3
TI  - Immature and mature bone marrow-derived dendritic cells exhibit distinct 
      intracellular mechanical properties.
PG  - 1967
LID - 10.1038/s41598-023-28625-w [doi]
LID - 1967
AB  - Dendritic cells (DCs) patrol the organism at an immature stage to detect the 
      presence of pathogens. Once activated, these mature DCs reach the lymph nodes to 
      activate antigen-specific T lymphocytes and thus initiate an adaptative immune 
      response to control the pathogen. The migration of both immature and mature DCs 
      is a key process for their optimal function. DC migration requires transit 
      through narrow constrictions that is allowed by their high local and global 
      deformation capabilities. In addition to cytoplasmic changes, the nucleus 
      mechanical properties also have a major impact for cellular migration and 
      motility. Yet, nucleus intracellular mobility of dendritic cells or its variation 
      upon maturation have not been investigated. Our study defines the biophysical 
      phenotypic variations of dendritic cells upon maturation using interferometric 
      deformability cytometry. This method characterizes different cellular mechanical 
      properties, such as elongation and nucleus offset, by assessing the refractive 
      index spatial distribution of shear-induced deformed cells. By using these 
      parameters, our data suggest that in vitro bone marrow derived dendritic cell 
      (BMDC) maturation induces cell stiffening and reduces nucleus mobility, allowing 
      to distinguish immature and mature dendritic cells. Overall, our method provides 
      insights on intracellular mechanical properties of two dendritic cell states.
CI  - (c) 2023. The Author(s).
FAU - Leblanc-Hotte, Antoine
AU  - Leblanc-Hotte A
AD  - Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital Research Centre, 
      Montreal, QC, Canada.
AD  - Departement de Microbiologie, Infectiologie et Immunologie, Universite de 
      Montreal, Montreal, QC, Canada.
AD  - Department of Engineering Physics, Polytechnique Montreal, Montreal, QC, Canada.
FAU - Audiger, Cindy
AU  - Audiger C
AD  - Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital Research Centre, 
      Montreal, QC, Canada.
AD  - Departement de Microbiologie, Infectiologie et Immunologie, Universite de 
      Montreal, Montreal, QC, Canada.
FAU - Chabot-Roy, Genevieve
AU  - Chabot-Roy G
AD  - Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital Research Centre, 
      Montreal, QC, Canada.
FAU - Lombard-Vadnais, Felix
AU  - Lombard-Vadnais F
AD  - Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital Research Centre, 
      Montreal, QC, Canada.
FAU - Delisle, Jean-Sebastien
AU  - Delisle JS
AD  - Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital Research Centre, 
      Montreal, QC, Canada.
AD  - Departement de Medecine, Universite de Montreal, Montreal, QC, Canada.
FAU - Peter, Yves-Alain
AU  - Peter YA
AD  - Department of Engineering Physics, Polytechnique Montreal, Montreal, QC, Canada. 
      yves-alain.peter@polymtl.ca.
FAU - Lesage, Sylvie
AU  - Lesage S
AD  - Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital Research Centre, 
      Montreal, QC, Canada. sylvie.lesage@umontreal.ca.
AD  - Departement de Microbiologie, Infectiologie et Immunologie, Universite de 
      Montreal, Montreal, QC, Canada. sylvie.lesage@umontreal.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230203
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Cell Differentiation
MH  - *Bone Marrow
MH  - *T-Lymphocytes
MH  - Cells, Cultured
MH  - Dendritic Cells
PMC - PMC9898242
COIS- The authors declare no competing interests.
EDAT- 2023/02/04 06:00
MHDA- 2023/02/08 06:00
PMCR- 2023/02/03
CRDT- 2023/02/03 23:25
PHST- 2022/10/31 00:00 [received]
PHST- 2023/01/20 00:00 [accepted]
PHST- 2023/02/03 23:25 [entrez]
PHST- 2023/02/04 06:00 [pubmed]
PHST- 2023/02/08 06:00 [medline]
PHST- 2023/02/03 00:00 [pmc-release]
AID - 10.1038/s41598-023-28625-w [pii]
AID - 28625 [pii]
AID - 10.1038/s41598-023-28625-w [doi]
PST - epublish
SO  - Sci Rep. 2023 Feb 3;13(1):1967. doi: 10.1038/s41598-023-28625-w.