PMID- 36738873
OWN - NLM
STAT- MEDLINE
DCOM- 20230307
LR  - 20230314
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 666
DP  - 2023 Apr 1
TI  - Establishment of matrix metalloproteinase 3 time-resolved immunoassay and some 
      potential clinical applications.
PG  - 115072
LID - S0003-2697(23)00037-4 [pii]
LID - 10.1016/j.ab.2023.115072 [doi]
AB  - AIM: To develop a highly sensitive time-resolved fluorescence immunoassay (TRFIA) 
      for the detection of serum matrix metalloproteinase-3 (MMP-3) and to assess 
      MMP-3's clinical value in patients with colorectal cancer (CRC).st. METHODS: 
      MMP-3 levels were established using the double antibody sandwich technique. The 
      MMP-3 TRFIA technique was developed and optimized, and its linearity, 
      sensitivity, accuracy, specificity, and recovery were assessed. Then, serum 
      concentrations in healthy individuals and patients with CRC were determined by 
      MMP-3 TRFIA. RESULTS: The linear range of MMP-3 TRFIA was 0.73-500 ng/mL. MMP-3 
      TRFIA had an intra-batch precision range of 2.16%-7.10% percent and an 
      inter-batch precision range of 3.99%-11.21%. MMP-3, tumor-associated trypsinogen 
      2, and AFP had no cross reaction.The recovery is between 90% and 110%, and had no 
      serum interference. Patients with CRC had serum MMP-3 levels 
      (73.95 +/- 78.43 ng/mL) that were considerably higher than those of healthy 
      individuals (21.45 +/- 11.12 ng/mL), and those with metastasis had serum MMP-3 
      levels (95.89 +/- 76.21 ng/mL) that were considerably higher than those of patients 
      without metastasis (52.74 +/- 47.25 ng/mL). CONCLUSIONS: A highly sensitive MMP-3 
      TRFIA assay was successfully developed, and serum MMP-3 may be associated with 
      CRC invasion and metastasis. Therefore, MMP-3 can be used in the auxiliary 
      diagnosis of CRC.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Fu, Yulin
AU  - Fu Y
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, China.
FAU - Chen, Xindong
AU  - Chen X
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Hong, Jianfeng
AU  - Hong J
AD  - Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, China.
FAU - Qin, Yuan
AU  - Qin Y
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Zhou, Zixuan
AU  - Zhou Z
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Zhou, Xiumei
AU  - Zhou X
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Wang, Yigang
AU  - Wang Y
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Zhou, Jingnan
AU  - Zhou J
AD  - Zhejiang Cancer Hospital, Hangzhou, China.
FAU - Fang, Hongming
AU  - Fang H
AD  - Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, China.
FAU - Liu, Pengfei
AU  - Liu P
AD  - The Jiangyin Clinical College of Xuzhou Medical University, Jiangyin, China.
FAU - Huang, Biao
AU  - Huang B
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China. Electronic address: jswxhb@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230203
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (Antibodies)
SB  - IM
MH  - Humans
MH  - *Matrix Metalloproteinase 3
MH  - *Fluoroimmunoassay/methods
MH  - Serum
MH  - Antibodies
OTO - NOTNLM
OT  - Colorectal cancer
OT  - Diagnosis
OT  - Double antibody sandwich
OT  - MMP-3
OT  - TRFIA
EDAT- 2023/02/05 06:00
MHDA- 2023/03/08 06:00
CRDT- 2023/02/04 19:24
PHST- 2022/08/26 00:00 [received]
PHST- 2023/01/01 00:00 [revised]
PHST- 2023/02/01 00:00 [accepted]
PHST- 2023/02/05 06:00 [pubmed]
PHST- 2023/03/08 06:00 [medline]
PHST- 2023/02/04 19:24 [entrez]
AID - S0003-2697(23)00037-4 [pii]
AID - 10.1016/j.ab.2023.115072 [doi]
PST - ppublish
SO  - Anal Biochem. 2023 Apr 1;666:115072. doi: 10.1016/j.ab.2023.115072. Epub 2023 Feb 
      3.