PMID- 36740146
OWN - NLM
STAT- MEDLINE
DCOM- 20230227
LR  - 20230320
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 462
DP  - 2023 Mar 1
TI  - Ring finger protein 10 improves pirarubicin-induced cardiac inflammation by 
      regulating the AP-1/Meox2 signaling pathway.
PG  - 116411
LID - S0041-008X(23)00049-2 [pii]
LID - 10.1016/j.taap.2023.116411 [doi]
AB  - OBJECTIVES: Pirarubicin (THP) is widely used in clinical antitumor therapy, but 
      its cardiotoxicity seriously affects the therapeutic effect in patients. In the 
      study, we investigated the role of ring finger protein 10 (RNF10) in 
      cardiotoxicity induced by THP. MATERIALS AND METHODS: A cardiac toxicity model in 
      Sprague-Dawley (SD) rats induced by THP was established. Changes in diet, weight, 
      electrocardiogram (ECG), and echocardiography were observed. Serum levels of 
      brain natriuretic peptide (BNP), creatine kinase MB (CK-MB), cardiac troponin T 
      (cTnT), and lactate dehydrogenase (LDH) were measured. The expression of RNF10 in 
      myocardium was observed by immunohistochemistry. The expressions of RNF10, 
      activator protein-1 (AP-1), mesenchyme homeobox 2 (Meox2), total nuclear factor 
      (NF)-kappaB p65 (T-P65), phosphorylated NF-kappaB p65 (PP65), monocyte chemoattractant 
      protein-1 (MCP-1), tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and mature 
      IL-1beta were detected by Western blot. A THP-induced H9c2 myocardial cell injury 
      model was established. RNF10 was downregulated or overexpressed by RNF10 siRNA 
      and a RNF10 lentiviral vector, respectively. Then, cell viability was measured. 
      The expression of RNF10 in H9c2 cells was observed by immunofluorescence. All of 
      the above signaling pathways were verified by Western blots. FINDINGS: THP caused 
      a series of cardiotoxic manifestations in SD rats. Our studies suggested that THP 
      caused cardiac inflammation by inhibiting the expression of RNF10, while 
      overexpression of RNF10 antagonized the cardiotoxicity induced by THP. 
      SIGNIFICANCE: Our study showed RNF10 improved THP-induced cardiac inflammation by 
      regulating the AP-1/Meox2 signaling pathway. RNF10 may be a new target to treat 
      THP-induced cardiotoxicity.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Duan, Liang
AU  - Duan L
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Tang, Heng
AU  - Tang H
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Lan, Ying
AU  - Lan Y
AD  - Department of Critical Care Medicine, Affiliated Hospital of Chengdu University, 
      Chengdu, Sichuan, China.
FAU - Shi, Hongwei
AU  - Shi H
AD  - Department of Radiation Oncology, Hubei Cancer Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China; Department of 
      Oncology, Renmin Hospital of Wuhan University, Wuhan, China.
FAU - Pu, Peng
AU  - Pu P
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - He, Quan
AU  - He Q
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China. Electronic address: hequan822@aliyun.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230203
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - D58G680W0G (pirarubicin)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (RNF10 protein, rat)
RN  - 0 (Carrier Proteins)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Transcription Factor AP-1/metabolism
MH  - *Cardiotoxicity/etiology
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - NF-kappa B/metabolism
MH  - Arrhythmias, Cardiac
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Interleukin-6/metabolism
MH  - Inflammation/pathology
MH  - Carrier Proteins/metabolism
MH  - Nerve Tissue Proteins/metabolism
OTO - NOTNLM
OT  - AP-1
OT  - Cardiac inflammation
OT  - Meox2
OT  - Pirarubicin
OT  - RNF10
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could influence the 
      work reported in this paper.
EDAT- 2023/02/06 06:00
MHDA- 2023/03/03 06:00
CRDT- 2023/02/05 19:28
PHST- 2022/09/21 00:00 [received]
PHST- 2023/01/20 00:00 [revised]
PHST- 2023/01/31 00:00 [accepted]
PHST- 2023/02/06 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/05 19:28 [entrez]
AID - S0041-008X(23)00049-2 [pii]
AID - 10.1016/j.taap.2023.116411 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2023 Mar 1;462:116411. doi: 10.1016/j.taap.2023.116411. 
      Epub 2023 Feb 3.