PMID- 36746436
OWN - NLM
STAT- MEDLINE
DCOM- 20230316
LR  - 20230316
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 134
IP  - 3
DP  - 2023 Mar 1
TI  - Respiratory depression driven by membrane damage as a mechanism for linalool to 
      inhibit Pseudomonas lundensis and its preservation potential for beef.
LID - lxad023 [pii]
LID - 10.1093/jambio/lxad023 [doi]
AB  - AIMS: This study aimed to investigate the mechanism of linalool against 
      Pseudomonas lundensis and its application on beef. METHODS AND RESULTS: Field 
      emission scanning electron microscopy found that linalool exerted antibacterial 
      activity with a minimum inhibitory concentration (MIC) of 1.5 ml l-1 by 
      disrupting cell structure. Loss of cell membrane integrity was monitored due to 
      leakage of nucleic acids and K+. In addition, respiratory depression appeared in 
      Ps. lundensis based on inhibition of enzyme activities including hexokinase (HK), 
      glucose 6-phosphate dehydrogenase (G6PDH), phosphofructokinase (PFK), pyruvate 
      kinase (PK), pyruvate dehydrogenase (PDH), citrate synthase (CS), succinate 
      dehydrogenase (SDH), and malate dehydrogenase (MDH). Subsequently, energy 
      limitation also occurred according to the decrease in ATP content and ATPase 
      activity. Molecular docking confirmed that linalool can combine with enzymes in 
      cell wall (ddlB) and energy synthesis (AtpD) pathways to exert antibacterial 
      effect. Of note, linalool has advantages for beef preservation by delaying 
      quality changes including pH, total volatile basic nitrogen (TVB-N) and total 
      viable count (TVC). CONCLUSIONS: Linalool has significant inhibitory effect on 
      Ps. lundensis, and respiratory depression driven by membrane damage is the main 
      inhibitory mechanism.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of Applied 
      Microbiology International.
FAU - He, Rongrong
AU  - He R
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
FAU - Chen, Haiming
AU  - Chen H
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
FAU - Chen, Weijun
AU  - Chen W
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
AD  - Chunguang Agro-product Processing Institute, Wenchang 571333, China.
FAU - Zhang, Ming
AU  - Zhang M
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
FAU - Pei, Jianfei
AU  - Pei J
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
FAU - Chen, Wenxue
AU  - Chen W
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
FAU - Zhong, Qiuping
AU  - Zhong Q
AD  - Hainan University-HSF/LWL Collaborative Innovation Laboratory, School of Food 
      Science and Engineering, Hainan University, 58 People Road, Haikou 570228, PR 
      China.
LA  - eng
GR  - 32160591/Natural Science Foundation of China/
GR  - 320RC515/Hainan Provincial Natural Science Foundation of China/
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - D81QY6I88E (linalool)
RN  - 0 (Anti-Bacterial Agents)
RN  - Pseudomonas lundensis
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Molecular Docking Simulation
MH  - *Anti-Bacterial Agents/pharmacology
MH  - *Respiratory Insufficiency
OTO - NOTNLM
OT  - Pseudomonas lundensis
OT  - ATP
OT  - beef
OT  - enzyme activity
OT  - linalool
OT  - molecular docking
EDAT- 2023/02/07 06:00
MHDA- 2023/03/17 06:00
CRDT- 2023/02/06 20:13
PHST- 2022/11/01 00:00 [received]
PHST- 2023/01/11 00:00 [revised]
PHST- 2023/02/05 00:00 [accepted]
PHST- 2023/02/07 06:00 [pubmed]
PHST- 2023/03/17 06:00 [medline]
PHST- 2023/02/06 20:13 [entrez]
AID - 7028771 [pii]
AID - 10.1093/jambio/lxad023 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2023 Mar 1;134(3):lxad023. doi: 10.1093/jambio/lxad023.