PMID- 36747698
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230731
DP  - 2023 Jan 23
TI  - Three-dimensional imaging and quantification of mouse ovarian follicles via 
      optical coherence tomography.
LID - 2023.01.23.525192 [pii]
LID - 10.1101/2023.01.23.525192 [doi]
AB  - Ovarian tissue cryopreservation has been successfully applied worldwide for 
      fertility preservation. Correctly selecting the ovarian tissue with high follicle 
      loading for freezing and reimplantation increases the likelihood of restoring 
      ovarian function, but it is a challenging process. In this work, we explore the 
      use of three-dimensional spectral-domain optical coherence tomography (SD-OCT) to 
      identify different follicular stages, especially primary follicles, compare the 
      identifications with H&E images, and measure the size and age-related follicular 
      density distribution differences in mice ovaries. We use the thickness of the 
      layers of granulosa cells to differentiate primordial and primary follicles from 
      secondary follicles. The measured dimensions and age-related follicular 
      distribution agree well with histological images and physiological aging. 
      Finally, we apply attenuation coefficient map analyses to significantly improve 
      the image contrast and the contrast-to-noise ratio (p < 0.001), facilitating 
      follicle identification and quantification. We conclude that SD-OCT is a 
      promising method to noninvasively evaluate ovarian follicles.
FAU - Amaral, Marcello Magri
AU  - Amaral MM
AD  - Biomedical Engineering Department, Washington University in St Louis, 1 Brookings 
      Dr, St Louis, MO, USA.
AD  - Universidade Brasil, Rua Carolina Fonseca 235, Sao Paulo, SP, BR.
FAU - Sun, Aixia
AU  - Sun A
AD  - Precision Health Program, Michigan State University, East Lansing, MI, USA.
AD  - Department of Radiology, College of Human Medicine, Michigan State University, 
      East Lansing, MI, USA.
FAU - Li, Yilin
AU  - Li Y
AD  - Biomedical Engineering Department, Washington University in St Louis, 1 Brookings 
      Dr, St Louis, MO, USA.
FAU - Ren, Chao
AU  - Ren C
AD  - Biomedical Engineering Department, Washington University in St Louis, 1 Brookings 
      Dr, St Louis, MO, USA.
FAU - Truong, Anh Blue
AU  - Truong AB
AD  - Biomedical Engineering Department, Washington University in St Louis, 1 Brookings 
      Dr, St Louis, MO, USA.
FAU - Nigam, Saumya
AU  - Nigam S
AD  - Precision Health Program, Michigan State University, East Lansing, MI, USA.
AD  - Department of Radiology, College of Human Medicine, Michigan State University, 
      East Lansing, MI, USA.
FAU - Jiao, Zexu
AU  - Jiao Z
AD  - Department of Obstetrics and Gynecology, University of Texas Southwestern Medical 
      Center, Dallas, Tx, USA.
FAU - Wang, Ping
AU  - Wang P
AD  - Precision Health Program, Michigan State University, East Lansing, MI, USA.
AD  - Department of Radiology, College of Human Medicine, Michigan State University, 
      East Lansing, MI, USA.
FAU - Zhou, Chao
AU  - Zhou C
AD  - Biomedical Engineering Department, Washington University in St Louis, 1 Brookings 
      Dr, St Louis, MO, USA.
LA  - eng
GR  - R01 EB025209/EB/NIBIB NIH HHS/United States
PT  - Preprint
DEP - 20230123
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
UIN - Biomed Opt Express. 2023 Jun 12;14(7):3213-3224. PMID: 37497517
PMC - PMC9900855
EDAT- 2023/02/08 06:00
MHDA- 2023/02/08 06:01
PMCR- 2023/02/06
CRDT- 2023/02/07 01:56
PHST- 2023/02/07 01:56 [entrez]
PHST- 2023/02/08 06:00 [pubmed]
PHST- 2023/02/08 06:01 [medline]
PHST- 2023/02/06 00:00 [pmc-release]
AID - 2023.01.23.525192 [pii]
AID - 10.1101/2023.01.23.525192 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Jan 23:2023.01.23.525192. doi: 
      10.1101/2023.01.23.525192.