PMID- 36750341
OWN - NLM
STAT- MEDLINE
DCOM- 20230209
LR  - 20230209
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 52
IP  - 1
DP  - 2023 Jan
TI  - [Determination of 25-hydroxyvitamin D in serum by pre-column 
      derivatization-stable isotope labeling-ultra performance liquid 
      chromatography-quadrupole electrostatic field orbitrap high-resolution mass 
      spectrometry].
PG  - 129-135
LID - 10.19813/j.cnki.weishengyanjiu.2023.01.022 [doi]
AB  - OBJECTIVE: To establish a rapid and accurate method for the determination of 
      25-hydroxyvitamin D_3(25(OH)D_3) and 25-hydroxyvitamin D_2(25(OH)D_2)in serum by 
      pre-column derivatization with stable isotope labeling and ultra performance 
      liquid chromatography-quadrupole electrostatic field orbitrap high-resolution 
      mass spectrometry, which could be used to diagnose vitamin deficiency and to 
      assess the nutritional status of vitamin D in the population. METHODS: The serum 
      samples with isotopic internal standard were extracted by mixed extraction 
      solvent(ethyl acetateratiohexane = 2ratio1, V/V), centrifuged and dried by the nitrogen 
      blowing, and derivatized with 4-phenyl-1, 2, 4-triazoline-3, 5-dione(PTAD). The 
      reaction products were separated on a BEH C_(18) column(2.1 mmx50 mm, 1.7 mum), 
      and eluted with a 0.1% formic acid/water solution-acetonitrile gradient. The mass 
      spectrometry was performed in positive electrospray ionization(ESI~+) and 
      parallel reaction monitoring(PRM) for the detection of the targets, and 
      quantified by isotope internal standard. RESULTS: The limits of detection both 
      for 25(OH)D_3 and 25(OH)D_2 were 0.01 ng/mL and the limits of quantification were 
      0.03 ng/mL. The concentration series of 25(OH)D_2 ranged from 0.5 to 40.0 ng/mL 
      and the concentration series of 25(OH)D_3 ranged from 2.5 to 200.0 ng/mL. The 
      recoveries(n=5) were 95.7%-101.3% for 25(OH)D_3, and 98.7%-108.6% for 25(OH)D_2, 
      with the relative standard deviations(RSDs) of 0.5%-4.9% and 2.2%-3.4%, 
      respectively. The accuracy of the determination of 25(OH)D_3 and 25(OH)D_2 in 
      Level 2 serum of the 25(OH)D standard reference material NIST SRM 972a was 104.8% 
      and 94.9%, respectively. The M(P25, P75)serum levels of 25(OH)D_3 and 25(OH)D_2 
      for 116 pregnant women at the first trimester from Beijing were 25.7(20.8, 32.6) 
      ng/mL and 0.8(0.4, 1.1) ng/mL, respectively. CONCLUSION: This method is highly 
      sensitive, qualitatively accurate and suitable for evaluation and monitoring the 
      nutritional status of vitamin D in the population.
FAU - Han, Feng
AU  - Han F
AD  - NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition 
      and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, 
      China.
FAU - Wang, Yuxin
AU  - Wang Y
AD  - China National Center for Food Safety Risk Assessment, Beijing 100021, China.
FAU - Li, Jingguang
AU  - Li J
AD  - China National Center for Food Safety Risk Assessment, Beijing 100021, China.
FAU - Li, Zizi
AU  - Li Z
AD  - NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition 
      and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, 
      China.
FAU - Mu, Di
AU  - Mu D
AD  - NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition 
      and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, 
      China.
FAU - He, Li
AU  - He L
AD  - NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition 
      and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, 
      China.
FAU - Zhang, Jian
AU  - Zhang J
AD  - NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition 
      and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - A288AR3C9H (25-hydroxyvitamin D)
RN  - 1406-16-2 (Vitamin D)
RN  - P6YZ13C99Q (Calcifediol)
RN  - 0 (Vitamins)
SB  - IM
MH  - Pregnancy
MH  - Female
MH  - Humans
MH  - Chromatography, High Pressure Liquid
MH  - Static Electricity
MH  - Isotope Labeling
MH  - *Tandem Mass Spectrometry/methods
MH  - *Vitamin D
MH  - Calcifediol
MH  - Chromatography, Liquid/methods
MH  - Vitamins
OTO - NOTNLM
OT  - 25-hydroxyvitamin D_2
OT  - 25-hydroxyvitamin D_3
OT  - derivatization
OT  - ultra-performance liquid chromatography-quadrupole electrostatic field orbitrap 
      high-resolution mass spectrometry
EDAT- 2023/02/08 06:00
MHDA- 2023/02/10 06:00
CRDT- 2023/02/07 21:13
PHST- 2023/02/07 21:13 [entrez]
PHST- 2023/02/08 06:00 [pubmed]
PHST- 2023/02/10 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2023.01.022 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2023 Jan;52(1):129-135. doi: 
      10.19813/j.cnki.weishengyanjiu.2023.01.022.