PMID- 36750663
OWN - NLM
STAT- MEDLINE
DCOM- 20230209
LR  - 20230209
IS  - 2399-3642 (Electronic)
IS  - 2399-3642 (Linking)
VI  - 6
IP  - 1
DP  - 2023 Feb 7
TI  - Complexin-1 regulated assembly of single neuronal SNARE complex revealed by 
      single-molecule optical tweezers.
PG  - 155
LID - 10.1038/s42003-023-04506-w [doi]
LID - 155
AB  - The dynamic assembly of the Synaptic-soluble N-ethylmaleimide-sensitive factor 
      Attachment REceptor (SNARE) complex is crucial to understand membrane fusion. 
      Traditional ensemble study meets the challenge to dissect the dynamic assembly of 
      the protein complex. Here, we apply minute force on a tethered protein complex 
      through dual-trap optical tweezers and study the folding dynamics of SNARE 
      complex under mechanical force regulated by complexin-1 (CpxI). We reconstruct 
      the clamp and facilitate functions of CpxI in vitro and identify different 
      interplay mechanism of CpxI fragment binding on the SNARE complex. Specially, 
      while the N-terminal domain (NTD) plays a dominant role of the facilitate 
      function, CTD is mainly related to clamping. And the mixture of 1-83aa and CTD of 
      CpxI can efficiently reconstitute the inhibitory signal identical to that the 
      full-length CpxI functions. Our observation identifies the important chaperone 
      role of the CpxI molecule in the dynamic assembly of SNARE complex under 
      mechanical tension, and elucidates the specific function of each fragment of CpxI 
      molecules in the chaperone process.
CI  - (c) 2023. The Author(s).
FAU - Hao, Tongrui
AU  - Hao T
AUID- ORCID: 0000-0002-4237-5666
AD  - State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and 
      Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of 
      Science, Shanghai, 200031, China. haotongrui2018@sibcb.ac.cn.
AD  - University of Chinese Academy of Sciences, Beijing, 200049, China. 
      haotongrui2018@sibcb.ac.cn.
FAU - Feng, Nan
AU  - Feng N
AD  - State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and 
      Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of 
      Science, Shanghai, 200031, China.
AD  - University of Chinese Academy of Sciences, Beijing, 200049, China.
FAU - Gong, Fan
AU  - Gong F
AD  - National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai 
      Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, 
      China.
FAU - Yu, Yang
AU  - Yu Y
AD  - National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai 
      Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, 
      China.
FAU - Liu, Jiaquan
AU  - Liu J
AUID- ORCID: 0000-0001-6206-3742
AD  - State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and 
      Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of 
      Science, Shanghai, 200031, China. liujiaquan@sibcb.ac.cn.
FAU - Ren, Yu-Xuan
AU  - Ren YX
AUID- ORCID: 0000-0002-4641-3148
AD  - Institute for Translational Brain Research, Shanghai Medical College, Fudan 
      University, Shanghai, 200032, China. yxren@fudan.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230207
PL  - England
TA  - Commun Biol
JT  - Communications biology
JID - 101719179
RN  - 0 (SNARE Proteins)
SB  - IM
MH  - *SNARE Proteins/chemistry/metabolism
MH  - *Optical Tweezers
MH  - Membrane Fusion
PMC - PMC9905088
COIS- The authors declare no competing interests.
EDAT- 2023/02/08 06:00
MHDA- 2023/02/10 06:00
PMCR- 2023/02/07
CRDT- 2023/02/07 23:50
PHST- 2022/02/27 00:00 [received]
PHST- 2023/01/19 00:00 [accepted]
PHST- 2023/02/07 23:50 [entrez]
PHST- 2023/02/08 06:00 [pubmed]
PHST- 2023/02/10 06:00 [medline]
PHST- 2023/02/07 00:00 [pmc-release]
AID - 10.1038/s42003-023-04506-w [pii]
AID - 4506 [pii]
AID - 10.1038/s42003-023-04506-w [doi]
PST - epublish
SO  - Commun Biol. 2023 Feb 7;6(1):155. doi: 10.1038/s42003-023-04506-w.