PMID- 36751854
OWN - NLM
STAT- MEDLINE
DCOM- 20230413
LR  - 20230413
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 37
IP  - 4
DP  - 2023 Apr
TI  - Narirutin exerts anti-inflammatory activity by inhibiting NLRP3 inflammasome 
      activation in macrophages.
PG  - 1293-1308
LID - 10.1002/ptr.7686 [doi]
AB  - Citrus peel has long been used in traditional medicine in Asia to treat common 
      cold, dyspepsia, cough, and phlegm. Narirutin-a flavanone-7-O-glycoside-is the 
      major flavonoid in citrus peel, and has anti-oxidative, anti-allergic, and 
      anti-inflammatory activities. However, the anti-inflammatory mechanism of 
      narirutin has not been fully elucidated. This study is aimed to investigate the 
      effects of narirutin on the Nod-like receptor protein 3 (NLRP3)-mediated 
      inflammatory response in vitro and in vivo, and determine the underlying 
      mechanism. THP-1 differentiated macrophages and bone marrow-derived macrophages 
      (BMDMs) were used for in vitro experiments, while dextran sulfate sodium 
      (DSS)-induced colitis and alum-induced peritonitis mouse models were constructed 
      to test inflammation in vivo. Narirutin suppressed secretion of interleukin 
      (IL)-1beta and pyroptosis in lipopolysaccharide (LPS)/ATP-stimulated macrophages. 
      Narirutin decreased the expression of NLRP3 and IL-1beta in the LPS-priming step 
      through inhibition of NF-kappaB, MAPK and PI3K /AKT signaling pathways. Narirutin 
      inhibited NLRP3-ASC interaction to suppress NLRP3 inflammasome assembly. 
      Furthermore, oral administration of narirutin (300 mg/kg) alleviated inflammation 
      symptoms in mice with peritonitis and colitis. These results suggest that 
      narirutin exerts its anti-inflammatory activity by suppressing NLRP3 inflammasome 
      activation via inhibition of the NLRP3 inflammasome priming processes and 
      NLRP3-ASC interaction in macrophages.
CI  - (c) 2023 John Wiley & Sons Ltd.
FAU - Ri, Myong Hak
AU  - Ri MH
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
AD  - Faculty of Life Science, Kim Il Sung University, Pyongyang, Democratic People's 
      Republic of Korea.
FAU - Li, Ming Yue
AU  - Li MY
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
FAU - Xing, Yue
AU  - Xing Y
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
FAU - Zuo, Hong Xiang
AU  - Zuo HX
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
FAU - Li, Guangyi
AU  - Li G
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
FAU - Li, Chengcheng
AU  - Li C
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
FAU - Ma, Juan
AU  - Ma J
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
FAU - Jin, Xuejun
AU  - Jin X
AUID- ORCID: 0000-0001-6998-2602
AD  - Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of 
      Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian 
      University, Yanji, China.
LA  - eng
GR  - 81760657/National Natural Science Foundation of China/
GR  - 602021041/Yanbian University Doctoral Research Fund Project/
GR  - YDZJ2022101ZYTS180/The Jilin Province Science and Technology Development Plan 
      Project/
GR  - YDZJ202201ZYTS169/The Jilin Province Science and Technology Development Plan 
      Project/
GR  - YDZJ202201ZYTS272/The Jilin Province Science and Technology Development Plan 
      Project/
GR  - JJKH20220561KJ/the Education Department of Jilin Province Project/
GR  - ydbq202113/Yanbian University Doctoral Research Fund Project/
GR  - ydbq202216/Yanbian University Doctoral Research Fund Project/
GR  - 111Project,D18012/the Higher Education Discipline Innovation Project/
PT  - Journal Article
DEP - 20230207
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 06M5EAT0YC (narirutin)
RN  - 0 (NLR Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Flavanones)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Nlrp3 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Inflammasomes
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - NLR Proteins/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages
MH  - *Flavanones/pharmacology
MH  - *Colitis/chemically induced
MH  - Inflammation/metabolism
MH  - Anti-Inflammatory Agents/pharmacology
MH  - *Peritonitis/metabolism
OTO - NOTNLM
OT  - DSS-induced colitis
OT  - NLRP3 inflammasome
OT  - alum-induced peritonitis
OT  - citrus peel
OT  - inflammation
OT  - narirutin
EDAT- 2023/02/09 06:00
MHDA- 2023/04/13 06:42
CRDT- 2023/02/08 02:42
PHST- 2022/10/18 00:00 [revised]
PHST- 2022/07/30 00:00 [received]
PHST- 2022/10/27 00:00 [accepted]
PHST- 2023/04/13 06:42 [medline]
PHST- 2023/02/09 06:00 [pubmed]
PHST- 2023/02/08 02:42 [entrez]
AID - 10.1002/ptr.7686 [doi]
PST - ppublish
SO  - Phytother Res. 2023 Apr;37(4):1293-1308. doi: 10.1002/ptr.7686. Epub 2023 Feb 7.