PMID- 36752573 OWN - NLM STAT- MEDLINE DCOM- 20230308 LR - 20230320 IS - 1097-0215 (Electronic) IS - 0020-7136 (Linking) VI - 152 IP - 9 DP - 2023 May 1 TI - Convergent dysbiosis of upper aerodigestive microbiota between patients with esophageal and oral cavity squamous cell carcinoma. PG - 1903-1915 LID - 10.1002/ijc.34460 [doi] AB - The bidirectional association between primary esophageal squamous cell carcinoma (ESCC) and oral cavity squamous cell carcinoma (OSCC) suggests common risk factors and oncogenic molecular processes but it is unclear whether these two cancers display similar patterns of dysbiosis in their upper aerodigestive microbiota (UADM). We conducted a case-control study to characterize the microbial communities in esophageal lavage samples from 49 ESCC patients and oral rinse samples from 91 OSCC patients using 16S rRNA V3-V4 amplicon sequencing. Compared with their respective non-SCC controls from the same anatomical sites, 32 and 45 discriminative bacterial genera were detected in ESCC and OSCC patients, respectively. Interestingly, 20 of them were commonly enriched or depleted in both types of cancer, suggesting a convergent niche adaptation of upper aerodigestive SCC-associated bacteria that may play important roles in the pathogenesis of malignancies. Notably, Fusobacterium, Selenomonas, Peptoanaerobacter and Peptostreptococcus were enriched in both ESCC and OSCC, whereas Streptococcus and Granulicatelia were commonly depleted. We further identified Fusobacterium nucleatum as the most abundant species enriched in the upper aerodigestive SCC microenvironment, and the higher relative abundances of Selenomonas danae and Treponema maroon were positively correlated with smoking. In addition, predicted functional analysis revealed several depleted (eg, lipoic acid and pyruvate metabolism) and enriched (eg, RNA polymerase and nucleotide excision repair) pathways common to both cancers. Our findings reveal a convergent dysbiosis in the UADM between patients with ESCC and OSCC, suggesting a shared niche adaptation of host-microbiota interactions in the pathogenesis of upper aerodigestive tract malignancies. CI - (c) 2023 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC. FAU - Zhu, Hengyan AU - Zhu H AUID- ORCID: 0000-0002-2040-9036 AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Yip, Hon Chi AU - Yip HC AUID- ORCID: 0000-0003-1041-9668 AD - Department of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Cheung, Man Kit AU - Cheung MK AUID- ORCID: 0000-0002-2764-2113 AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Chan, Hiu Ching AU - Chan HC AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Ng, Cherrie AU - Ng C AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Lau, Eric H L AU - Lau EHL AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Yeung, Zenon W C AU - Yeung ZWC AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Wong, Eddy W Y AU - Wong EWY AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Leung, Leanne AU - Leung L AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Qu, Xinyu AU - Qu X AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Wang, Daijuanru AU - Wang D AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Cai, Liuyang AU - Cai L AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Chan, Paul K S AU - Chan PKS AUID- ORCID: 0000-0002-6360-4608 AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Chan, Jason Y K AU - Chan JYK AUID- ORCID: 0000-0002-9480-4637 AD - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FAU - Chen, Zigui AU - Chen Z AUID- ORCID: 0000-0002-8577-1298 AD - Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230216 PL - United States TA - Int J Cancer JT - International journal of cancer JID - 0042124 RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Humans MH - Squamous Cell Carcinoma of Head and Neck MH - *Esophageal Squamous Cell Carcinoma MH - *Esophageal Neoplasms/microbiology MH - Dysbiosis/complications MH - RNA, Ribosomal, 16S/genetics MH - Case-Control Studies MH - *Carcinoma, Squamous Cell/pathology MH - *Mouth Neoplasms/microbiology MH - Bacteria/genetics MH - *Head and Neck Neoplasms MH - *Microbiota/genetics MH - Tumor Microenvironment OTO - NOTNLM OT - 16S rRNA OT - esophageal SCC OT - esophageal lavage OT - oral cavity SCC OT - oral rinse OT - upper aerodigestive microbiota EDAT- 2023/02/09 06:00 MHDA- 2023/03/09 06:00 CRDT- 2023/02/08 08:42 PHST- 2023/01/16 00:00 [revised] PHST- 2022/09/22 00:00 [received] PHST- 2023/01/18 00:00 [accepted] PHST- 2023/02/09 06:00 [pubmed] PHST- 2023/03/09 06:00 [medline] PHST- 2023/02/08 08:42 [entrez] AID - 10.1002/ijc.34460 [doi] PST - ppublish SO - Int J Cancer. 2023 May 1;152(9):1903-1915. doi: 10.1002/ijc.34460. Epub 2023 Feb 16.