PMID- 36763216
OWN - NLM
STAT- MEDLINE
DCOM- 20230612
LR  - 20231116
IS  - 1573-2622 (Electronic)
IS  - 0012-4486 (Linking)
VI  - 146
IP  - 3
DP  - 2023 Jun
TI  - Rod photoreceptor activation and deactivation in early-stage diabetic eye 
      disease.
PG  - 229-239
LID - 10.1007/s10633-023-09925-y [doi]
AB  - PURPOSE: To infer rod phototransduction activation and deactivation 
      characteristics in diabetics who have mild or no clinically-apparent retinopathy. 
      METHODS: Fifteen non-diabetic controls, 15 diabetics with no clinically-apparent 
      diabetic retinopathy (NDR), and 15 diabetics with mild non-proliferative diabetic 
      retinopathy (MDR) participated. Dark-adapted flash electroretinograms (3.2 to 4.4 
      log scot td-s) were recorded to assess rod activation. The a-waves were fit with 
      a Gaussian model to derive R(mp3) (maximum photoreceptor response amplitude) and 
      S (phototransduction sensitivity). Rod deactivation was assessed with a paired 
      flash paradigm, in which a-waves were measured for two flashes separated by 
      inter-stimulus intervals (ISIs) of 0.125 to 16 s. The ISI needed for the a-wave 
      amplitude of the second flash to recover to 50% of the first flash (t(50)) was 
      determined. The effect of stimulus retinal illuminance on activation and 
      deactivation was evaluated in a subset of control subjects. RESULTS: Analysis of 
      variance indicated that both diabetic groups had significant log S reductions 
      compared to controls (p < 0.001). Mean S was reduced by approximately 49% and 78% 
      for the NDR and MDR groups, respectively. In contrast, log R(mp3) and log t(50) 
      did not differ significantly among the groups (both p > 0.08). Reducing stimulus 
      retinal illuminance significantly reduced S, but did not significantly affect 
      R(max) or t(50). CONCLUSIONS: Only phototransduction sensitivity was abnormal in 
      this sample of diabetic subjects. The normal deactivation kinetics suggests that 
      circulating rod current is normal. These findings begin to constrain possible 
      explanations for abnormal rod function in early diabetic retinal disease.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - McAnany, J Jason
AU  - McAnany JJ
AUID- ORCID: 0000-0003-1192-3487
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois at 
      Chicago, 1855 W. Taylor St., MC/648, Chicago, IL, 60612, USA. jmcana1@uic.edu.
AD  - Department of Bioengineering, University of Illinois at Chicago, 851 South Morgan 
      St., Chicago, IL, 60607, USA. jmcana1@uic.edu.
FAU - Park, Jason C
AU  - Park JC
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois at 
      Chicago, 1855 W. Taylor St., MC/648, Chicago, IL, 60612, USA.
LA  - eng
GR  - P30 EY001792/EY/NEI NIH HHS/United States
GR  - P30EY001792/National Eye Institute/
GR  - R01EY026004/National Eye Institute/
GR  - R01 EY026004/EY/NEI NIH HHS/United States
GR  - Department/Research to Prevent Blindness/
PT  - Journal Article
DEP - 20230210
PL  - Netherlands
TA  - Doc Ophthalmol
JT  - Documenta ophthalmologica. Advances in ophthalmology
JID - 0370667
SB  - IM
MH  - Humans
MH  - Retinal Rod Photoreceptor Cells/physiology
MH  - Dark Adaptation
MH  - *Diabetic Retinopathy/diagnosis
MH  - Electroretinography
MH  - Photic Stimulation
MH  - *Retinal Diseases
MH  - *Diabetes Mellitus
OTO - NOTNLM
OT  - Diabetic retinopathy
OT  - Electroretinogram
OT  - Photoreceptors
OT  - Rod function
EDAT- 2023/02/11 06:00
MHDA- 2023/06/12 06:42
CRDT- 2023/02/10 11:18
PHST- 2022/09/16 00:00 [received]
PHST- 2023/01/24 00:00 [accepted]
PHST- 2023/06/12 06:42 [medline]
PHST- 2023/02/11 06:00 [pubmed]
PHST- 2023/02/10 11:18 [entrez]
AID - 10.1007/s10633-023-09925-y [pii]
AID - 10.1007/s10633-023-09925-y [doi]
PST - ppublish
SO  - Doc Ophthalmol. 2023 Jun;146(3):229-239. doi: 10.1007/s10633-023-09925-y. Epub 
      2023 Feb 10.